Modified human acidic fibroblast growth factor gene and recombinant vector and applications thereof

A fibroblast and growth factor technology, applied in the field of genetic engineering, can solve problems affecting application, environmental pollution, and increased production costs, and achieve the effect of promoting cell proliferation and reducing production costs

Active Publication Date: 2015-04-15
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of these new silks requires the use of acid, alkali, high temperature and other conditions to remove the sericin co

Method used

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  • Modified human acidic fibroblast growth factor gene and recombinant vector and applications thereof
  • Modified human acidic fibroblast growth factor gene and recombinant vector and applications thereof
  • Modified human acidic fibroblast growth factor gene and recombinant vector and applications thereof

Examples

Experimental program
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Embodiment Construction

[0022] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0023] The cell line used in the examples is mouse fibroblast NIH / 3T3, which is preserved by the State Key Laboratory of Bombyx mori Genome Biology, Southwest University, China.

[0024] 1. Preparation of human acidic fibroblast growth factor (hFGF1) gene transgenic silkworm

[0025] A codon-optimized human acidic fibroblast growth factor (hFGF1) gene will be commercially synthesized, that is, a modified human acidic fibroblast growth factor gene, the nucleotide sequence of which is shown in SEQ ID NO.1. The upstream of the nucleotide shown in SEQ ID NO.1 is the BamHI res...

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PUM

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Abstract

A modified human acidic fibroblast growth factor gene is disclosed. The nucleotide sequence of the gene is shown as the number 25 locus to the number 450 locus of SEQ ID NO.1. The sequence is designed according to codon preferences of bombyx mori, and can be normally expressed and translated in the bombyx mori. A recombinant vector containing the modified human acidic fibroblast growth factor gene is also disclosed. The recombinant vector comprises an enhanser hr3 and a secreting type sericin 1 promoter Ser1, specifically expresses a modified human acidic fibroblast growth factor in a middle silk gland cell of the bombyx mori, and secretes a recombinant protein to a sericin layer of silk. The obtained silk has bioactivity, has a function of promoting cell proliferation when the silk is directly used, and is a good medical material.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and particularly relates to a modified human acidic fibroblast growth factor gene, and also relates to a recombinant vector containing the modified human acidic fibroblast growth factor gene and an application of the modified human acidic fibroblast growth factor gene. Background technique [0002] Bombyx mori is famous for producing silk, and is one of the earliest economic animals (insects) fully domesticated and utilized by humans. The silk gland of the silkworm is the organ that synthesizes and secretes silk protein, and is the biological basis of the whole silk industry. After thousands of years of artificial domestication, the silk glands of the silkworm have a super strong ability to synthesize and secrete proteins. A silkworm weighing about 5g can synthesize and secrete about 0.5g of silk protein, which is the most among known insects. Silk protein is mainly composed of fibroin and ser...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/85A01K67/04A61L27/22
Inventor 夏庆友王峰徐汉福王元成袁林马三垣赵萍
Owner SOUTHWEST UNIVERSITY
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