Unlock instant, AI-driven research and patent intelligence for your innovation.
Kit for rapidly detecting methicillin-resistant staphylococcus aureus and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for methicillin-resistant Staphylococcus aureus, applied in the field of rapid detection kits for methicillin-resistant Staphylococcus aureus, to achieve rapid detection, low cost, and high throughput
Pending Publication Date: 2016-03-09
HANGZHOU DIAN BIOTECH CO LTD
View PDF0 Cites 4 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, not all MRSAs carry the mecA gene. Therefore, when identifying MRSA based on the mecA gene, there are often false negative results.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0032] Example 1: Detection of the nucleic acid sequence of methicillin-resistant Staphylococcus aureus gene detection.
[0033] 2 pairs of specific primers and 2 probes were entrusted to Yingwei Jieji (Shanghai) Trading Co., Ltd. to synthesize:
[0034] F1 (SEQ ID NO: 1): 5'-tataacaacatgaaaaatgattatgg-3';
[0035] R1 (SEQ ID NO: 2): 5'-catatgaaggtgtgcttacaagtgc-3';
[0036] F2 (SEQ ID NO: 3): 5'-gctgatggaaaaatggtaaacga-3';
[0037] R2 (SEQ ID NO: 4): 5'-ctcttttttcgcttgtgcttca-3';
[0038] T1 (SEQ ID NO: 5): Fam-5'-aggtactgctatccaccctcaaacaggtga-3'-tamra;
[0039] T2 (SEQ ID NO: 6): hex-5'-gttcgtcaaggcttggctaaagttgctt-3'-tamra.
[0044] (1) Preparation of bacterial genomic DNA template: refer to published literature, different types of bacterial specimens, prepare bacterial genomic DNA according to the kit instructions, and use it as a PCR reaction template for future use.
[0045] (2) using the genomic DNA described in step (1) as a template, using 2 pairs of specific primers and 2 probes to carry out the amplification detection of the drug-resistant gene mecA of methicillin-resistant Staphylococcus aureus, specifically comprising the following steps;
[0046] (a) Preparation of PCR reaction solution: Take out the components of the kit from the -20°C refrigerator, thaw at room temperature, and put them on an ice box for la...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a primer for detecting methicillin-resistant staphylococcus aureus. The primer comprises a primer body for amplifying a methicillin-resistant mecA medicine resistant gene, a primer body for amplifying a staphylococcus aureus nuc gene, a fluorescent probe T1 for detecting the methicillin-resistant mecA medicine resistant gene and a fluorescent probe T2 for detecting the staphylococcus aureus nuc gene. The nucleotide sequence is shown as SEQ ID NO:1-6. The invention further discloses a kit comprising the primer for detecting the methicillin-resistant staphylococcus aureus. The primer and the kit are suitable for rapidly detecting methicillin-resistant staphylococcus aureus strains in a suspicious staphylococcus aureus infected person clinic sample, and have the advantages of being high in sensitivity and specificity, stable, timely, convenient to operate and the like.
Description
technical field [0001] The invention belongs to the technical field of microbial detection, and in particular relates to a rapid detection kit for methicillin-resistant Staphylococcus aureus and an application thereof. Background technique [0002] Staphylococcus aureus (SAU) is one of the most common pathogens causing nosocomial infections. With the widespread use of a large number of broad-spectrum antibacterial drugs in clinical practice, the drug resistance of Staphylococcus aureus in hospital infections is also increasing. In recent years, nosocomial infections The isolation rate of methicillin-resistant Staphylococcus aureus (MRSA) has been at a high level. [0003] The mecA gene is a foreign gene obtained by Staphylococcus aureus through the transposition mechanism, encoding and synthesizing PBP2α. The mecA gene not only mediates the drug resistance of Staphylococcus aureus to β-lactamantibiotics, but also the DNA fragment SCCmec where the mecA gene is located can c...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
