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A strain of Aspergillus versicolor hy12

A technology of Aspergillus versicolor and strains, applied in the field of Aspergillus versicolor HY12 strain, to achieve the effects of high lethality and teratogenicity, rapid growth and reproduction, and strong infectivity

Active Publication Date: 2020-08-14
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, it is essential to study the growth and reproduction characteristics of Spodoptera litura, to provide a strain with large reproduction number, fast speed, and capable of infecting Spodoptera litura, to inhibit the growth and reproduction of Spodoptera litura, and to provide an experimental basis for preventing and controlling Spodoptera litura Problems urgently needed to be solved by field technicians

Method used

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  • A strain of Aspergillus versicolor hy12
  • A strain of Aspergillus versicolor hy12
  • A strain of Aspergillus versicolor hy12

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Screening of Aspergillus versicolor HY12 strain

[0022] 47 soil samples were collected from the Spodoptera litura infested areas in southern Hunan tobacco fields, including 25 soil samples in Jianghua, Yongzhou, 16 soil samples in Hengyang, 4 soil samples in Jiangyong, and 2 soil samples in Ningyuan. Among them, the 5-point sampling method is used to collect soil samples, and the center distance between the two tobacco plants is selected as the soil sampling point, and the soil samples with a length of 10 cm, a width of 10 cm, and a depth of 5 cm are cut down with a soil shovel and put into a sterile self-sealing plastic bag. Take it back to the laboratory and store it in the refrigerator for separation. The time, location and related information of sample collection shall be recorded in detail, and different soil samples shall be marked separately.

[0023] The retrieved tobacco field soil samples were crushed under aseptic conditions and fully mixed, then fully mixe...

Embodiment 2

[0042] Identification of Aspergillus versicolor HY12 strain

[0043] Inoculate the Aspergillus strain (HY12) obtained in Example 1 onto the SDAY medium, cultivate it in a constant temperature incubator at 25.6°C, observe its colony character and color, and pick mycelium from the colony to make a microscopic examination, observe The color and properties of the mycelia, conidiophores and sporulation axes; after the colony produced sporulation, the conidia were picked up for microscopic examination to observe the conidia’s properties, color, size, and photograph. The results were as follows: figure 1 shown. Depend on figure 1 It can be seen that after culturing on SDAY medium for 14 days, the diameter of the colony reaches 66.3mm, the colony is round, white cotton-like mycelia, the colony is thicker, and the texture is dense and soft; a large number of conidia are produced in the later stage, and the spores are brown powdery, and the powder layer Thick, with concentric ridges. ...

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Abstract

The invention provides a strain of Aspergillus versicolor HY12, the colony of Aspergillus versicolor HY12 is round, dense and soft in texture, mycelium is in the shape of white cotton wool, and can produce a large number of conidia. The wheel pattern is raised. The invention provides a strain of Aspergillus versicolor HY12, which has extremely high growth and reproduction speed in a short period of time, can produce a large number of conidia, can infect Spodoptera litura, and has a relatively high lethality and teratogenicity to Spodoptera litura The rate will lay the experimental foundation for the control of Spodoptera litura in the later stage, and provide a favorable reference for the control of other pests in this field.

Description

technical field [0001] The invention relates to the field of biology, in particular to a strain of Aspergillus versicolor HY12. Background technique [0002] Since the invention of chemical pesticides, chemical pesticides have grown at an astonishing rate of 5% per year. Although in the past few decades, chemical pesticides have played an important role in the increase of agricultural crop yield and pest control, the extensive use of chemical pesticides has brought about serious "3R" problems (i.e. drug resistance, pesticide residue Residence, No matter how rampant Resurgence is, it has also brought a series of ecological and environmental problems. The long-term and extensive use of chemical pesticides has led to an increase in pesticide-resistant pests, especially in the past 10 years, the resistance of cotton bollworms, aphids, diamondback moths, Spodoptera litura, and spider mites to pyrethroids and organophosphorus chemical pesticides has increased hundreds or even th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/66
CPCC12N1/145C12R2001/66
Inventor 谭琳汤心砚胡秋龙刘双清柏连阳程予奇
Owner HUNAN AGRICULTURAL UNIV
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