The invention discloses a conjunctival sac disinfection solution and a using method and device thereof. The conjunctival sac disinfection solution is composed of povidone iodine, sodium iminodisuccinate, potassium iodate, potassium iodide, sodium hydroxide, sodium chloride, potassium chloride, calcium chloride and purified water. When the conjunctival sac disinfection solution is used, the conjunctival sac disinfection solution is directly loaded into a conjunctival sac washing and disinfection device, the conjunctival sac washing and disinfection device is directly operated onto a sterile table by a patrol nurse, and swashing operation is performed by an assistant or an operator in a sterile environment. The conjunctival sac disinfection solution does not need to be prepared and sucked onsite; the solution is prepared by strictly executing sterile operation in an environment meeting requirements; therefore, a sterile product can be formed and can be directly swashed by the assistantor the operator in the sterile environment, the infection risk caused by repeated suction of a rubber plug is reduced, secondary pollution in the self-preparation process is avoided, meanwhile, one device is used for one person, manpower resources and material waste generated in a conventional disinfectant preparation process are saved, and the sterile operation principle is followed.