Chemical disinfection tissue culture method for rhododendron simsii planch

A chemical disinfection and rhododendron technology, applied in the biological field, can solve the problems of high energy consumption and high cost of manual operation, and achieve the effect of simplifying tissue culture procedures, reducing workload and energy consumption, and simple operation

Inactive Publication Date: 2015-09-09
FUJIAN AGRI & FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

The conventional rhododendron tissue culture method requires the explants to be inoculated in the sterile medium after high temperature and high pressure sterilization to grow normally, which consumes a lot of energy and manual operation.

Method used

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  • Chemical disinfection tissue culture method for rhododendron simsii planch
  • Chemical disinfection tissue culture method for rhododendron simsii planch
  • Chemical disinfection tissue culture method for rhododendron simsii planch

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Embodiment 1

[0034] Embodiment 1: A kind of Rhododendron chemical disinfection tissue culture method

[0035] A rhododendron chemical disinfection tissue culture method, comprising the following steps:

[0036] 1. Disinfection of the culture container: Soak the cap of the culture bottle and the stainless steel plate for inoculation in an aqueous solution of 100 mg / L sodium hypochlorite + 2 mg / L penicillin + 100 mg / L calcium propionate for 10 hours, and save it for later use.

[0037]2. Medium preparation: induction medium is ER+10mg / L ZT+10mg / L IAA+80mg / L sodium hypochlorite+3mg / L penicillin+50mg / L calcium propionate+20g / L sucrose+3.0g / L agar powder , pH5.8; proliferation medium: ER+1mg / L ZT+1.0mg / L IAA+80mg / L sodium hypochlorite+2mg / L penicillin+50mg / L calcium propionate+20g / L sucrose+3.0g / L agar powder, pH5.8; rooting medium is 1 / 2ER+1.0mg / L NAA+0.5IBA mg / L+80mg / L sodium hypochlorite+2mg / L penicillin+80mg / L calcium propionate+20g / L sucrose+3.0g / L agar powder, pH5.8; when preparing the...

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Abstract

The invention relates to a chemical disinfection tissue culture method for rhododendron simsii planch. The chemical disinfection tissue culture method for rhododendron simsii planch comprises the steps of culture vessel disinfection, culture medium preparation, induction culture, enrichment culture, rooting culture and bottle seedling transplanting. According to the chemical disinfection tissue culture method for rhododendron simsii planch, the purpose of disinfection or bacteriostasis can be achieved by a chemical reagent; in the culture medium preparation process, a culture vessel and a culture medium do not need to be disinfected at high temperature and high pressure, the workload and the energy consumption can be reduced, and the rhododendron simsii planch tissue culture link can be simplified; the operation is simple, and the preparation is implemented according to different culture medium formulae; the practicability is high, the popularization is good, the tissue culture cost of rhododendron simsii planch can be effectively lowered, and the cost can be generally reduced by no less than 10%.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a rhododendron chemical disinfection tissue culture method, which belongs to the field of biotechnology. Background technique [0002] Rhododendron Linn. is a world-renowned ornamental plant, one of the top ten famous flowers in my country, and also a world-famous ornamental flower. Its bright and colorful colors and decent potted flower shapes have always occupied one of the best-selling varieties of flowers during the Spring Festival in my country. . Rhododendron is usually propagated by seeds, cuttings, layering and grafting, but due to limitations of species or varieties, seasons, mother plant materials and other factors, it is difficult to carry out large-scale commercial production. Since Anderon successfully established the in vitro culture system of Rhododendron xx for the first time in 1975, tissue culture has become an important means of Rhododendron xx propagation, wide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郭晋隆林庆良许莉萍高世武阙友雄
Owner FUJIAN AGRI & FORESTRY UNIV
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