56 results about "Ricinus sanguineus" patented technology

The present invention is directed to protocells for specific targeting of hepatocellular and other cancer cells which comprise a nanoporous silica core with a supported lipid bilayer; at least one agent which facilitates cancer cell death (such as a traditional small molecule, a macromolecular cargo (e.g. siRNA or a protein toxin such as ricin toxin A-chain or diphtheria toxin A-chain) and / or a histone-packaged plasmid DNA disposed within the nanoporous silica core (preferably supercoiled in order to more efficiently package the DNA into protocells) which is optionally modified with a nuclear localization sequence to assist in localizing protocells within the nucleus of the cancer cell and the ability to express peptides involved in therapy (apoptosis / cell death) of the cancer cell or as a reporter, a targeting peptide which targets cancer cells in tissue to be treated such that binding of the protocell to the targeted cells is specific and enhanced and a fusogenic peptide that promotes endosomal escape of protocells and encapsulated DNA. Protocells according to the present invention may be used to treat cancer, especially including hepatocellular (liver) cancer using novel binding peptides (c-MET peptides) which selectively bind to hepatocellular tissue or to function in diagnosis of cancer, including cancer treatment and drug discovery.

The disclosure provides compositions relating to thermostable vaccines and methods of preparing same. Specifically, the disclosure provides for methods of preparing thermostable vaccines based on a recombinant ricin neurotoxin protein and uses of co-adjuvants to develop a composition capable of eliciting an immune response in a subject.

The present invention is directed to protocells for specific targeting of hepatocellular and other cancer cells which comprise a nanoporous silica core with a supported lipid bilayer; at least one agent which facilitates cancer cell death (such as a traditional small molecule, a macromolecular cargo (e.g. siRNA or a protein toxin such as ricin toxin A-chain or diphtheria toxin A-chain) and / or a histone-packaged plasmid DNA disposed within the nanoporous silica core (preferably supercoiled in order to more efficiently package the DNA into protocells) which is optionally modified with a nuclear localization sequence to assist in localizing protocells within the nucleus of the cancer cell and the ability to express peptides involved in therapy (apoptosis / cell death) of the cancer cell or as a reporter, a targeting peptide which targets cancer cells in tissue to be treated such that binding of the protocell to the targeted cells is specific and enhanced and a fusogenic peptide that promotes endosomal escape of protocells and encapsulated DNA. Protocells according to the present invention may be used to treat cancer, especially including hepatocellular (liver) cancer using novel binding peptides (c-MET peptides) which selectively bind to hepatocellular tissue or to function in diagnosis of cancer, including cancer treatment and drug discovery.

The present invention is directed to protocells for specific targeting of hepatocellular and other cancer cells which comprise a nanoporous silica core with a supported lipid bilayer; at least one agent which facilitates cancer cell death (such as a traditional small molecule, a macromolecular cargo (e.g. siRNA or a protein toxin such as ricin toxin A-chain or diphtheria toxin A-chain) and / or a histone-packaged plasmid DNA disposed within the nanoporous silica core (preferably supercoiled in order to more efficiently package the DNA into protocells) which is optionally modified with a nuclear localization sequence to assist in localizing protocells within the nucleus of the cancer cell and the ability to express peptides involved in therapy (apoptosis / cell death) of the cancer cell or as a reporter, a targeting peptide which targets cancer cells in tissue to be treated such that binding of the protocell to the targeted cells is specific and enhanced and a fusogenic peptide that promotes endosomal escape of protocells and encapsulated DNA. Protocells according to the present invention may be used to treat cancer, especially including hepatocellular (liver) cancer using novel binding peptides (c-MET peptides) which selectively bind to hepatocellular tissue or to function in diagnosis of cancer, including cancer treatment and drug discovery.

The invention relates to a method for reinforcing a plant and microorganism symbiosis system through bio-surfactant and application of the system to in-situ repair of uranium polluted soil. Uranium-pollution-free soil, vermiculite and peat soil serve as culture media, ricinus communis serves as a soil host plant, arbuscular mycorrhizae fungi serve as inoculation microorganisms, at first, a seedling culture device is utilized for culturing ricinus communis seedlings, ricinus communis seeds are inoculated with the arbuscular mycorrhizae fungi, a ricinus communis seedling-mycorrhizae fungus symbiosis system is formed, then bio-surfactant strain liquid is used for reinforcing the ricinus communis seedling-mycorrhizae fungus symbiosis system, and the ricinus communis seedling-mycorrhizae fungus symbiosis system with the bio-surfactant reinforcing function is obtained. The system is applied to in-situ repair of the uranium polluted soil. In the process of in-situ repair of the uranium polluted soil, the plant grows well, moreover, after the soil is repaired, the content of uranium in the plant is high, and the repair effect is good.

