One strain of bacterium of reducing high density hexabasic chromium in alkaline medium and its cultivating method

An alkaline medium, high-concentration technology, applied in the direction of bacteria, etc., can solve problems such as chromium pollution, and achieve the effect of simple ingredients, strong adaptability, and easy large-scale cultivation

Inactive Publication Date: 2006-09-20
CENT SOUTH UNIV
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Problems solved by technology

[0005] From the perspective of bacterial metallurgy, the present invention obtains a strain of bacteria capable of efficiently reducing high-concentration Cr(VI) in alkaline media through collection, separation, and domestication, and aims at the characteristics of the strain, and provides a simple and easy-to-large-scale cultivation. The easy and low-cost cultivation method provi

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  • One strain of bacterium of reducing high density hexabasic chromium in alkaline medium and its cultivating method
  • One strain of bacterium of reducing high density hexabasic chromium in alkaline medium and its cultivating method
  • One strain of bacterium of reducing high density hexabasic chromium in alkaline medium and its cultivating method

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[0018] 1. Retrieve bacterial samples from the vicinity of the chromium slag dump site, carry out enrichment culture, and separate them on a plate after clearly obtaining effective bacterial species. Pick specific bacterial species for pure culture, and gradually increase the concentration of hexavalent chromium. Domestication, to obtain bacteria that can efficiently and rapidly reduce 2000mg / L Cr(VI).

[0019] The logarithmic phase of the bacteria was inoculated into a 10L culture solution of 920 mg / L Cr(VI), including 1.5 g / L yeast extract, 1.5 g / L sodium lactate, and 1.0 g / L sodium chloride. Under the conditions of temperature 30℃, pH11 and aerated culture, the bacteria grow and reproduce rapidly, and the growth curve is Figure 4 Shown. From Figure 4 It can be seen that the bacterial growth retardation period is short, and it quickly enters the logarithmic growth phase. The logarithmic phase is about 6 to 16 hours, and then it enters the stable phase. The number of bacteria is 1...

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Abstract

This invention provides a method for culture bacteria that can reduce high concentration Cr (VI) in basic medium. The bacteria are colorless Achromobacter sp. (CGMCC 1260) with a preservation name of Ch-1. this invention adopts nitrogen and carbon sources as the culture media, and culture at 25-25 deg.C, pH value of 7-11, and Cr (VI) concentration of 1-2000 mg/L. this invention can raise Cr (VI) reduction capacity to higher than 2g/L, and can be used in chemical plants or chromium salt treatment plants for large scale Cr (VI) reduction.

Description

technical field [0001] The present invention relates to a bacterium that reduces high-concentration hexavalent chromium in alkaline medium and its large-scale cultivation method, in particular to the biological treatment technology of alkaline chromium-containing waste water, chromium slag leachate and chromium slag from chromium salt factories and ferroalloy factories . Background technique [0002] At present, more than 100,000 tons of chromium slag are discharged every year in the country, and the accumulated stock has exceeded 4 million tons. The piled up chromium slag not only occupies a large amount of land, but also seriously pollutes groundwater due to soluble hexavalent chromium. In 2003, several serious chromium slags Pollution accidents are caused by the alkaline leachate of chromium slag. Alkaline chromium-containing wastewater or leachate of chromium slag has high pH (about 11), high concentration of Cr(VI), and high salinity. At present, there is a lack of good...

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Application Information

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IPC IPC(8): C12N1/20
Inventor 柴立元龙腾发彭兵何德文闵小波王云燕
Owner CENT SOUTH UNIV
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