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Bacillus strain capable of efficiently degrading DMP (dimethyl phthalate), culture method and application thereof to remediation of soil PAEs (phthalic acid esters) pollution

A technology of Bacillus sp. and Bacillus subtilis, applied in the field of microorganisms, can solve problems such as affecting the function of black soil ecosystem, threatening black soil soil fertility and food production, changing black soil respiration and enzymatic activity and metabolic characteristics, etc.

Active Publication Date: 2015-09-23
QIQIHAR UNIVERSITY +1
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Problems solved by technology

Some research results show that DMP pollution can change the metabolic characteristics of black soil respiration and enzyme activity, affect the ecosystem function of black soil, and then threaten the soil fertility and food production of black soil

Method used

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  • Bacillus strain capable of efficiently degrading DMP (dimethyl phthalate), culture method and application thereof to remediation of soil PAEs (phthalic acid esters) pollution
  • Bacillus strain capable of efficiently degrading DMP (dimethyl phthalate), culture method and application thereof to remediation of soil PAEs (phthalic acid esters) pollution
  • Bacillus strain capable of efficiently degrading DMP (dimethyl phthalate), culture method and application thereof to remediation of soil PAEs (phthalic acid esters) pollution

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Experimental program
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Embodiment 1

[0023] Example 1 Isolation of bacterial strain QD-9-10 and its application in degrading PAEs

[0024] 1 Materials and methods

[0025] 1.1 Test materials and medium

[0026] Dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), di-n-octyl phthalate (DOP), di-phthalate Isooctyl ester (DEHP), and diisooctyl phthalate (DIOP), methanol, ethyl acetate, and hexane, all chemicals and solvents were analytical reagents, purchased from Guangfu Institute of Fine Chemicals, Tianjin.

[0027] Basic mineral salt (MSM) medium: K 2 HPO 4 12H 2 O 1.0g, KH 2 PO 4 1.0g, NH 4 Cl 0.8g, NaCl 1.0g, MgSO 4 ·7H 2 O 0.5g, CaCl 2 0.006g, FeSO 4 ·7H 2 O 0.02g, trace element stock solution (MnSO 4 ·H 2 O 0.1g, ZnSO 4 ·7H 2 O 0.12g, H 3 BO 3 0.07g, Na 2 MoO 4 ·H 2 O 0.04g, CuSO 4 ·5H 2 O 0.02g, CoCl 2 0.04g, distilled water to make up to 1000ml) 2mL, distilled water to make up to 1000ml, pH 7.0.

[0028] LB medium: peptone 10.0g, yeast powder 5.0g, sodium ...

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Abstract

The invention discloses a bacillus strain capable of efficiently degrading DMP (dimethyl phthalate), a culture method and application thereof to remediation of soil PAEs (phthalic acid esters) pollution. A strain QD-9-10 which grows by mainly taking DMP as a carbon source is separated and identified from black soil covered with mulching films for a long term by adopting inorganic salt culture medium. According to morphological characteristic observation of a colony and 16SrDNA base sequence determination and homological analysis, the identification result of the bacterium is that QD-9-10 belongs to Bacillus subtilis. According to results of orthogonal experiments, the optimum culture method of the strain QD-9-10 is to perform culture by adopt inorganic salt culture medium under culture conditions that pH is 8.0, temperature is 35DEG C and shaking speed is 150rpm and DMP initial concentration is 100mg L<-1>, and biomass reaches the maximum at 32h. In addition, according to substrate utilization tests, the strain QD-9-10 can also utilize other common PAEs. Accordingly, the Bacillus subtilis QD-9-10 strain has a capability of degrading DMP and other common PAEs, and has a certain application prospect in aspects of degrading PAEs pollutants and remedying soil PAEs pollution.

Description

technical field [0001] The invention relates to a PAEs degrading bacterium and its use, in particular to a Bacillus strain capable of efficiently degrading DMP and its use in degrading PAEs and remediating soil PAEs pollution. The invention belongs to the technical field of microorganisms. Background technique [0002] Phthalates (PAEs) are organic compounds widely used in plastic processing and many other industrial consumer products. The widespread use of phthalates has resulted in more and more phthalates entering our ecosystem. The environmental and human health problems caused by PAEs pollution have aroused widespread concern. Due to the carcinogenic, teratogenic and mutagenic effects of PAEs (Song Jiaoyan, Liu Dajun, Wan Yang, et al. Study on kinetics and infrared spectrum characteristics of humic acid adsorption of dibutyl phthalate[J]. Southwest University Journal (Nature Science Edition), 2014,3:022.), the U.S. Environmental Protection Agency has listed 6 PAEs as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/125A62D101/28
CPCA62D3/02A62D2101/28B09C1/10C12N1/20C12N1/205C12R2001/125
Inventor 王志刚徐伟慧莫继先胡云龙胡影刘帅王春龙
Owner QIQIHAR UNIVERSITY
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