Compositions and methods for treatment of toxin poisoning are disclosed.

The present invention is directed to methods for detecting the presence of ricin and compounds that catalyze the release of adenine from nucleic acids or other biological materials and for screening for inhibitors of such compounds.

The invention relates to a new polynucleotide which encodes a polypeptide expressed in the salivary glands of ticks, more particularly the Ixodes ricinus arthropod tick, during the slow-feeding phase of the blood meal have, said polynucleotide and related polypeptide may be used in different constructions and for different applications which are also included in the present invention.

An odor absorbing composition is provided comprising zinc salt of ricinoleic acid, a solubility promoter including sodium iminodisuccinate, water and optionally, other ingredients such as perfumes and antifungal agents or bactericides. The zinc ricinoleate can be completely solubilized in water, yet the solution will exhibit low foaming and friability. In addition, the end product may be in the form of a sprayable liquid, a thick liquid capable of clinging to a vertical surface, a gel or solid tablet, a powder, or any other form. The present invention further relates to a method for forming an odor absorbing composition comprising zinc salt of ricinoleate acid, sodium iminodisuccinate and water.

The present invention relates to the generation of stable haploid cell cultures, uses of said cells in forward and reverse genetics, especially the identification of target genes associated with a modified phenotype and in particular identifying genetic targets associated with toxin resistance, especially ricin toxicity resistance, and therapeutic uses of target compounds.

The invention provides a method for producing a medium-density fiberboard through branches and trunk of ricinus communis and stalks of miscanthus. The method comprises the following steps: 1, after the branches and trunk of ricinus communis and stalks of miscanthus are mixed, cutting the mixture into pieces; 2, humidifying and preheating the pieces, and performing fiber separation through a defibrator; 3, applying urea resin, an ammonium chloride curing agent and a paraffin waterproofing agent after fiber mixing, and uniformly mixing; 4, drying the fiber mixture; 5, screening the dried fibers, and manufacturing the fiber rough board through hot pressing of a hot press; 6, cooling the rough board, cutting edges and sanding, so as to obtain the finished product medium-density fiberboard. The medium-density fiberboard manufactured through the method provided by the invention is smooth in surface, uniform in fiber texture, moderate in density, good in dimensional stability, good in machining performance, high in bonding strength, static bending strength and plane tensile strength, good in elastic modulus, strong in bolt holding force, and low in moisture absorption and water absorption performance. According to the method, the branches and trunk of ricinus communis and stalks of miscanthus are used to produce the fiberboard, that branches and trunk of ricinus communis are cut down in successive years for updating is achieved and the forest fire caused by stalks of miscanthus is eliminated, and the method is suitable for areas, where ricinus communis and miscanthus grow.

The invention relates to a plant source pesticide and a preparation method thereof. The pesticide is composed of the following raw materials: selinum japenious seed, officinal magnolia bark, Chinese ephedra, peganum harmala, artemisia scoparia, pokeberry root, mirabilis jalapa, pyrethrin, furocoumarin, ethylicin, rotenone, ricin, sodium alginate and sodium cyclamate. The preparation method comprises the following steps: mixing part of the raw materials and water to form a solution; then performing mixed stirring on a water extraction solution of the part of the raw materials, and adding the remaining raw materials to prepare the plant source pesticide. The prepared plant source pesticide acts on pests, weeds and bacteria, various virus ways and mechanisms are adopted, the pests are not directly killed in general, the growth of a pest population is inhibited, the selectivity is high, and natural enemies of the pests are protected favorably; due to substances which naturally exist, the safety is high, residues are not easily generated after the plant source pesticide is applied to crops, and the phenomenon of biological concentration is not easily caused.

The invention relates to a method for extracting ricinus communis oil by using an aqueous enzymatic method. The method comprises the following steps of: (1) crushing and soaking; (2) carrying out enzyme reaction; and (3) carrying out filtration and centrifugal separation. The invention also relates to a device for extracting the ricinus communis oil by using the aqueous enzymatic method. The method and the device have the advantages that the process is simple and convenient, the equipment is simple, operating conditions are mild, the extracting temperature is low, organic solvents are not required for being utilized to assist extraction, fat and protein resources in ricinus communis seeds can be comprehensively utilized, and the oil yield and fat quality of the ricinus communis seeds are improved.

