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Method for extracting and measuring ABA (abscisic acid) of strawberry

A strawberry and extract technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of less reagent consumption, accurate and reliable results, and improved extraction efficiency

Active Publication Date: 2014-12-10
建瓯市梦园农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the analysis of strawberry ABA by UPLC

Method used

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  • Method for extracting and measuring ABA (abscisic acid) of strawberry
  • Method for extracting and measuring ABA (abscisic acid) of strawberry
  • Method for extracting and measuring ABA (abscisic acid) of strawberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of standard solution: take 25mg of ABA reference substance, add 10mL of chromatographically pure methanol to dissolve and constant volume, to make a standard solution mother solution with a concentration of 2.5mg / mL (it should be noted that when the mother solution is not When used immediately, it needs to be stored at -20°C), and then the mother solution was diluted into 7 concentration gradients of 0.1, 0.25, 0.5, 0.75, 1, 2.5, and 5 μg / mL;

[0028] 2. Preparation of extract: Weigh 5g of strawberry green fruit pulp, grind it into powder with liquid nitrogen, then place it in a stoppered Erlenmeyer flask, and add 200mL of extraction solvent (methanol, ethyl acetate, formic acid at 50:50 : 1 volume ratio), after vigorously shaking and extracting for 5 minutes, the residue was discarded by suction filtration, and the obtained filtrate was rotary evaporated to dryness under reduced pressure at 35°C, and then the mobile phase of ultra-high performance liquid ...

Embodiment 2

[0041] Preparation of extract: Weigh 10g of strawberry red fruit pulp, grind it into powder with liquid nitrogen, then put it into a stoppered Erlenmeyer flask, and add 400mL of extraction solvent (methanol, ethyl acetate, formic acid at 50:50:1 mixed by volume ratio), shake vigorously to extract for 5 minutes, remove the residue by suction filtration, place the obtained filtrate at 35°C and rotary evaporate to dryness, then add the mobile phase of ultra-high performance liquid chromatography to dissolve, and collect the solution to obtain Strawberry ABA extract, the resulting extract is passed through a 0.22 μm filter membrane to obtain the test solution;

[0042] Determination: Take the liquid to be tested prepared in this embodiment and inject it into an ultra-high performance liquid chromatograph with the same chromatographic conditions as in Example 1 for analysis and determination, and obtain the ultra-high performance liquid chromatogram of the liquid to be tested, speci...

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Abstract

The invention provides a method for extracting and measuring ABA of a strawberry. The method comprises the steps as follows: a mixed liquid of methyl alcohol, ethyl acetate and formic acid in a volume ratio of 50: 50:1 is taken as an extraction solvent, an ABA component in strawberry fruit pulp can be extracted in shorter time, residue is removed through suction filtration, an obtained filter liquor is decompressed and rotationally steamed to be dried at the temperature of 35 DEG C, and then, a flowing phase of an ultra-high performance liquid chromatography is added for dissolution, a solution is collected, and an extracting solution of strawberry ABA is obtained after filtered by a 0.22 mu m filter membrane; and the obtained extracting solution is injected into an ultra-high performance liquid chromatographic instrument under the set chromatographic condition for measurement and analysis. The method has the characteristics of high extraction speed, measurement accuracy and sensitivity and good repeatability, further, a measurement result is accurate and reliable, and the dosage of a reagent is small, so that the measurement speed is greatly increased, and the solvent consumption is reduced.

Description

【Technical field】 [0001] The invention relates to a method for extracting and measuring components in plants, in particular to a method for extracting and measuring ABA in strawberries. 【Background technique】 [0002] Abscisic acid (ABA) is one of the important endogenous hormones that regulate plant growth and widely exists in higher plants. Abscisic acid can inhibit plant cell division and seed germination, promote bud and seed dormancy, and promote senescence and shedding of leaves and fruits. Strawberry (Fragaria ananassa Duch) is a typical non-climacteric fruit, and its ripening regulation and post-harvest freshness preservation have always been a difficult problem in the process of production, transportation and sales. Whether ABA plays a key role in the regulation of strawberry ripening is a hotspot in current research. Accurate determination of ABA content in strawberry and study of its change rule are of great significance for exploring fruit ripening and storage ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 朱海生马宏棋温庆放
Owner 建瓯市梦园农业发展有限公司
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