A Fluorescent Ratiometric Probe Based on Aggregation-Induced Luminescent Properties and Its Application in the Detection of Hydrogen Peroxide and Glucose

A fluorescence ratio probe and aggregation-induced luminescence technology, which is applied in the intersection of chemistry and materials science, can solve the problems of easy interference and self-quenching of detection results, and achieve the effect of visual semi-quantitative detection and broad application prospects.

Active Publication Date: 2021-08-06
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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Problems solved by technology

[0006] The purpose of the present invention is to provide a fluorescent ratiometric probe based on aggregation-induced luminescent properties and its hydrogen peroxide and glucose detection application in order to solve the problems of self-quenching and interference of detection results in the rapid detection of traditional fluorescent probes

Method used

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  • A Fluorescent Ratiometric Probe Based on Aggregation-Induced Luminescent Properties and Its Application in the Detection of Hydrogen Peroxide and Glucose
  • A Fluorescent Ratiometric Probe Based on Aggregation-Induced Luminescent Properties and Its Application in the Detection of Hydrogen Peroxide and Glucose
  • A Fluorescent Ratiometric Probe Based on Aggregation-Induced Luminescent Properties and Its Application in the Detection of Hydrogen Peroxide and Glucose

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Embodiment 1

[0035] A fluorescent ratiometric probe based on aggregation-induced luminescence properties, which is a composite material of tetraphenylethylene sulfonate and bovine serum albumin-coated gold nanoclusters. According to the different mixing ratios of the two, the color of the composite material appears red , yellow or blue;

[0036] A method for preparing a fluorescent ratio probe based on aggregation-induced luminescent properties, the specific steps are as follows:

[0037] Step 1. Prepare tetraphenylethylene sulfonate solution, weigh a certain amount of 1,2-bis[4-(3-sulfonic acid propoxy)phenyl]-1,2-stilbene sodium salt (BSPOTPE) , dissolved in water with a concentration of 0.1mg / L.

[0038] Step 2: To prepare gold nanoclusters coated with bovine serum albumin, add 5mL of chloroauric acid (10mM) to 5mL of bovine serum albumin solution (50mM), adjust the pH to 10 with sodium hydroxide, and react at 37°C for 12 Hours, bovine serum albumin-coated gold nanoclusters (BSA-Au NC...

Embodiment 2

[0041] Detection of hydrogen peroxide: take 12.5 μL of bovine serum albumin-coated gold nanoclusters (5 mM, as Au in chloroauric acid 3+ Ion concentration meter) and 25 μL of 1,2-bis[4-(3-sulfonic acid propoxy)phenyl]-1,2-stilbene sodium salt (0.1 mg / L) were mixed into a fluorescent ratiometric probe. 50 μL H 2 o 2 The solution (1, 2, 3, 4, 5, 6, 7, 8mM) was mixed with the above-mentioned fluorescent ratio probe, and 100 μL of PBS buffer (10 mM, pH 7) was added, and the volume was adjusted to 500 μL, and reacted at 37 ° C for 30 min, and then used The fluorometer scans the fluorescence emission wavelength of 410-850nm, and records the ratio of fluorescence intensity I and I 0 , I and I 0 The ratio (F 680 / F 490 ), to obtain the fluorescence ratio change value (I 0 -I) / I 0 The linear relationship between the concentration of hydrogen peroxide and C (I 0 -I) / I 0 =0.0322C+0.5347, such as image 3 shown. Using this linear relationship, hydrogen peroxide in unknown sampl...

Embodiment 3

[0043] Glucose detection: Take 12.5 μL bovine serum albumin-coated gold nanoclusters (5mM, as Au in chloroauric acid 3+ Ion concentration meter) and 25 μL of 1,2-bis[4-(3-sulfonic acid propoxy)phenyl]-1,2-stilbene sodium salt (0.1 mg / L) were mixed into a fluorescent ratiometric probe. Mix 100 μL glucose solution (1, 2, 3, 4, 5, 6, 7, 8, 9 mM) with 100 μL glucose oxidase (0.5 mg / L) solution, add 100 μL PBS buffer (10 mM, pH 7), add Add water to the fluorescence ratio probe, add water to make up to 500 μL, mix well, react at 37°C for 30 minutes, then scan the fluorescence emission wavelength of 410-850nm with a fluorometer, and record the fluorescence intensity ratio I and I 0 , I and I 0 The ratio (F 680 / F 490 ), to obtain the fluorescence ratio change value (I 0 -I) / I 0 Linear relationship with glucose concentration C (I 0 -I) / I 0 =0.0705C+0.0407, such as Figure 4 shown. Using this linear relationship, glucose in unknown samples can be quantified. Semi-quantitative...

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Abstract

The invention relates to a fluorescent ratio probe based on aggregation-induced luminescent properties and its hydrogen peroxide and glucose detection application, which belongs to the cross field of chemistry and material science. The probe consists of gold nanoclusters coated with tetraphenylethylene sulfonate and bovine serum albumin. The electrostatic adsorption of the two causes the aggregation-induced luminescence phenomenon of tetraphenylethylene sulfonate, and the mixed solution shows dual emission wavelengths between 410-550nm and 550-850nm respectively. The ratio of the two is different, and the appearance of the mixed fluorescent probe The colors are different. The aggregation-induced luminescence of tetraphenylethylene sulfonate can be used as a reference fluorescent color, and the change value (I 0 -I) / I 0 The linear variation relationship with the analyte concentration C realizes the detection of hydrogen peroxide and glucose, and the fluorescent ratio probe has the advantages of simple, rapid and visual detection of hydrogen peroxide and glucose.

Description

technical field [0001] The invention relates to a fluorescent ratio probe based on aggregation-induced luminescent properties and its application in the detection of hydrogen peroxide and glucose, relates to the preparation of nanomaterials and the detection of hydrogen peroxide and glucose, and belongs to the cross field of chemistry and material science. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) is an important reactive oxygen species, widely used in industrial and household washing, bleaching and medical disinfection, but the high concentration of residual hydrogen peroxide in wastewater will cause serious and irreversible oxidative damage to aquatic organisms. On the other hand, hydrogen peroxide, as an important biological signal molecule, plays an important role in various biological processes. Therefore, hydrogen peroxide detection is crucial in both environmental protection and biomedical research fields. [0003] Glucose is an important substance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/78
CPCG01N21/6428G01N21/78G01N2021/6432
Inventor 武晓丽薛健
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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