50 results about "Ricinus sanguineus" patented technology

The present invention is directed to protocells for specific targeting of hepatocellular and other cancer cells which comprise a nanoporous silica core with a supported lipid bilayer; at least one agent which facilitates cancer cell death (such as a traditional small molecule, a macromolecular cargo (e.g. siRNA or a protein toxin such as ricin toxin A-chain or diphtheria toxin A-chain) and / or a histone-packaged plasmid DNA disposed within the nanoporous silica core (preferably supercoiled in order to more efficiently package the DNA into protocells) which is optionally modified with a nuclear localization sequence to assist in localizing protocells within the nucleus of the cancer cell and the ability to express peptides involved in therapy (apoptosis / cell death) of the cancer cell or as a reporter, a targeting peptide which targets cancer cells in tissue to be treated such that binding of the protocell to the targeted cells is specific and enhanced and a fusogenic peptide that promotes endosomal escape of protocells and encapsulated DNA. Protocells according to the present invention may be used to treat cancer, especially including hepatocellular (liver) cancer using novel binding peptides (c-MET peptides) which selectively bind to hepatocellular tissue or to function in diagnosis of cancer, including cancer treatment and drug discovery.

Compositions and methods for treatment of toxin poisoning are disclosed.

An odor absorbing composition is provided comprising zinc salt of ricinoleic acid, a solubility promoter including sodium iminodisuccinate, water and optionally, other ingredients such as perfumes and antifungal agents or bactericides. The zinc ricinoleate can be completely solubilized in water, yet the solution will exhibit low foaming and friability. In addition, the end product may be in the form of a sprayable liquid, a thick liquid capable of clinging to a vertical surface, a gel or solid tablet, a powder, or any other form. The present invention further relates to a method for forming an odor absorbing composition comprising zinc salt of ricinoleate acid, sodium iminodisuccinate and water.

The present invention provides a protein having chain of a ricin-like toxin, a B chain of a ricin-like toxin and a novel heterologous linker amino acid sequence, linking the A and B chains. The linker sequence contains a cleavage recognition site for a specific protease such as those found in inflammatory cells and cancer cells. The invention also relates to a nucleic acid molecule encoding the protein and to expression vectors incorporating the nucleic acid molecule. Also provided is a method of inhibiting or destroying cells having a specific protease, such as cancer cells or inflammatory cells utilizing the nucleic acid molecules and proteins of the invention and pharmaceutical compositions for treating human inflammation and cancer.

The present invention relates to the generation of stable haploid cell cultures, uses of said cells in forward and reverse genetics, especially the identification of target genes associated with a modified phenotype and in particular identifying genetic targets associated with toxin resistance, especially ricin toxicity resistance, and therapeutic uses of target compounds.

The invention provides a method for producing a medium-density fiberboard through branches and trunk of ricinus communis and stalks of miscanthus. The method comprises the following steps: 1, after the branches and trunk of ricinus communis and stalks of miscanthus are mixed, cutting the mixture into pieces; 2, humidifying and preheating the pieces, and performing fiber separation through a defibrator; 3, applying urea resin, an ammonium chloride curing agent and a paraffin waterproofing agent after fiber mixing, and uniformly mixing; 4, drying the fiber mixture; 5, screening the dried fibers, and manufacturing the fiber rough board through hot pressing of a hot press; 6, cooling the rough board, cutting edges and sanding, so as to obtain the finished product medium-density fiberboard. The medium-density fiberboard manufactured through the method provided by the invention is smooth in surface, uniform in fiber texture, moderate in density, good in dimensional stability, good in machining performance, high in bonding strength, static bending strength and plane tensile strength, good in elastic modulus, strong in bolt holding force, and low in moisture absorption and water absorption performance. According to the method, the branches and trunk of ricinus communis and stalks of miscanthus are used to produce the fiberboard, that branches and trunk of ricinus communis are cut down in successive years for updating is achieved and the forest fire caused by stalks of miscanthus is eliminated, and the method is suitable for areas, where ricinus communis and miscanthus grow.

