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Color-fluorescence dual-function immunochromatography test strip and preparation method thereof

An immunochromatographic test strip, dual-function technology, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of low detection sensitivity, inability to quantify, unable to provide accurate concentration data, etc., and achieve high detection sensitivity and high detection accuracy. Effect

Active Publication Date: 2016-03-23
南京微测生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the field of immunochromatography detection, there are currently two mainstream detection methods: one is qualitative detection immunochromatography test strips through naked eye interpretation, including colloidal gold-labeled test strips, colored latex-labeled test strips, etc., its advantages It can make a qualitative judgment on the content of the analyte in the sample without configuring any detection equipment. The disadvantage is that it cannot be quantified, cannot provide accurate concentration data, and its detection sensitivity is low. Generally, it can only detect concentrations in ng / The analyte above the ml level; the other is the fluorescent immunochromatographic test strip that is interpreted by the instrument, which has the advantage of providing accurate concentration data through the instrument’s interpretation of the data, and the detection sensitivity is higher than that of colloidal gold or colored latex. The test strip is 2-3 orders of magnitude higher, but its disadvantage is that it is completely impossible to obtain the concentration of the analyte without a reading instrument

Method used

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  • Color-fluorescence dual-function immunochromatography test strip and preparation method thereof
  • Color-fluorescence dual-function immunochromatography test strip and preparation method thereof
  • Color-fluorescence dual-function immunochromatography test strip and preparation method thereof

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preparation example Construction

[0043] A preparation method of a color-fluorescence dual-function immunochromatographic test strip comprises the following steps:

[0044] (I) Preparation of sample pad 2

[0045] Soak glass fiber, polyester film, sponge or filter paper in PBS buffer solution containing EDTA, tween-20, PVP and BSA, then dry in an oven, seal and dry for future use;

[0046] (II) Preparation of marker-binding pad 3

[0047] Soak the glass fiber or polyester film with PBS buffer containing trehalose, bovine serum albumin, tween-20 and PVP, then dry it in an oven, and then spray the color-fluorescent dual-functional microsphere marker probe on the Treat the dried glass fiber or polyester film, place it in an oven for drying again, and store it in a sealed and dry place for later use.

[0048] The specific preparation method of the above-mentioned color-fluorescent dual-functional microsphere marker probe is as follows:

[0049] (a) Preparation of microspheres: Dissolve one of polystyrene, polym...

Embodiment 1

[0061] Aflatoxin M1 blue-time-resolved fluorescent dual-function immunochromatographic test strip

[0062] 1. Preparation method of test strips

[0063] (a) Preparation of microspheres

[0064] Dissolve 8 mmol of styrene monomer and 0.8 mmol of acrylic acid monomer in 10 ml of deionized water containing 0.3 mmol of sodium dodecylsulfonate, add the mixture into a round bottom flask, and stir evenly with a magnetic stirring bar. Then use high-purity nitrogen to remove the air in the round-bottomed flask, seal and heat to 70°C, add 0.3ml of 0.2mmol potassium persulfate, seal the flask with oxygen and stir for 8 hours, then cool down to room temperature. Then filter the reaction solution with a filter paper with a pore size of 8 μm, collect the filtrate and centrifuge and purify it. The centrifugation condition is 50,000 g at 10°C for 15 minutes at a constant temperature, remove the supernatant, redissolve the precipitate with 5 ml of deionized water, wash repeatedly three times,...

Embodiment 2

[0102] Chloramphenicol red-rhodamine fluorescent dual-function immunochromatographic test strips

[0103] 1. Preparation method of test strips

[0104] (a) Synthesis of microspheres

[0105] Dissolve 8 mmol of styrene monomer and 0.8 mmol of acrylic acid monomer in 10 ml of deionized water containing 0.3 mmol of sodium dodecylsulfonate, add the mixed solution into a round bottom flask, and stir evenly with a magnetic stirring bar. Then use high-purity nitrogen to remove the air in the round-bottomed flask, seal and heat to 70°C, add 0.3ml of 0.2mmol potassium persulfate, seal the flask with oxygen and stir for 8 hours, then cool down to room temperature. Then filter the reaction solution with a filter paper with a pore size of 8 μm, collect the filtrate and centrifuge and purify it. The centrifugation condition is 50,000 g at 10°C for 15 minutes at a constant temperature, remove the supernatant, redissolve the precipitate with 5 ml of deionized water, wash repeatedly three ti...

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Abstract

The invention belongs to the field of biological detection, and particularly relates to a color-fluorescence dual-function immunochromatography test strip and a preparation method thereof. The test strip comprises a base plate (1). A sample addition pad (2), a marker combination pad (3), a detection pad (4) and a water absorption pad (5) are sequentially arranged on one face of the base plate (1), wherein the detection pad (4) is provided with a detection line T (41) and a quality control line C (42), and the water absorption pad (5) provides power for a capillary tube siphoning, the adjacent pads are sequentially connected in an overlapped mode, and a color-fluorescence dual-function microsphere marker probe coats the marker combination pad (3). According to the color-fluorescence dual-function immunochromatography test strip and the preparation method thereof, negative and positive attribute preliminary qualitative judgment can be performed on the detection result through naked eyes, the detection result can also be analyzed through an instrument, and accurate detection data are obtained.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a color-fluorescence dual-function immunochromatographic test strip and a preparation method thereof. Background technique [0002] In the field of immunochromatography detection, there are currently two mainstream detection methods: one is qualitative detection immunochromatography test strips through naked eye interpretation, including colloidal gold-labeled test strips, colored latex-labeled test strips, etc., its advantages It can make a qualitative judgment on the content of the analyte in the sample without configuring any detection equipment. The disadvantage is that it cannot be quantified, cannot provide accurate concentration data, and its detection sensitivity is low. Generally, it can only detect concentrations in ng / The analyte above the ml level; the other is the fluorescent immunochromatographic test strip that is interpreted by the instrument, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 肖理文徐秀曹建伟
Owner 南京微测生物科技有限公司
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