This invention relates to
plant genetic engineering, and more particularly to a variegated
Phalaenopsis orchid and its preparation method, the preparation method comprising the following steps: (1) according to the
target gene with the
base sequence shown in SEQ ID No. 1 PePDS (1) Synthesize specific
nucleotide fragments; (2) Construct specific
nucleotide fragments into a
plant virus vector; (3) Introduce the recombinant
plant virus vector obtained in step (2) into
Phalaenopsis orchid plant material; (4) Screen to obtain
Phalaenopsis orchids with silenced target genes. This invention achieves rapid, efficient, and non-transgenic leaf color
variegation creation in commercial Phalaenopsis orchid varieties for the first time through VIGS technology; it not only breaks through the technical
bottleneck of difficult and long cycle of genetic manipulation of orchid plants, significantly shortening the trait
verification cycle, but also induces diverse and highly ornamental variegated leaf phenotypes such as albino, yellow, or purple in different varieties, and has the advantages of simple operation, low cost, and industrialization feasibility.