Snakegourd fruit tissues culture method

Abstract

The invention discloses a snakegourd fruit tissues culture method, which comprises steps: environmental disinfection, selection and processing of explants, preparation of culture mediums, inoculation of the explants, cultivation of culture rooms, greenhouse and greenhouse seedling cultivation and transplant. The snakegourd fruit tissues culture method greatly reduces growing-seedling period and growing-seedling survival rate by culturing nakegourd fruit tissues. The snakegourd fruit tissues culture method has the beneficial effects that the snakegourd fruit tissues culture method is simple and effective, is excellent in disinfection effect of the explants, and can achieve above 95% sterility rate after the explants are disinfected. The explants sprout and grow in aseptic conditions, overcome the influence of environment, improve survival rate of seedling emergence, and are transplanted to plant in seeding trays and grow in a greenhouse after small seeding grows to a certain length, thereby enabling quality of snakegourd fruits to be excellent and healthy, and greatly improving survival rate of field production of the snakegourd fruits,.

Description

technical field [0001] The invention relates to the field of plant biotechnology and cultivation technology, in particular to a method for cultivating seedlings of the trichosanthes group. Background technique [0002] Gualou, Cucurbitaceae Trichosanthes is a perennial climbing herb, up to 10 meters long. The rhizome is thick, cylindrical, and the outer skin is yellow. Stem much branched, glabrous; leaves alternate, nearly round or heart-shaped, dioecious; male flowers several racemes, rarely solitary, corolla lobes obovate, female flowers solitary, ovary ovate, fruit nearly spherical, Orange-red when ripe, flower and fruit period from July to November. [0003] Gualou has antipyretic and thirst-quenching, diuretic, antitussive and expectorant effects. Seeds contain fatty flower oil; fruits contain triterpenoid saponins, organic acids, resins, sugars, and pigments; roots contain proteins, saponins, and acids. Trichosanthes is rich in nutritional value and rich in nutrien...

AI Technical Summary

Problems solved by technology

At present, there are low efficiency in the planting process of melon basket seedlings, mainly including the following aspects: first, the technology of cultivating seedlings of Trichosanthes trichosanthes group is incomplete and not complete, the phenomenon of dead seedlings and rotten seedlings is serious in the production process, and the production efficiency is low; second, Improper use of hormone concentration in the process of group cultivation of seedlings, use too low causes low reproductive efficiency of Trichosanthes trichosanthes, and excessive use causes Trichosanthes to produce deformed flowers or even no flowering; thirdly, the existing method of group cultivation of seedlings takes root slowly, and prone to root rot