Breeding maintaining method of ricinus communist female plant

Abstract

The invention discloses a breeding maintaining method of ricinus communist female plants. According to the invention, ricinus communist female plants are subjected to tissue culturing, such that ricinus communist female plant breeding maintenance is realized. The tissue culturing process comprises the steps that: (1) axillary buds of ricinus communist female plants are adopted as a material, and is washed by using running water; (2) sterilizing is carried out; (3) induced differentiation culture is carried out; (4) proliferation culture is carried out; and (5) rooting culture is carried out. With the method provided by the invention, a survival rate of female plant axillary bud in-vitro culture is high, a propagation speed is high, and a multiplication coefficient reaches 5.88.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for propagating and maintaining castor bean female strains. Background technique [0002] castor ( Ricinus communist L.) is an important industrial oil source crop and one of the top ten oil crops in the world. It is widely used in chemical production such as high-grade lubricating oil, brake oil and protective oil, paint, plasticizer, nylon series, polyurethane, spices, cosmetics, etc. The development of medical anticancer drugs, the development of biological pesticides, the refining of biodiesel and the production of high-quality feed and other fields. The use of castor oil to generate chemical derivatives has reached 175 kinds, with more than 2,000 series of products. At present, the demand for castor oil at home and abroad is on the rise, and there is an obvious gap between the supply and demand of raw materials. The world's annual demand for ca...

AI Technical Summary

Problems solved by technology

At present, the demand for castor oil at home and abroad is on the rise, and there is an obvious gap between the supply and demand of raw materials. The world's annual demand for castor oil is about 700,000 tons, but the supply capacity is only 400,000 tons. The domestic annual demand for seeds is about 400,000 tons. The volume is less than 200,000 tons, and the gap is nearly half

Using environmental high temperature to induce male flowers to maintain female lines, although relatively mature, such as Zimmerman, Ankineedu, etc. have bred '240', 'SKP' series, 'VP-1' temperature-sensitive female lines, but the method of high-temperature induction of male flowers is limited by materials and environmental conditions Limitations, only suitable for temperature-sensitive materials, and have requirements for daily average temperature

The mechanical damage method is time-consuming and has a low success rate, and the specific method has not been reported

Although the high-altitude crimping method has a high reproductive coefficient and the female rate of the female line can be increased to more than 98%, it is labor-intensive, time-consuming, and costly, and is only suitable for perennial female castor.

The method of using plant growth regulators or chemical agents to induce male flowers has been reported, but whether the material used in the research is a female line with 100% female plant (sterile plant) rate is not detailed and conclusive, and the spraying period is generally in flowering At the previous seedling stage, it is difficult to judge whether the male flowers or hermaphrodites that appear are original or induced, and it is difficult to verify the actual effect