Cultivation method for ganoderma lucidum
A cultivation method and technology of Ganoderma lucidum, applied in the directions of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems of harsh growth conditions and lack of cultivation methods, and achieve the effect of simple cultivation method, unique method and cost reduction.
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Embodiment 1
[0031] Embodiment 1: a kind of breeding method of blood ganoderma, comprises the steps:
[0032] (1) Incline cultivation
[0033] ①Slant culture medium formula: by weight percentage, it is prepared from 16% of potato, 1.6% of glucose, 1.6% of agar and the balance of acacia tree water extract;
[0034] ②Preparation of slant medium: Weigh the potatoes that have been washed, peeled and cut into small pieces in proportion, add the acacia wood infusion in proportion, boil and filter to obtain potato juice; add agar in proportion to the potato juice and glucose, boiled and dissolved, and filtered to obtain a slant medium solution, which was packed into test tubes and sterilized at high temperature and high pressure to obtain a test tube slant medium;
[0035] ③Cultivation of slant strains: inoculate the parent species of Ganoderma lucidum on the slant medium of the test tube, and cultivate it for 7 days at a temperature of 27°C. After the mycelium grows all over the slant, select t...
Embodiment 2
[0048] Embodiment 2: a kind of breeding method of blood ganoderma, comprises the steps:
[0049] (1) Incline cultivation
[0050] ①Slant culture medium formula: by weight percentage, it is prepared from 13% of potato, 1.8% of glucose, 1.5% of agar and the balance of acacia tree water extract;
[0051] ②Preparation of slant medium: Weigh the potatoes that have been washed, peeled and cut into small pieces in proportion, add the acacia wood infusion in proportion, boil and filter to obtain potato juice; add agar in proportion to the potato juice and glucose, boiled and dissolved, and filtered to obtain a slant medium solution, which was packed into test tubes and sterilized at high temperature and high pressure to obtain a test tube slant medium;
[0052] ③Cultivation of slant strains: Inoculate the parent species of Ganoderma lucidum on the slant medium of the test tube, and cultivate it for 8 days at a temperature of 26°C. After the mycelium grows all over the slant, select t...
Embodiment 3
[0065] Embodiment 3: a kind of breeding method of blood ganoderma, comprises the steps:
[0066] (1) Incline cultivation
[0067] ①Slant medium formula: by weight percentage, it is prepared from 18% of potato, 1.3% of glucose, 1.7% of agar and the balance of acacia tree water extract;
[0068] ②Preparation of slant medium: Weigh the potatoes that have been washed, peeled and cut into small pieces in proportion, add the acacia wood infusion in proportion, boil and filter to obtain potato juice; add agar in proportion to the potato juice and glucose, boiled and dissolved, and filtered to obtain a slant medium solution, which was packed into test tubes and sterilized at high temperature and high pressure to obtain a test tube slant medium;
[0069] ③Cultivation of slant strains: Inoculate the parent species of Ganoderma lucidum on the slant medium of the test tube, and culture it for 6 days at a temperature of 28°C. After the mycelium grows all over the slant, select the slant m...
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