Migratory locust Mucin17 and coding gene and application thereof

A technology of mucin and migratory locust, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as environmental pollution, non-target biological hazards, drug resistance, etc., and achieve the effect of broad application prospects

Active Publication Date: 2019-05-10
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, its control mainly relies on chemical control, but long-term application of chemical insecticides lead

Method used

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  • Migratory locust Mucin17 and coding gene and application thereof
  • Migratory locust Mucin17 and coding gene and application thereof
  • Migratory locust Mucin17 and coding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Acquisition of migratory locust mucin gene sequence

[0014] 1. Acquisition of the mucin gene sequence of migratory locusts

[0015] Based on the whole locust transcriptome database (GenBank accession number: GEZB00000000) obtained by our laboratory, the mucin gene (Mucin) of migratory locust was searched by bioinformatics method, and the partial sequence of mucin gene Mucin17 of migratory locust was obtained. The NCBI website was used to perform Blast analysis on the sequence, and at the same time combined with the genome sequence of migratory locust (GenBank accession number: AVCP000000000) to obtain its coding sequence, and after splicing and comparison using BioEdit software, the full-length open reading frame sequence of mucin gene Mucin17 of migratory locust was obtained . According to the obtained full-length open reading frame sequence of migratory locust mucin gene Mucin17, primer premier 5.0 software was used to design 4 pairs of upstream and do...

Embodiment 2

[0022] Embodiment 2: Migratory locust lethal experiment of mucin gene dsRNA

[0023] 1. Injection of migratory locust mucin gene dsRNA

[0024] Thirty 5th-instar and 2-day-old nymphs with healthy growth and uniform size were selected for the experiment. 5 μl (10 μg) of dsRNA of SEQ ID NO: 3 was gently injected with a 25 μl micro-syringe along the blood flow between the second and third abdominal segments of the lateral abdomen of the nymph. At the same time, 30 nymphs were selected as the control group. Inject the same volume and concentration of dsGFP into the control group. The injected migratory locusts were reared in a 30°C constant temperature biochemical incubator (light:dark time=14h:10h, temperature 30±2°C, humidity 60%), and fed fresh wheat seedlings and wheat bran every day.

[0025] 2. Detection of mucin gene silencing in migratory locusts

[0026]Nine nymphs were collected 24 hours after injection of dsGFP and dsRNA, and the wing bud tissue was dissected for to...

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Abstract

The invention discloses a migratory locust Mucin17 and a coding gene and application thereof. A migratory locust mucin gene Mucin17 is cloned from a migratory locust, and a primer is designed for themigratory locust mucin gene Mucin17, and dsRNA used for interfering the migratory locust mucin gene Mucin17 is synthesized, and the dsRNA is guided into the migratory locust through an injection method to conduct RNAi on the migratory locust mucin gene Mucin17. The result shows that after effective silencing is conducted on the migratory locust mucin gene Mucin17, death of the migratory locusts can be caused, and development of wings and hind feet of the migratory locusts is affected. Accordingly, a new target is provided for further deeply understanding the action mechanism of the mucin development of the migratory locusts, and for pest control based on RNA interference, and the migratory locust Mucin17 and the coding gene and application thereof have the theoretical significance and wideapplication prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to mucin 17 of migratory locust, its coding gene and application thereof. Background technique [0002] Migratory locust is an intercontinental agricultural pest, mainly distributed in Asia, Europe, Africa, and Australia, and distributed in most parts of my country. It mainly threatens corn, millet and other gramineous crops. In the case of starvation, it also eats soybeans, cabbage and sunflowers. The migratory locust has jumping and powerful front and rear wings, and has the physiological function of long-distance and large-scale migration, making its activity space expand from plane to three-dimensional, and its action radius is greatly increased. Efficiency and other aspects have been greatly improved. Therefore, once the locust plague breaks out, it will have a very serious impact on my country's food security and agricultural income growth. At present, its control mainly relies...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12C12N15/113A01N57/16A01P7/04
Inventor 杨青赵小明
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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