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Rapid propagation method for smilax glabra roxb tissue culture

A technology of smilax miltiorrhiza and tissue culture, applied in the field of plants, can solve problems such as low development and utilization rate, and achieve the effects of large yield, low energy consumption, and large-scale planting.

Inactive Publication Date: 2014-12-24
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The leaves contain quercetin and kaempferol, slightly sweet in taste, flat in nature, clearing heat and dampness, reducing swelling and dissipating stagnation, strengthening muscles and bones, treating diarrhea, irregular menstruation, rheumatic arthralgia, gonorrhea, white turbidity, leucorrhea, and scrofula At present, its utilization is mainly collected in the wild, and the development and utilization rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the young leaves of Smilax glabra, wash them with running water for 15 minutes, disinfect them with sodium hypochlorite on the ultra-clean workbench for 9 minutes, wash them with sterile water 5 times, put the sterilized young leaves of Smilax glabra in a -1°C refrigerator Low-temperature pretreatment in medium and then inserting ER+IBA0.8mg / L+ZT3mg / L+S-33070.3mg / L+0.7% agar+30g / L sucrose medium for callus induction, pH5.85, Culture in dark light, temperature 26±2℃, put the induced callus into the medium ER+IBA0.5mg / L+ZT0.8mg / L+0.02mg / LTDZ for callus differentiation culture, add sucrose 30g / L , agar 7g / L, pH5.85, light 2700lx, temperature 26±2℃, the differentiated sprouts were inserted into 1 / 4MS+S-33070.7mg / L medium for rooting induction, added sucrose 25g / L, agar 7g / L, pH 5.85, light intensity 10000lx, light time 16h / d, temperature 26±2°C, when the test tube seedlings are about 5cm long and the root length is about 2cm, put them into the substrate paved with high...

Embodiment 2

[0011] Take the young leaves of Smilax glabra, wash them with running water for 15 minutes, disinfect them with sodium hypochlorite on the ultra-clean workbench for 9 minutes, wash them with sterile water 5 times, put the sterilized young leaves of Smilax glabra in a -1°C refrigerator Low-temperature pretreatment in medium and then inserting ER+IBA0.8mg / L+ZT3mg / L+S-33070.5mg / L+0.7% agar+30g / L sucrose medium for callus induction, pH5.85, Culture in dark light, temperature 26±2℃, put the induced callus into the medium ER+IBA0.5mg / L+ZT0.8mg / L+0.02mg / LTDZ for callus differentiation culture, add sucrose 30g / L , agar 7g / L, pH5.85, light 2700lx, temperature 26±2°C, the differentiated sprouts were inserted into 1 / 4MS+S-33070.9mg / L medium for rooting induction, additional sucrose 25g / L, agar 7g / L, pH 5.85, light intensity 10000lx, light time 16h / d, temperature 26±2°C, when the test tube seedlings are about 5cm long and the root length is about 2cm, put them into the substrate paved wit...

Embodiment 3

[0013] Take the young leaves of Smilax glabra, wash them with running water for 15 minutes, disinfect them with sodium hypochlorite on the ultra-clean workbench for 9 minutes, wash them with sterile water 5 times, put the sterilized young leaves of Smilax glabra in a -1°C refrigerator Low-temperature pretreatment in medium and then inserting ER+IBA0.8mg / L+ZT3mg / L+S-33070.4mg / L+0.7% agar+30g / L sucrose medium for callus induction, pH5.85, Culture in dark light, temperature 26±2℃, put the induced callus into the medium ER+IBA0.5mg / L+ZT0.8mg / L+0.02mg / LTDZ for callus differentiation culture, add sucrose 30g / L , agar 7g / L, pH5.85, light 2700lx, temperature 26±2°C, the differentiated sprouts were inserted into 1 / 4MS+S-33070.8mg / L medium for rooting induction, additional sucrose 25g / L, agar 7g / L, pH 5.85, light intensity 10000lx, light time 16h / d, temperature 26±2°C, when the test tube seedlings are about 5cm long and the root length is about 2cm, put them into the substrate paved wit...

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PUM

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Abstract

The invention discloses a rapid propagation method for smilax glabra roxb tissue culture. The rapid propagation method for smilax glabra roxb tissue culture comprises the steps of obtaining smilax glabra roxb sterile blades, inducing callus, differentiating callus, inducing rooting, acclimatizing and transplanting, and the like. With the adoption of the tissue culture method, smilax glabra roxb seedlings can be obtained in a large batch, the production is controllable, the quality is excellent, and a basis is provided for development and utilization of smilax glabra roxb.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of Smilax glabra, belonging to the technical field of plants. Background technique [0002] Smilax glabra, Smilax corbularia Kunth , Liliaceae, climbing shrubs, produced in Gansu (south) and the provinces south of the Yangtze River Basin, until Taiwan, Hainan Island and Yunnan, also distributed in Vietnam, Thailand and India, born in forests, shrubs, and shrubs below 1800 meters above sea level. On river banks or valleys, also in forest margins and sparse forests. The rhizomes contain astilbin, jasmin, 3-O-caffeoylshikimic acid, shikimic acid, ferulic acid, β-sitosterol, and glucose. The leaves contain quercetin and kaempferol, slightly sweet in taste, flat in nature, clearing heat and dampness, reducing swelling and dissipating stagnation, strengthening muscles and bones, treating diarrhea, irregular menstruation, rheumatic arthralgia, gonorrhea, white turbidity, leucorrhea, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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