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Tobacco seed-stage morphological classification characterization method based on sample consensus

A morphological classification and consistency technology, applied in the field of classification and characterization of biological characteristics of plant growth period, can solve problems such as difficult to meet experimental requirements, difficult to achieve accurate, effective and unified effects, and achieve easy operation, accurate reflection, Effectiveness of good biological assays

Inactive Publication Date: 2013-04-10
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological experiments require that the collected samples have a high degree of consistency. The traditional division of flue-cured tobacco species is difficult to achieve accurate, effective, and uniform results, and it is difficult to meet the experimental requirements.

Method used

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  • Tobacco seed-stage morphological classification characterization method based on sample consensus
  • Tobacco seed-stage morphological classification characterization method based on sample consensus
  • Tobacco seed-stage morphological classification characterization method based on sample consensus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The common flue-cured tobacco Safflower Dajinyuan seed period was selected, and based on the sampling consistency, the classification and characterization of the species stage morphology was carried out.

[0022] Taking the collection of biological samples at the budding stage of Safflower Dajinyuan as an example, according to the present invention, the characterization method for the classification of the common flue-cured tobacco Safflower Dajinyuan species stage morphology comprises the following steps:

[0023] Tobacco plants listed. Select 100 tobacco plants with the same growth in the later period of field flue-cured tobacco, and number and list them sequentially. Morphological classification and characterization of flue-cured tobacco at the budding stage. The tobacco plants required for sample collection were selected from the listed tobacco plants.

[0024] Combined with the traditional method of dividing the seeding period, according to the growth of flue...

Embodiment 2

[0029] Taking the collection of biological samples at the blooming stage of safflower and Dajinyuan as an example, according to the present invention, the characterization method for the species classification of common flue-cured tobacco safflower and Dajinyuan is adopted, comprising the following steps:

[0030] Tobacco plants listed. With embodiment 1.

[0031] Combining with the traditional division method of seeding period, according to the growth of flue-cured tobacco in the field period of about 60 days, the central flower of about 50% of the tobacco plants is visually opened, indicating that the tobacco plants have entered the full flowering period, and sampling can be started at the full flowering period.

[0032] Randomly select 10 plants from the remaining 90 listed tobacco plants, count the proportion of flower buds, flowers and fruits of each plant, and select the proportion that meets the requirements of flower buds:flowers:fruits: (50~10):(50~90):(0 ~1...

Embodiment 3

[0035] Taking the collection of biological samples at the maturity stage of China Tobacco 100 capsules as an example, according to the present invention, the characterization method for the morphological classification of 100 stages of common flue-cured tobacco in China Tobacco comprises the following steps:

[0036] Tobacco plants listed. With embodiment 1.

[0037] Combining with the traditional division method of seeding period, according to the growth of flue-cured tobacco in the field period of about 80 days, about 70% of the tobacco plants have matured capsules visually, indicating that the tobacco plants have entered the capsule maturity period, and sampling can be started at the capsule maturity period.

[0038] Randomly select 10 tobacco plants from the listed tobacco plants, count the ratio of green fruit and mature capsule for each plant, and select period VII sample tobacco plants that meet the ratio of green fruit: mature capsule (30-0): (70-100) for samp...

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Abstract

The invention discloses a tobacco seed-stage morphological classification characterization method based on sample consensus. The method is characterized by comprising the following steps: (1), selecting 100 tobacco plants, consecutively numbering and hanging plates, selecting morphological classification characterization samples at different seed stages from the plate-hung tobacco plants; (2), combining with the conventional classification of the tobacco seed stage, roughly characterizing; (3), quantitative classification characterization: performing the quantitative classification characterization according to different ratios of buds, flowers and fruits of the tobacco plants, classifying the tobacco seed-stage into 6 phases; and (4), sampling the tobacco plant samples at different phases from the tobacco plants within the quantitative classification range suitable for the phases, so as to meet the requirements of sample consensus. Compared with the existing classification method, the method adopts different ratios of visual buds, flowers and fruits, quantizes the objective morphological characterization of the tobacco seed stage, can accurately classify in standard, be easily operated, and beneficial to collect uniform tobacco seed-stage samples, so as to better analyze and determine the biological information of the samples, and more accurately reflect the actual information of the samples.

Description

[0001] technical field [0002] The invention relates to the field of classification and characterization of biological characteristics of plant growth stages, in particular to a method for classification and characterization of flue-cured tobacco species stage morphology based on sampling consistency. Background technique [0003] Flue-cured tobacco is generally called the seeding period from budding to capsule maturity, about 25 to 30 days. The traditional classification method divides the seeding stage into six stages: budding, budding, flower opening, flower blooming, flower apoptosis, and capsule maturity. Have a better guiding role. However, the traditional classification of each stage of flue-cured tobacco seeding is only carried out by visual inspection, which is very extensive. In fact, the flue-cured tobacco seed stage changes very quickly, for example, it only takes about 2 days from the flowering stage to the flowering stage; moreover, the different stages of t...

Claims

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Application Information

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IPC IPC(8): G01N21/00
Inventor 李锋杨军魏春阳王燃罗朝鹏林福呈金立锋曹培健
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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