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Rapid propagation method for asparagus fern mutagenesis breeding

A fast technology for mutation breeding, applied in the field of plant cultivation, can solve the problems that tissue culture methods have not been reported yet, and achieve the effects of large-scale planting, large yield and high regeneration rate

Inactive Publication Date: 2015-01-28
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, seeds and divisions are mainly used for propagation, and the tissue culture method has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Select plump seeds of Asparagus, soak them in 70% alcohol for 25 minutes, disinfect them with 1% sodium hypochlorite solution for 18 minutes, rinse them with sterile water, and inoculate the sterilized Asparagus seeds into the modified MS culture without any hormones. Add 45g / L sucrose, 6.5g / L agar, pH6.0, temperature 25℃, nitrous acid treatment for 10min, cut off the radicle and inoculate into MT+3.0mg / L6-BA+ 0.5mg / L2, 4-D medium for cluster bud proliferation culture, add sucrose 30g / L, agar 6.5g / L, pH6.0, temperature 25℃, light intensity 35μmol m -2 ·s -1 , light time 10h / d, three clusters of proliferated buds were inoculated into rooting medium of MT+NAA0.4mg / L+activated carbon 3g / L for rooting induction, adding sucrose 20g / L, agar 5.5g / L, pH6.0, temperature 27℃, light intensity 65μmol·m -2 ·s -1 , the light time is 17h / d, and the mutagenic activity rate is 91%.

Embodiment 2

[0011] Select plump seeds of Asparagus, soak them in 70% alcohol for 25 minutes, disinfect them with 1% sodium hypochlorite solution for 18 minutes, rinse them with sterile water, and inoculate the sterilized Asparagus seeds into the modified MS culture without any hormones. Add 45g / L sucrose, 6.5g / L agar, pH6.0, temperature 25℃, nitrous acid treatment for 20min, cut off the radicle and inoculate into MT+3.0mg / L6-BA+ 0.5mg / L2, 4-D medium for cluster bud proliferation culture, add sucrose 30g / L, agar 6.5g / L, pH6.0, temperature 25℃, light intensity 35μmol m -2 ·s -1 , light time 10h / d, three clusters of proliferated buds were inoculated into rooting medium of MT+NAA0.4mg / L+activated carbon 3g / L for rooting induction, adding sucrose 20g / L, agar 5.5g / L, pH6.0, temperature 27℃, light intensity 65μmol·m -2 ·s -1 , the light time is 17h / d, and the mutagenic activity rate is 92%.

Embodiment 3

[0013] Select plump seeds of Asparagus, soak them in 70% alcohol for 25 minutes, disinfect them with 1% sodium hypochlorite solution for 18 minutes, rinse them with sterile water, and inoculate the sterilized Asparagus seeds into the modified MS culture without any hormones. Add 45g / L sucrose, 6.5g / L agar, pH6.0, temperature 25℃, nitrous acid treatment for 30min, cut off the radicle and inoculate into MT+3.0mg / L6-BA+ 0.5mg / L2, 4-D medium for cluster bud proliferation culture, add sucrose 30g / L, agar 6.5g / L, pH6.0, temperature 25℃, light intensity 35μmol m -2 ·s -1 , the light time is 10h / d, and the proliferated clusters of shoots are inoculated into the rooting medium of MT+NAA0.4mg / L+activated carbon 3g / L for rooting induction, adding 20g / L of sucrose and 5.5g / L of agar, pH6.0, temperature 27℃, light intensity 65μmol·m -2 ·s -1 , the light time is 17h / d, and the mutagenic activity rate is 94%.

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Abstract

The invention discloses a rapid propagation method for asparagus fern mutagenesis breeding. The rapid propagation method comprises the following steps: seed disinfection, seed mutagenesis, cluster bud proliferation, rooting induction and the like. According to the method disclosed by the invention, metabolic pathways of cells are changed by processing through a mutagenic reagent so as to obtain high-yield strains with genetic stability.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of asparagus, belonging to the technical field of plant cultivation. Background technique [0002] Asparagus, Liliaceae, climbing perennial herb. Born in damp mountain forest edge, grass or bushes, also cultivated. Distributed in East China, Central South, Southwest and Hebei, Shanxi, Shaanxi, Gansu, Taiwan and other places. Cold in nature, sweet in taste, slightly bitter. Contains a variety of spirosteroid compounds aspartoside Ⅳ~Ⅶ, nearly 20 kinds of amino acids such as asparagine, citrulline, serine, and oligosaccharides Ⅰ~Ⅶ; and contains 5-methoxy-methylfurfural, It has the effects of nourishing yin and clearing away heat, nourishing the lungs and nourishing the kidneys. It is used for fever due to yin deficiency, lung abscess, thirst, increasing blood cells, enhancing phagocytosis of the reticuloendothelial system and prolonging the existence time of antibodies. At present...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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