Chemical disinfection tissue culture method for Saccharum officimarum.L

Abstract

The invention relates to a chemical disinfection tissue culture method for Saccharum officimarum.L. The method comprises the following steps: culturing container disinfection, medium preparation, seed stem treatment and induction culturing, multiplication culturing, seedling hardening culturing, rooting culturing, and bottle seedling transplanting. According to the method, chemical reagents are used for achieving the aim of fungi killing or fungi inhibition; in the process of preparing culture media, culture containers and the culture media do not need to be subjected to high-temperature and high-pressure disinfection, so that the work amount and the energy consumption can be reduced, and the step of chewing cane tissue culture is simplified; the operation is easy as the media are prepared according to different media formulas; the practicability is high, the generalization performance is high, and the chewing cane tissue culture cost can be effectively reduced and can be reduced by 10% or more generally.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a fruit cane chemical disinfection tissue culture method, which belongs to the field of biotechnology. Background technique [0002] Fruit cane (Saccharum officimarum. L) is a type of tropical species of Saccharum with large stems, thin skin, crisp texture, juicy and sweet taste. With its unique flavor, rich nutrition and practical medicinal value, fruit cane has become more and more popular fruit. my country is rich in fruit cane resources. According to the color of the cane stem, it can be divided into yellow-skinned fruit cane, black-skinned fruit cane, white-skinned fruit cane and green-skinned fruit cane. Among them, the black-skinned fruit cane grown in Guangdong and Guangxi is the most famous. [0003] In recent years, the planting area of ​​fruit cane has been increasing, but the production also faces two key problems: one is that the fruit cane is infected by various dis...

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Problems solved by technology

[0003] In recent years, the planting area of ​​fruit cane has been increasing, but the production also faces two key problems: one is that the fruit cane is infected by various diseases and insect pests, and the fruit cane stems on the market usually have mosaic disease , Perennial dwarf disease and other diseases, causing variety degeneration, leading to shortening of cane stems, shortening of internodes, decline in yield, and deterioration of quality; second, fruit cane is an asexually propagated crop with a small reproduction coefficient, which is not conducive to the rapid promotion of improved varieties

Conventional fruit cane tissue culture methods require the inoculation process and the culture process to be carried out in sterile medium, which consumes a lot of energy for aseptic disinfection and high manual operation costs

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