Strain in use sensor of measuring oxygen quantity needed by industrial wastewater or seawater biochemistry-method for cultivating bacillus licheniformis

A technology of Bacillus licheniformis and biochemical oxygen demand, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of inconsistent microbial properties, few types of industrial wastewater, and unstable measurement In order to achieve the effect of large measurement response signal, strong vitality and improved strain performance

Inactive Publication Date: 2005-01-12
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0004] Biosensors, which usually consist of complex bacteria, have a high generality of organic matter, but the inconsistency of microbial properties leads to instability in measurement
However, the use of a single strain has good measurement stability, and the general substrate is poor in generality, and there are few types of industrial wastewater suitable for measurement.
[0005] After searching, so far no strains have been found for seawater biochemical oxygen demand sensor measurement

Method used

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  • Strain in use sensor of measuring oxygen quantity needed by industrial wastewater or seawater biochemistry-method for cultivating bacillus licheniformis
  • Strain in use sensor of measuring oxygen quantity needed by industrial wastewater or seawater biochemistry-method for cultivating bacillus licheniformis
  • Strain in use sensor of measuring oxygen quantity needed by industrial wastewater or seawater biochemistry-method for cultivating bacillus licheniformis

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Effect test

Embodiment 1

[0051] Example 1: A method for cultivating Bacillus licheniformis strains used in coking plant wastewater BOD sensor measurement:

[0052] (1) Screen and isolate Bacillus licheniformis from starch factory production wastewater:

[0053] a. Prepare solid medium according to the following components:

[0054] 0.5 grams of beef extract, 0.5 grams of peptone, 0.5 grams of soluble starch, 0.1 grams of magnesium chloride, 1.5 grams of agar, 100 milliliters of starch factory wastewater; adjust the pH value to 6.8-7.2, and autoclave at 121 ° C for 25 minutes;

[0055] b. Use waste water produced by a starch factory as a source of bacteria and evenly spread it on a solid plate, and cultivate it in an incubator at 28-32°C; Carry out expansion culture in the liquid medium without adding agar of the component a, the rotating speed of the shaker is 130-160 rpm, the culture temperature is 28-32 °C, after the bacteria grow out, transfer 2-3 generations continuously, 12-24 hours per generat...

Embodiment 2

[0061] Embodiment 2: a kind of culture method that is used for seawater BOD sensor to measure bacterial strain-Bacillus licheniformis:

[0062] (1) Screen and isolate Bacillus licheniformis from starch factory production wastewater:

[0063] a. Prepare solid medium according to the following components:

[0064] 0.5 grams of beef extract, 0.5 grams of peptone, 0.5 grams of soluble starch, 0.1 grams of magnesium chloride, 1.5 grams of agar, 100 milliliters of starch factory wastewater; adjust the pH value to 6.8-7.2, and autoclave at 121 ° C for 25 minutes;

[0065] b. Use waste water produced by a starch factory as a source of bacteria and evenly spread it on a solid plate, and cultivate it in an incubator at 28-32°C; Carry out expansion culture in the liquid culture medium without adding agar of the component a, the shaker speed is 130-160 rpm, and the culture temperature is 28-32°C. After the bacteria grow out, transfer 2-3 generations continuously. , 12-24 hours per gener...

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Abstract

A method for cultivating Bacillus licheniformis being applied in BOD biological transducer includes separating and screening waste water from starch plant for obtaining a high breath-strength aerobic bacterial strain, acclimating the bacterial strain to be operational bacterial strain set in BOD biological transducer for in dustrial waste water or seawater.

Description

technical field [0001] The invention relates to a method for cultivating bacillus licheniformis, which is used for industrial wastewater or seawater biochemical oxygen demand sensors to measure bacterial strains. technical background [0002] The "biochemical oxygen demand (BOD)" of a water body is one of the main indicators to measure whether the water quality is polluted by organic matter and the degree of pollution. At present, the methods for measuring this index at home and abroad all follow the monitoring method of five-day cultivation. This method is complicated to operate, highly disturbing, poor in reproducibility, time-consuming and labor-intensive. Microbial sensor measurement of BOD has become a research hotspot in the past twenty years because of its advantages of fast, accurate and easy to realize continuous online automatic monitoring. The BOD microbial sensor is mainly composed of immobilized microbial film, transducer and signal outpu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20
Inventor 崔建升王晓晖马莉陈曦
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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