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Separation preparation and application of compound with antimicrobial activity to fungus secondary metabolic product

A compound and fermentation product technology, applied in the field of medicine, can solve problems such as increased medical expenses, increased mortality, and patient treatment failures

Active Publication Date: 2019-04-09
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacterial drug resistance directly leads to patient treatment failure, increased medical expenses, and increased mortality. What is more serious is that the further development of drug-resistant bacteria may make human beings face the threat of infectious diseases again

Method used

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  • Separation preparation and application of compound with antimicrobial activity to fungus secondary metabolic product
  • Separation preparation and application of compound with antimicrobial activity to fungus secondary metabolic product
  • Separation preparation and application of compound with antimicrobial activity to fungus secondary metabolic product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation and structure identification of compounds 1-5.

[0024] (1) Preparation of compound 1 as shown in formula (1)

[0025] 1. Fermentation conditions

[0026] Preparation of seed culture solution: Take 3.0g of yeast extract, 3.0g of malt extract, 5.0g of peptone, and 10.0g of glucose, dissolve them in an appropriate amount of water, then dilute to 1000mL with water, sterilize at 121°C for 30 minutes, and set aside. Aspergillus TJ23 is inoculated into the above-mentioned medium, and cultured on a shaker under the conditions of 25-28° C. and 100-120 rpm for 3-5 days to obtain a seed culture solution.

[0027] Fermentation: put 200g of rice into a 1000mL Erlenmeyer flask, add 200mL of water, sterilize at 121°C for 30min, and set aside. The above seed culture solution is inoculated into the rice culture medium, and cultured statically for 25-30 days under the condition of 25-28°C.

[0028] 2. Extraction and separation

[0029] The fermented product ob...

Embodiment 2

[0043] Example 2: See Table (3) for the antibacterial activity of compounds 1-5.

[0044] Table (3). Antibacterial activity of compounds 1-5 (MIC, μg / mL)

[0045]

[0046] a MRSA = methicillin-resistant Staphylococcus aureus ATCC 43300; b S. aureus = Staphylococcus aureus ATCC25923; c E. faecalis = Enterococcus faecalis ATCC 29212; d ESBL-E.coli=Escherichia coli ATCC 35218 producing extended-spectrum β-lactamase; e E.coli = Escherichia coli ATCC25922; f P. aeruginos = Pseudomonas aeruginosa ATCC 15442; g K. pneumonia = Klebsiella pneumoniae ATCC 700603; h The selection of recruiting product refers to CLSI standard (National Committee for Clinical Laboratory Standards, CLSI); Va=vancomycin; Ch=chloramphenicol; Am=amikacin; Ce=ceftriaxone.

[0047] Drug-resistant strains and control substances: The drug-resistant strains tested for antibacterial activity were purchased from the ATCC standard strain library, and the selection of control substances was in accordance with...

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PUM

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Abstract

The invention belongs to the technical field of medicines and discloses (1) activity and structures of new meroterpenoid type compounds 1 to 5 derived from a fungus secondary metabolic product, (2) aseparation and purification preparation method and antimicrobial activity of the compounds 1 to 5 and structural formulas of the compounds 1 to 5, and (3) derivative analogues. The compound 1 and thecompound 3 have relatively strong antimicrobial activity on methicillin-resisting staphylococcus aureus, staphylococcus, escherichia coli capable of producing extended-spectrum beta-lactamase, escherichia coli and pseudomonas aeruginosa.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to the separation and preparation method and application of compound 1-5. It specifically involves the separation and purification process, structure confirmation, antibacterial effect, etc. Background technique [0002] Bacterial drug resistance is a hot issue of global concern in this century and has become a serious public health crisis. Bacterial drug resistance directly leads to patient treatment failure, increased medical expenses, and increased mortality. What is more serious is that the further development of drug-resistant bacteria may make human beings face the threat of infectious diseases again. At present, strengthening the research and development of antibacterial drugs is the consensus at home and abroad to combat bacterial resistance. [0003] The richness of fungal metabolites and the diversity of their biological activities are an inexhaustible driving force for t...

Claims

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Application Information

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IPC IPC(8): C07D307/94C07D493/10C07J71/00C07J73/00C12P17/18C12P17/04C12P33/20A61P31/04C12R1/66
CPCC07D307/94C07D493/10C07J71/0005C07J73/003C12P17/04C12P17/181C12P33/20Y02A50/30
Inventor 张勇慧何艳乔玉本李琴朱虎成胡正喜薛永波汪建平
Owner HUAZHONG UNIV OF SCI & TECH
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