The invention discloses a breeding maintaining method of ricinus communist female plants. According to the invention, ricinus communist female plants are subjected to tissue culturing, such that ricinus communist female plant breeding maintenance is realized. The tissue culturing process comprises the steps that: (1) axillary buds of ricinus communist female plants are adopted as a material, and is washed by using running water; (2) sterilizing is carried out; (3) induced differentiation culture is carried out; (4) proliferation culture is carried out; and (5) rooting culture is carried out. With the method provided by the invention, a survival rate of female plant axillary bud in-vitro culture is high, a propagation speed is high, and a multiplication coefficient reaches 5.88.

The invention provides a method for planting ricinus communis in a saline and alkaline land in a drought region. According to the method, the pH value of soil is improved with the combination of a microbial fertilizer, earthworms and calcium superphosphate, and fertilizers are provided for ricinus communis to grow; due to adoption of recycled building materials, the moisture utilization rate can be increased; with the combination of ricinus communis planting and saline and alkaline land improvement, the yield of the ricinus communis can be increased year by year.

The invention discloses an anti-ricin immunoglobulin F(ab')2 and a preparation method thereof. The method for preparing the anti-ricin immunoglobulin F(ab')2 comprises the following steps that an animal is immunized by an immunogen, a ricin IgG antibody is prepared, and an Fc segment is removed from the ricin IgG antibody to obtain the anti-ricin immunoglobulin F(ab')2; antigens of the immunogen are a low-toxic or attenuated ricin mutant or recombinant protein thereof, a DNA molecule encoding the low-toxic or attenuated ricin mutant or the recombinant protein thereof or a carrier or an expression cassette or a recombinant bacterium or a recombinant virus expressing the DNA molecule and detoxified natural ricin. According to the method, the healthy animal is immunized by the non-toxic or low-toxic ricin antigen, a high-titer antiserum for neutralizing the ricin is generated, and the specific protective effect on the treatment and prevention of the ricin is achieved.

The present invention relates to a method for diagnosing a liver disease rapidly in an early stage. More particularly, the present invention relates to a monoclonal antibody against asialo α1-acid glycoprotein; a method for diagnosing a liver disease which evaluates asialo α1-acid glycoprotein in a test sample by using said monoclonal antibody; and an diagnostic strip for immunochromatography composed of said monoclonal antibody against asialo α1-acid glycoprotein and Ricinus communis agglutinin (RCA). The diagnostic device of the present invention is convenient to measure the concentration of asialo α1-acid glycoprotein and to diagnose a liver disease rapidly.

The invention discloses a low-cost and quality-promoting fertilizer special for artemisia selengensis. The fertilizer comprises the following raw materials in parts by weight: 6-12 parts of monopotassium phosphate, 20-30 parts of triple superphosphate, 20-30 parts of potash magnesium sulphate fertilizers, 25-45 parts of ammonium chloride, 6-10 parts of fir charcoal, 4-8 parts of bone residues, 10-15 parts of mushroom dreg, 25-45 parts of oil residues, 10-20 parts of silkworm excrement, 3-6 parts of ricinus communis dreg, 10-16 parts of rape seed cake, 4-8 parts of modified kaolin, 2-4 parts of illite, 4-12 parts of montmorillonoid, 4-10 parts of white mica, 10-16 parts of vermiculite power, 1.5-2.5 parts of EDTA chelate manganese, 1-2 parts of zinc sulfate, 0.02-0.06 part of bacillus subtilis and 0.03-0.07 part of phytase. The low-cost and quality-promoting fertilizer special for artemisia selengensis has excellent slow release effect for nutrient substances, can release according to the growth and development requirements of artemisia selengensis, can effectively reconstruct the soil granular structure, can obviously improve soil and can promote the growth and development of the over-ground part of the artemisia selengensis.

