Chemical disinfection tissue culture method for miscanthus giganteus

Abstract

The invention relates to a chemical disinfection tissue culture method for miscanthus giganteus. The method comprises the following steps: culturing container disinfection, medium preparation, callus induction culturing, differentiation culturing, rooting culturing, and bottle seedling transplanting. According to the method, chemical reagents are used for achieving the aim of bacteria killing or bacteria inhibition; in the process of preparing culture media, culture containers and the culture media do not need to be subjected to high-temperature and high-pressure disinfection, so that the work amount and the energy consumption can be reduced, and the step of miscanthus giganteus tissue culture is simplified; the operation is easy as the media are prepared according to different media formulas; the practicability is high, the generalization performance is high, and the tissue culture cost of miscanthus giganteus can be effectively reduced and can be reduced by 10% or more generally.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a giant awn chemical disinfection tissue culture method, which belongs to the field of biotechnology. Background technique [0002] Miscanthus giganteus, also known as Miscanthus giganteus, is a perennial C4 grass belonging to the genus Miscanthus giganteus. The plant height can reach 4m, so it is called giant awn. Originally produced in Japan, it was introduced to Denmark in the 1940s, and was later introduced by many European countries and the United States as an energy plant for research and planting. In recent years, many domestic units, including China Agricultural University and the Chinese Academy of Sciences, have successively introduced plants as energy sources for research. The reason why giant awn is considered as the most potential energy crop in Europe and the United States, and is endowed with the star of hope for solving future energy and environmental problems. It...

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Problems solved by technology

All aspects of tissue culture need to be equipped with corresponding facilities, equipment and conventional consumables, which is costly and consumes a lot of energy. At the same time, the entire process must be operated under sterile conditions, and the cost of manual operation is high.

If the culture medium can be modified to obtain a medium that not only ensures the normal growth of tissue culture seedlings, but also has bactericidal, antibacterial or bacteriostatic functions, this can greatly save production costs such as tissue culture seedlings, and is a solution to the production of giant awn seedlings. The problem of high application cost

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