Jack fruit pulp freezing and slicing method

A frozen section and jackfruit technology, which is applied in the preparation of test samples, sampling devices, etc., can solve the problems of inability to observe the morphology of pulp tissue, incomplete cell fragmentation structure, etc., to save experimental time, reduce experimental steps, and reduce fragmentation. Effect

Pending Publication Date: 2019-11-12
TROPICAL FRUIT RES INST HAINAN ACADOF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Jackfruit pulp contains a lot of water, and ice crystals are prone to appear during general frozen sections, resulting in incomplete cell fragmentation and structure, and even the shape of the entire pulp tissue cannot be observed

Method used

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  • Jack fruit pulp freezing and slicing method

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A method for frozen sectioning of jackfruit pulp, comprising the following steps:

[0042] (1) Get the fresh jackfruit pulp and cut it into small pieces, and place the small pieces of pulp tissue in the FAA fixative solution containing 18% sucrose and process it for 25min, then fix the pulp tissue with a concentration of 18% The phosphate buffer solution of sucrose was soaked for 20min, and the concentration of the phosphate buffer solution was 8mmol / L.

[0043] (2) Place the soaked pulp tissue in 10% glycerol, and vacuum it for 15 minutes.

[0044] (3) Place the vacuum-pumped pulp tissue in an incubator for 2 hours at a temperature of 20°C, and then embed it with a mixed embedding agent of sucrose solution and OTC at a volume ratio of 0.8:1.2 for 20 minutes.

[0045] (4) Place the embedded pulp tissue in liquid nitrogen for quick freezing for 35 seconds, and the quick freezing temperature is -190°C.

[0046] (5) Place the quick-frozen pulp tissue in a freezer for 0.8...

Embodiment 2

[0049] A method for frozen sectioning of jackfruit pulp, comprising the following steps:

[0050](1) Get the fresh jackfruit pulp and cut it into small pieces, and place the small pieces of pulp tissue in the FAA fixative solution containing 23% sucrose to process for 35min, then fix the pulp tissue with a concentration of 18~ The 23% sucrose phosphate buffer was soaked for 30 minutes, and the concentration of the phosphate buffer was 12 mmol / L.

[0051] (2) Place the soaked pulp tissue in 20% glycerin, and vacuum it for 25 minutes.

[0052] (3) Place the vacuum-pumped pulp tissue in an incubator for 4 hours at a temperature of 25°C, and then embed it with a mixed embedding agent of sucrose solution and OTC at a volume ratio of 1.2:0.8 for 40 minutes.

[0053] (4) Place the embedded pulp tissue in liquid nitrogen for quick freezing for 45 seconds, and the quick freezing temperature is -210°C.

[0054] (5) Place the quick-frozen pulp tissue in a freezer for 1.2 hours and then...

Embodiment 3

[0057] A method for frozen sectioning of jackfruit pulp, comprising the following steps:

[0058] (1) Get the fresh jackfruit pulp and cut it into small pieces, and place the small pieces of pulp tissue in the FAA fixative solution containing 20% ​​sucrose for 30 minutes, then fix the processed pulp tissue with a concentration of 20%. The phosphate buffer solution of sucrose was soaked for 15min, and the concentration of the phosphate buffer solution was 10mmol / L.

[0059] (2) Place the soaked pulp tissue in 15% glycerin, and vacuum for 50 minutes.

[0060] (3) Place the vacuum-pumped pulp tissue in an incubator for 3 hours at a temperature of 22°C, and then embed it with a mixed embedding agent of sucrose solution and OTC at a volume ratio of 1:1 for 30 minutes.

[0061] (4) Place the embedded pulp tissue in liquid nitrogen for quick freezing for 40 seconds, and the quick freezing temperature is -200°C.

[0062] (5) Place the quick-frozen pulp tissue in a freezer to warm up...

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Abstract

The invention discloses a jack fruit pulp freezing and slicing method. The jack fruit pulp freezing and slicing method comprises the following steps of carrying out fixing treatment on jack fruit pulpby using a sucrose-FAA fixing solution, and then performing immersion cleaning by using a phosphoric acid buffer solution of sucrose for carrying out infiltration protection on the fixed sample tissues; and putting into glycerol, carrying out vacuum extraction, culturing by a culture box, jointly treating the jack fruit pulp sample by using a mixed embedding agent, putting the embedded sample into liquid nitrogen for quick-freezing, putting the quick-frozen sample in a freezing chamber for temperature rise, and carrying out slicing and dyeing. According to the method disclosed by the invention, all steps are closely related and mutually cooperated, the operation steps of the existing freezing slicing method are optimized, the experimental steps are reduced, the experiment time is shortened, the generation of ice crystal of the jackfruit pulp cells is reduced, the breaking of cells is reduced, the integrity of the cell structure and the tissue structure is guaranteed, and a clear pulptissue slicing diagram can be obtained.

Description

technical field [0001] The invention relates to the technical field of frozen sections, in particular to a method for frozen sectioning of jackfruit pulp. Background technique [0002] Jackfruit: also known as jackfruit, also known as bracts, wood pineapple, tree pineapple, big tree pineapple, honeydew melon, and tripe fruit. The fruit is plump and soft, sweet and delicious, and has a strong fragrance, so it is known as the "Queen of Tropical Fruits". It is a world-famous tropical fruit, belonging to the genus Moraceae and osmanthus genus evergreen trees. It mostly grows near the equator. It has a faint odor. The mature pulp is yellow, sweet and delicious. Contains sugar, fat, protein, deep sweet taste, plump and soft, sweet and delicious, rich fragrance, the fruit of jackfruit fruit is light yellow, has the effects of quenching thirst, clearing milk, nourishing the middle and nourishing qi, and the nutritional value of jackfruit is very high. At the same time, jackfruit al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/06G01N1/42G01N1/30
CPCG01N1/06G01N1/30G01N1/42
Inventor 赵亚范鸿雁冯学杰胡福初王祥和郭利军罗志文陈哲
Owner TROPICAL FRUIT RES INST HAINAN ACADOF AGRI SCI
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