The invention discloses a breeding maintaining method of ricinus communist female plants. According to the invention, ricinus communist female plants are subjected to tissue culturing, such that ricinus communist female plant breeding maintenance is realized. The tissue culturing process comprises the steps that: (1) axillary buds of ricinus communist female plants are adopted as a material, and is washed by using running water; (2) sterilizing is carried out; (3) induced differentiation culture is carried out; (4) proliferation culture is carried out; and (5) rooting culture is carried out. With the method provided by the invention, a survival rate of female plant axillary bud in-vitro culture is high, a propagation speed is high, and a multiplication coefficient reaches 5.88.

The invention discloses a low-cost and quality-promoting fertilizer special for artemisia selengensis. The fertilizer comprises the following raw materials in parts by weight: 6-12 parts of monopotassium phosphate, 20-30 parts of triple superphosphate, 20-30 parts of potash magnesium sulphate fertilizers, 25-45 parts of ammonium chloride, 6-10 parts of fir charcoal, 4-8 parts of bone residues, 10-15 parts of mushroom dreg, 25-45 parts of oil residues, 10-20 parts of silkworm excrement, 3-6 parts of ricinus communis dreg, 10-16 parts of rape seed cake, 4-8 parts of modified kaolin, 2-4 parts of illite, 4-12 parts of montmorillonoid, 4-10 parts of white mica, 10-16 parts of vermiculite power, 1.5-2.5 parts of EDTA chelate manganese, 1-2 parts of zinc sulfate, 0.02-0.06 part of bacillus subtilis and 0.03-0.07 part of phytase. The low-cost and quality-promoting fertilizer special for artemisia selengensis has excellent slow release effect for nutrient substances, can release according to the growth and development requirements of artemisia selengensis, can effectively reconstruct the soil granular structure, can obviously improve soil and can promote the growth and development of the over-ground part of the artemisia selengensis.

The present invention provides a protein having an A chain of a ricin-like toxin, a B chain of a ricin-like toxin and a heterologous linker amino acid sequence, linking the A and B chains. The linker sequence contains a cleavage recognition site for a disease specific protease such as a cancer, inflammatory, fungal, viral or parasitic protease. The invention also relates to a nucleic acid molecule encoding the protein and to expression vectors incorporating the nucleic acid molecule. In addition, the invention relates to a method for producing the recombinant protein in yeast, to nucleic acid molecules for use in yeast and to yeast transformed with such nucleic acid molecules. Also provided is a method of inhibiting or destroying mammalian cancer cells, inflammatory cells, cells infected with a virus, a fugus, or parasite, or parasites utilizing the nucleic acid molecules and proteins of the invention and pharmaceutical compositions for treating human cancer, inflammatory disease, viral infection, fugal infection, or parasitic infection.

The invention provides a compound insecticide for controlling aphids, wherein the compound insecticide comprises, by mass, 0.05-99% of a bulk drug and 1-99.95% of a conventional auxiliary material, and the bulk drug comprises, by weight, 5-55% of Angelica lactone, 2-55% of butylidenephthalide, 0-45% of butylphthalide, 0-25% of Ligustilide, and 0-15% of ricinine. The invention further provides preparation and applications of the compound insecticide. The compound insecticide of the present invention is the lactone compound-containing compound preparation used for controlling aphids and having advantages of high efficiency, no residue, low production cost, economy, practicality, environmental protection and excellent comprehensive performance.

The invention discloses a special soil directional conditioner for planting castor-oil plants in saline-alkali soil and a method for planting castor-oil plants in saline-alkali soil, and belongs to the technical field of saline-alkali soil improvement and crop planting. The special soil directional conditioner for castor planting is prepared from the following components in percentage by weight: 40-45 percent of ardealite, 10-15 percent of furfural residues, 20-25 percent of humic acid, 10-15 percent of decomposed sheep manure and 5-10 percent of silicate. The soil directional conditioner disclosed by the invention considers the growth characteristics of ricinus communis and the characteristics of saline-alkali soil, the component composition is simple, and all the components interact, sothat the emergence rate of ricinus communis in the saline-alkali soil can be increased, and the emergence time is shortened; the effect of strengthening seedlings can be achieved; when the soil directional conditioner is used for castor-oil plant planting, castor-oil plants grow well, have luxuriant branches and leaves and can better adapt to growth in saline-alkali soil, and the yield of castor-oil plant seeds is remarkably increased.