The invention discloses a traditional Chinese medicine preparation used for treating intestinal obstruction. The traditional Chinese medicine preparation is prepared from following main raw materials, by weight, 10 to 16 parts of pericarpium citri reticulatae viride, 5 to 12 parts of malt, 6 to 12 parts of magnolia obavata, 5 to 15 parts of folium sennae, 10 to 20 parts of ricinus communis, 3 to 8 parts of betel nut, 4 to 8 parts of szechwan Chinaberry fruit, 10 to 20 parts of pericarpium citri reticulatae, 8 to 16 parts of semen raphani, 9 to 13 parts of black soya bean, 5 to 15 parts of hawthorn, 1 to 5 parts of Sedum aizoon, and 4 to 6 parts of cortex cinnamomi. The traditional Chinese medicine preparation is capable of invigorating spleen and warming the middle warmer, activating qi for lowering adverse qi, removing food retention and promoting purgation, purgating heat and bowels, relieving swelling and pain, resolving hard lump, removing blood stasis and stimulating the menstrual flow, relieving intestinal adhesion, promoting gastrointestinal motility, improving intestinal blood circulation, reducing capillary permeability, protecting intestinal mucosal barrier and reducing bacterial translocation, and promoting inflammatory edema extinction. It is confirmed by clinical experiments that curative effect is excellent, application is convenient, economical, and safe; patient compliance is high; and the traditional Chinese medicine preparation is worthy of clinical expansion.

The present invention provides a protein having an A chain of a ricin-like toxin, a B chain of a ricin-like toxin and a heterologous linker amino acid sequence, linking the A and B chains. The linker sequence contains a cleavage recognition site for a disease specific protease such as a cancer, inflammatory, fungal, viral or parasitic protease. The invention also relates to a nucleic acid molecule encoding the protein and to expression vectors incorporating the nucleic acid molecule. In addition, the invention relates to a method for producing the recombinant protein in yeast, to nucleic acid molecules for use in yeast and to yeast transformed with such nucleic acid molecules. Also provided is a method of inhibiting or destroying mammalian cancer cells, inflammatory cells, cells infected with a virus, a fugus, or parasite, or parasites utilizing the nucleic acid molecules and proteins of the invention and pharmaceutical compositions for treating human cancer, inflammatory disease, viral infection, fugal infection, or parasitic infection.

The invention provides a compound insecticide for controlling aphids, wherein the compound insecticide comprises, by mass, 0.05-99% of a bulk drug and 1-99.95% of a conventional auxiliary material, and the bulk drug comprises, by weight, 5-55% of Angelica lactone, 2-55% of butylidenephthalide, 0-45% of butylphthalide, 0-25% of Ligustilide, and 0-15% of ricinine. The invention further provides preparation and applications of the compound insecticide. The compound insecticide of the present invention is the lactone compound-containing compound preparation used for controlling aphids and having advantages of high efficiency, no residue, low production cost, economy, practicality, environmental protection and excellent comprehensive performance.

The invention belongs to the field of protein toxin detection, and relates to a method and a kit for detecting ricin based on relative DNA walker initiation index amplification of a gold nanoprobe constructed by freezing. The method mainly comprises the following four processes: performing freezing to construct the gold nanoprobe, competitively combining the ricin and the gold nanoprobe with an aptamer, and carrying out a relative DNA walker and index amplification reaction. The method and the kit can be used for detecting the ricin with relatively high sensitivity, good specificity and excellent stability in practical application.

The invention discloses a special soil directional conditioner for planting castor-oil plants in saline-alkali soil and a method for planting castor-oil plants in saline-alkali soil, and belongs to the technical field of saline-alkali soil improvement and crop planting. The special soil directional conditioner for castor planting is prepared from the following components in percentage by weight: 40-45 percent of ardealite, 10-15 percent of furfural residues, 20-25 percent of humic acid, 10-15 percent of decomposed sheep manure and 5-10 percent of silicate. The soil directional conditioner disclosed by the invention considers the growth characteristics of ricinus communis and the characteristics of saline-alkali soil, the component composition is simple, and all the components interact, sothat the emergence rate of ricinus communis in the saline-alkali soil can be increased, and the emergence time is shortened; the effect of strengthening seedlings can be achieved; when the soil directional conditioner is used for castor-oil plant planting, castor-oil plants grow well, have luxuriant branches and leaves and can better adapt to growth in saline-alkali soil, and the yield of castor-oil plant seeds is remarkably increased.

A cream composition for massage and sport is disclosed herein. The cream composition includes ricinus communis (castor) seeds oil, sesamum indicum (sesame) seeds oil, deionized water, isopropyl myristate, triticum vulgare (wheat) germ oil, butyrospermum parkii (shea) butter, white iodine, cetostearyl alcohol, a fragrance, sodium laureth sulfate (SLES), bentonite, dmdm hydantoin, sodium chloride, benzyl alcohol, and behydroacetic acid. The composition is administered topically to assist with comforting muscular pain, increasing flexibility, and improving overall skin health.

