Rapid tissue culture propagation method for miscanthus floridulu somatic embryo of mscanthus plant

Abstract

The invention discloses a rapid tissue culture propagation method for miscanthus floridulu somatic embryo of mscanthus plant, comprising the following steps: A, selecting young ear: selecting the yound ear at Ying flower primordium formation period; B, carrying out primary induction with a primary culture medium: cutting to small sections, sterilizing with alcohol, soaking with mercuric chloride,washing and inoculating on the primary induction culture medium; C, subculturing seedling with a seedling culture medium: subculturing and generating the seedling on a subculture culture medium with the obtained embryonic callus, subculturing, multiplication and shining every day; D, carrying out radication induction with a radication induction culture medium; carrying out the induction radication on the radication induction culture medium by seedling, culturing and shining every day; E, acclimating and transplanting: transplanting the tissue culture seeding to an acclimation nursery garden consisting of vermiculite to acclimate, spraying acclimation culture solution and clean liquid to the seeding at fixed time every day, transplanting to the nursery garden and obtaining the miscanthus floridulu seeding. The method is easy to apply, simple to operate, and large in breed coefficient; and the recovery reproduction rate is high, which is about 100%.

Description

technical field [0001] The present invention relates to five-jointed awn ( Miscanthus floridulu ) technology field of tissue culture and rapid propagation based on embryogenic callus, more specifically relates to a method for rapid propagation of somatic embryos of Miscanthus penicillium through tissue culture, which is suitable for callus induction using young ears of Penicillium as explants Ways to obtain seedlings, as well as value addition, rooting, domestication and transplanting. Background technique [0002] In the 1990s, there were relevant reports on the tissue culture and rapid propagation technology of Miscanthus in China. They used young leaves, hypocotyls, young roots, and young ears as explants for the rapid propagation of Miscanths. , but their callus rate and differentiation rate are different, the reproductive efficiency of the young panicle is the highest, but no embryogenic callus was obtained. In addition, in 2005, there was also a report on the...

AI Technical Summary

Problems solved by technology

[0002] In the 1990s, there were relevant reports on the tissue culture and rapid propagation technology of Miscanthus in China. They used young leaves, hypocotyls, young roots, and young ears as explants for the rapid propagation of Miscanths. , but their callus rate and differentiation rate are different, the reproductive efficiency of the young panicle is the highest, but no embryogenic callus has been obtained.

In addition, in 2005, there was also a report on the tissue culture and rapid propagation technology of Miscanthus plants. The stem cutting technology was used to obtain seedlings, but this is also the organogenesis pathway used, and no embryogenic callus was obtained.

Images