The invention relates to a method for extracting castor oil and synchronously leaching ricinine and allergen toxin from castor bean meal employing an ethanol solution. The castor oil extraction efficiency and the effects for removing ricinine and allergen toxin are as follows; the extraction rate of castor oil is greater than or equal to 99.01%; the residual oil ratio of the bean meal is smaller than or equal to 1.58%; the removal rate of the ricinine is greater than or equal to 96.05%; and the removal rate of the allergen toxin is greater than or equal to 96.94%. The method comprises the following steps: firstly, extracting the castor oil with the high-concentration ethanol solution, and leaching the ricinine from the bean meal; and further leaching the ricinine and the allergen toxin from the bean meal with a low-concentration ethanol solution. According to the overall technology, castor oil extraction is combined with leaching of the ricinine and the allergen toxin in the bean meal, so that serialization of the technology is realized; the industrial cost is reduced; ethanol is adopted as an extracting agent, so that clean production is realized; no three wastes are generated; environmental protection is facilitated; comprehensive utilization of castor seeds is realized; the additional value of a castor seed by-product is improved; and the method has good application value.

The present invention provides a breeding method for tetraploid ricinus communis. The method comprises: collecting a ricinus communis germplasm resource, performing morphologic character analysis and chromosome ploidy identification, selecting good diploid varieties, carrying out mutagenesis by using colchicine and oryzalin; performing chromosome ploidy identification on F1 generation seeds, selecting homozygous tetraploids, and performing economic character analysis and identification on the F2 generation, so as to breed good tetraploid ricinus communis.

Particular aspects provide six novel Ricinus communis cDNA clones, including cloned sequences of: DGAT (RcDGAT1 and RcDGAT2); RcLPAT; LACS (RcLACS4), and PDAT (RcPDAT1A and RcPDAT1B). Additional aspects provide methods for substantially enhanced accumulation of hydroxy fatty acid (HFA) in transgenic plant tissue (e.g., seeds), comprising expression of particular novel sequences. For example, expression of RcDGAT2 or RcPDAT1 in castor hydroxylase-expressing Arabidopsis lines resulted in substantially enhanced accumulation of hydroxy fatty acid (HFA) (e.g., to over 30%; a 50-70% increase in HFA accumulation) relative to the hydroxylase-only expressing parental lines. Further aspects provide methods to increase at least one of total lipid content, percent seed germination, and seed weight in transgenic plants, comprising expression of RcDGAT2 in castor hydroxylase-expressing plant lines. Yet further aspects provide methods for expressing and accumulating hydroxyl fatty acid in yeast (e.g., TAG biosynthesis from diricinolein), comprising expression of RcDGAT2 RcDGAT2 coding sequences in yeast.

The invention discloses a compound vegetable oil vaccine adjuvant, which is prepared according to the following method: 1), the compound vegetable oil adjuvant is composed of the following components in volume content: 1.50%-3.5% oleic acid, 9.5%-12.5% ​​Span- 85. 2.50%-3.50% polyglycerol ricinoleate, and the balance is vegetable oil; 2), according to the dosage ratio of 2-8mg ginsenoside / 100ml compound vegetable oil adjuvant, add ginsenoside solution to the compound vegetable oil adjuvant, A compound vegetable oil vaccine adjuvant was obtained. The vaccine prepared with the adjuvant of the present invention is easily absorbed by the body after being injected into animals, is not easy to form vaccine residues at the injection site, can prolong the duration of antibodies, and can better protect immunized animals.

The present invention relates to the technical field of antidiabetic drugs, and specifically to a recombinant protein drug for treating type 2 diabetes. The drug can reduce blood glucose and serum lipid levels in type 2 diabetic rats.