The invention belongs to the field of toxin detection, and relates to a method for rapidly detecting ricin through colloidal gold lateral flow chromatography, colloidal gold lateral flow chromatography test paper and a kit. The method comprises the following steps that: (1) an H1-AuNPs-FAM antibody, an FAM antibody-AuNPs and a to-be-detected sample containing ricin areincubated; the sequence of H1 is as shown in SEQ ID NO: 1, the particle size of the AuNPs is 2 to 10 nm; (2) a mixed solution obtained by incubation in the step (1) is dropwise added onto lateral flow chromatography test paper, the lateral flow chromatography test paper comprises a sample pad, a detection area, an absorption pad and an NC membrane with a plastic back card, the detection area comprises a T line binding area and a C line binding area, the T line binding area is provided with a detection line, and the C line binding area is provided with a control line; and (3) a detection result is interpreted according to the color development values of colloidal gold nanoparticles on the detection line and the control line. The colloidal gold lateral flow chromatography method for detecting ricin is established. The method does not need to prepare a corresponding antibody, is simple to operate, and can meet the requirements of field detection.

The invention relates to a method for extracting castor oil and synchronously leaching ricinine and allergen toxin from castor bean meal employing an ethanol solution. The castor oil extraction efficiency and the effects for removing ricinine and allergen toxin are as follows; the extraction rate of castor oil is greater than or equal to 99.01%; the residual oil ratio of the bean meal is smaller than or equal to 1.58%; the removal rate of the ricinine is greater than or equal to 96.05%; and the removal rate of the allergen toxin is greater than or equal to 96.94%. The method comprises the following steps: firstly, extracting the castor oil with the high-concentration ethanol solution, and leaching the ricinine from the bean meal; and further leaching the ricinine and the allergen toxin from the bean meal with a low-concentration ethanol solution. According to the overall technology, castor oil extraction is combined with leaching of the ricinine and the allergen toxin in the bean meal, so that serialization of the technology is realized; the industrial cost is reduced; ethanol is adopted as an extracting agent, so that clean production is realized; no three wastes are generated; environmental protection is facilitated; comprehensive utilization of castor seeds is realized; the additional value of a castor seed by-product is improved; and the method has good application value.

The present invention provides a breeding method for tetraploid ricinus communis. The method comprises: collecting a ricinus communis germplasm resource, performing morphologic character analysis and chromosome ploidy identification, selecting good diploid varieties, carrying out mutagenesis by using colchicine and oryzalin; performing chromosome ploidy identification on F1 generation seeds, selecting homozygous tetraploids, and performing economic character analysis and identification on the F2 generation, so as to breed good tetraploid ricinus communis.

The invention discloses a foot cream for curing diabetic foot and a preparation method of the foot cream, and belongs to the technical field of treatment of diabetic complications. The foot cream is prepared from the following components in parts by mass: 40-60 parts of pearl powder, 8-16 parts of pseudo-ginseng, 7-12 parts of coptidis rhizoma, 8-15 parts of buthus martensi karsch, 7-13 parts of centipede, 9-14 parts of angelica sinensis, 8-14 parts of raw common monkshood mother roots, 10-15 pars of baical skullcap roots, 8-13 parts of cortex phellodendri, 28-34 parts of gypsum, 13-18 parts of kakuda figwort roots, 27-31 parts of ricinus communis, 13-18 parts of euphorbia kansui roots, 45-60 parts of vaseline, 29-34 parts of pix fabate nigrate, 28-31 parts of eucalyptus oil, 13-16 parts of borneol, 3-6 parts of triglyceride, 27-33 parts of a cortex pseudolaricis extract, 4-8 parts of a licorice root extract, 8-12 parts of zedoary turmeric oil and 40-50 parts of yellow lead. Skin immunologic barriers are improved, inflammation diminishing is achieved and ulcer surfaces are healed, open sores are reduced gradually, and the ulcer surfaces disappear and finally well-healed.

A rodenticide is provided, comprising extracts of: Aesculus hippocastanum, Prunus laurocerasus, Digitalis purpurea, Melilotus officinalis, Ricinus communis, Salix alba, egg albumin, and Penicillium chrysogenum.