Formula and preparation method of agaricus bisporus liquid spawn

A technology of liquid strain and Agaricus bisporus, which is applied in the field of bioengineering, can solve the problems of affecting the efficiency and economic effect of bisporus bisporus cultivation, unsuitable mycelium growth, aging and degeneration of mycelium, etc., so as to achieve strong mycelium vitality and fast growth speed. , anti-aging effect

Inactive Publication Date: 2013-03-13
SHANGHAI XUERONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current liquid culture medium of Agaricus bisporus has a low preparation success rate, unsuitable mycelium growth, mycelial aging and degeneration in the later stage of cultivation, and high professional technical requirements for operation. In the process of large-scale production, it will It directly affects the efficiency and economic effect of Agaricus bisporus cultivation

Method used

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  • Formula and preparation method of agaricus bisporus liquid spawn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Pour 20g of sucrose, 0.8g of magnesium sulfate, and 0.8g of potassium dihydrogen phosphate into 1000ml of aqueous solution and stir until it dissolves into a semi-finished product; then put 3g of yeast powder, 5g of potato flour, and 3g of peptone into the semi-finished product, stir thoroughly and remove Soak to make culture medium liquid. Test the pH value of the medium liquid, and adjust the pH value of the medium liquid with dipotassium hydrogen phosphate to keep the pH value at 6.5-7.0.

[0032] Put the medium liquid into the sterilization equipment for sterilization at 121-123° C. and high-temperature sterilization at 1.3 standard atmospheric pressure. During high-temperature sterilization, keep the high-temperature sterilization state for at least 15 minutes. Then, the medium liquid at high temperature is stirred at a stirring speed of 150-200rpm under aseptic conditions, and then the medium liquid is cooled to keep the temperature of the medium liquid at 20-21.9...

Embodiment 2

[0034] Pour 15g of sucrose, 10g of maltose, 0.8g of magnesium sulfate, 0.8g of potassium dihydrogen phosphate, 3g of yeast powder, and 3g of peptone into 1000ml of aqueous solution, and fully stir with a mixer before defoaming.

[0035] Use pH test paper to test the pH value of the medium liquid, and use dipotassium hydrogen phosphate and potassium dihydrogen phosphate to adjust the pH value of the medium liquid to be neutral. Then put the medium liquid into the sterilization equipment and carry out high-temperature and high-pressure sterilization for 30 minutes. During the sterilization, the temperature of the sterilization is adjusted to 124-125° C., and the air pressure is 1.3 standard atmospheres.

[0036] After the sterilization is completed, the culture medium liquid is taken out under aseptic condition and cooled to 22-23.9° C., followed by inoculation and aerobic cultivation as in the above-mentioned Example 1.

Embodiment 3

[0038] Pour 25g of glucose, 0.8g of magnesium sulfate, and 0.8g of potassium dihydrogen phosphate into 1000 ml of aqueous solution, and stir to dissolve it, then add 3g of yeast powder, 5g of potato flour, 3g of peptone, and 3g of soybean peptide, and pass through the mixer Thoroughly stir to become a medium liquid, and defoam the medium liquid. And use a pH tester to test the pH value of the medium liquid, and use hydrochloric acid and sodium hydroxide to adjust the pH value of the medium liquid to 6.5-7.0.

[0039]Then put the medium liquid into the ultrasonic sterilizer for sterilization for at least 30 minutes, then cool the medium liquid to 24-25°C, insert Agaricus bisporus strains, and purify the culture medium liquid containing Agaricus bisporus strains Air is used for aerobic cultivation for 7-10 days. During aerobic cultivation, the temperature in the cultivation chamber is kept at 20-23° C., and the medium liquid containing Agaricus bisporus species is shaken and cul...

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Abstract

The invention relates to the field of bioengineering, in particular to a culture medium and a preparation method thereof. According to a formula, an agaricus bisporus liquid spawn includes raw materials and a water solution. The raw materials are contained in the water solution and are composed of the following components by weight: 0.1-1.0% of yeast powder, 1.5-3% of sugar, 0.05-0.3% of potassium dihydrogen phosphate, 0.05-0.3% of magnesium sulfate, and 0.1-0.5% of peptone. The preparation method of the agaricus bisporus liquid spawn comprises the steps of: culture medium preparation, culture medium adjustment, culture medium sterilization, and mycelium inoculation. By adopting the technology, the agaricus bisporus mycelia cultivated by the invention have the advantages of vigorous vitality, difficult aging, rapid spawn running speed, and strong disease resistance, thus being easy for large-scale planting.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a culture medium and a preparation method thereof. Background technique [0002] Edible fungus is a food with high nutritional value that people like. In recent years, the artificial cultivation technology of edible fungi has made great progress. Edible mushrooms include Pleurotus eryngii, shiitake mushrooms, and Agaricus bisporus. Among them, Agaricus bisporus has plump meat, delicious taste, and thick taste, and is widely favored by people. In the process of cultivating Agaricus bisporus, many links will be involved, and the mycelium cultivation of Agaricus bisporus is one of the important links in the cultivation of Agaricus bisporus. [0003] In the production process of Agaricus bisporus, the strains used in the production of Agaricus bisporus include solid strains and liquid strains. Compared with solid strains, liquid strains have the advantages of shorter cultivation time,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 胡卫红
Owner SHANGHAI XUERONG BIOTECH
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