Method for rapidly detecting ricin through colloidal gold lateral flow chromatography and colloidal gold lateral flow chromatography kit

A lateral flow chromatography, ricin technology, applied in biological testing, measuring devices, analytical materials, etc., can solve the problems of limited application and promotion, unfavorable on-site use, etc.

Pending Publication Date: 2021-05-18
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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Problems solved by technology

However, these detection methods need to rely on expensive instruments and professional tec...

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  • Method for rapidly detecting ricin through colloidal gold lateral flow chromatography and colloidal gold lateral flow chromatography kit
  • Method for rapidly detecting ricin through colloidal gold lateral flow chromatography and colloidal gold lateral flow chromatography kit
  • Method for rapidly detecting ricin through colloidal gold lateral flow chromatography and colloidal gold lateral flow chromatography kit

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[0034] Preferred embodiments of the present invention will be described in more detail below. Although preferred embodiments of the present invention are described below, it should be understood that the present invention can be embodied in various forms and should not be limited by the embodiments set forth herein.

[0035] LX-200 palm centrifuge, ST70-2 microporous constant temperature oscillator, OSE-DB-02 refrigeration type five-stage programmable metal bath, constant temperature incubator, ricin standard product (Beijing Hanpu Medical and Biological Research Institute), gold Staphylococcus aureus enterotoxin B (SEB) (self-made), fumonisin standard (Shandong Landu Biotechnology Co., Ltd.), bovine serum albumin (BSA) and ovalbumin (OVA) (Sigma-Aldrich), acacia Toxin lectin (Institute of Toxic Drugs, Academy of Military Medical Sciences, Beijing), colloidal gold lateral flow chromatography test strips ( HybriDetect). All nucleotides (ODN) were purchased from Shanghai Sang...

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Abstract

The invention belongs to the field of toxin detection, and relates to a method for rapidly detecting ricin through colloidal gold lateral flow chromatography, colloidal gold lateral flow chromatography test paper and a kit. The method comprises the following steps that: (1) an H1-AuNPs-FAM antibody, an FAM antibody-AuNPs and a to-be-detected sample containing ricin areincubated; the sequence of H1 is as shown in SEQ ID NO: 1, the particle size of the AuNPs is 2 to 10 nm; (2) a mixed solution obtained by incubation in the step (1) is dropwise added onto lateral flow chromatography test paper, the lateral flow chromatography test paper comprises a sample pad, a detection area, an absorption pad and an NC membrane with a plastic back card, the detection area comprises a T line binding area and a C line binding area, the T line binding area is provided with a detection line, and the C line binding area is provided with a control line; and (3) a detection result is interpreted according to the color development values of colloidal gold nanoparticles on the detection line and the control line. The colloidal gold lateral flow chromatography method for detecting ricin is established. The method does not need to prepare a corresponding antibody, is simple to operate, and can meet the requirements of field detection.

Description

technical field [0001] The invention belongs to the field of toxin detection, and in particular relates to a method for rapidly detecting ricin by colloidal gold lateral flow chromatography and a colloidal gold lateral flow chromatography kit for rapidly detecting ricin. Background technique [0002] Castor, the Latin scientific name Ricinus communis L., is native to Somalia and Kenya in northeastern Africa, and is widely distributed in tropical regions of the world. The stems, leaves or castor seeds of castor plants contain toxic substances, mainly including ricin, ricinine, and castor reactants. Ricin (RT) can be dissolved in weak acid or water after chemical purification, and its physical and chemical properties are relatively stable. In terms of chemical structure, ricin is a highly toxic plant protein composed of two peptide chains, A and B. Active ricin enters eukaryotic cells through B-chain-mediated endocytosis, followed by A-chain purification of specific adenine ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/558G01N33/543
CPCG01N33/68G01N33/585G01N33/558G01N33/543G01N2333/415
Inventor 彭媛韩殿鹏白家磊宁保安李双秦康任舒悦李森高志贤盖雪娇
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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