Rapid propagation method for suspension cell culture for savatier monochasma herb

A technology of suspension cells and velvet grass, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of low pollution, high proliferation rate, and large-scale planting

Inactive Publication Date: 2014-12-24
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, velvet grass has not been artificially propagated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take velvet grass leaves, wipe them clean, soak them in washing powder for 4 minutes, wash them with running water for 25 minutes, treat them with 0.1% mercuric chloride on an ultra-clean workbench for 6 minutes, rinse them with sterile water 5 times, and insert the sterilized leaves into VR+NAA0. 01mg / L+ZT3mg / L+1mg / L nystatin medium for callus induction, add 5.5g / L agar, 30g / L sucrose, temperature 26°C, the induced callus is inserted into medium VR Suspension cell culture in +ZT0.3mg / L+KT0.2mg / L+L-glutamine 6mmol / L+sodium oxalate 50mg / L, add 30g / L sucrose, light 5500lx, light time 14h / d, temperature 26℃ , the growth rate of suspension cells was 56%.

Embodiment 2

[0011] Take velvet grass leaves, wipe them clean, soak them in washing powder for 4 minutes, wash them with running water for 25 minutes, treat them with 0.1% mercuric chloride on an ultra-clean workbench for 6 minutes, rinse them with sterile water 5 times, and insert the sterilized leaves into VR+NAA0. 01mg / L+ZT3mg / L+1mg / L nystatin medium for callus induction, add 5.5g / L agar, 30g / L sucrose, temperature 26°C, the induced callus is inserted into medium VR Suspension cell culture in +ZT0.5mg / L+KT0.3mg / L+L-glutamine 8mmol / L+sodium oxalate 100mg / L, add 30g / L sucrose, light 5500lx, light time 14h / d, temperature 26℃ , the growth rate of suspension cells was 57%.

Embodiment 3

[0013] Take velvet grass leaves, wipe them clean, soak them in washing powder for 4 minutes, wash them with running water for 25 minutes, treat them with 0.1% mercuric chloride on an ultra-clean workbench for 6 minutes, rinse them with sterile water 5 times, and insert the sterilized leaves into VR+NAA0. 01mg / L+ZT3mg / L+1mg / L nystatin medium for callus induction, add 5.5g / L agar, 30g / L sucrose, temperature 26°C, the induced callus is inserted into medium VR Suspension cell culture in +ZT0.4mg / L+KT0.3mg / L+L-glutamine 7mmol / L+sodium oxalate 100mg / L, add 30g / L sucrose, light 5500lx, light time 14h / d, temperature 26℃ , the growth rate of suspension cells was 58%.

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Abstract

The invention discloses a rapid propagation method for suspension cell culture for savatier monochasma herb, and the rapid propagation method comprises the steps as follows: obtaining the sterile blades, inducing the callus tissue and cultivating suspension cells; the savatier monochasma herb suspension culture growth rate is high and the growth is stable, and the rapid propagation method for suspension cell culture for savatier monochasma herb is the much more direct route for developing and using medicinal resources.

Description

technical field [0001] The invention relates to a rapid propagation method for suspension cell culture of velvet antler, and belongs to the field of biotechnology. Background technique [0002] velvet grass, Monochasma sheareri Maxim.ex Franch. et Savat. , Scrophulariaceae, aliases: Millennium Ai, Qianzhongta, Pinger Centipede Grass, etc., perennial herb, with lignified taproot, numerous stems, dense clusters, more or less green overall, a small amount of wool on the lower part, very short internodes, pubescent or glabrous, alternately opposite leaves, sometimes alternate, scale-like leaves at the lower part of the stem, overlapping each other in an imbricate shape due to short internodes, gradually growing upward, strip-shaped or strip-lanceolate, single flower Born in the leaf axils on the upper part of the stem, it is in a raceme, the ovary is long ovate, the top of the style is curved, the stigma is capitate, the capsule is ovate, covered by the persistent calyx, the ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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