A kind of efficient propagation method of Gerbera detoxification seedlings

Abstract

The invention discloses a high-efficiency multiplication method for virus-free gerbera seedlings, which comprises the following steps: 1) pretreatment of gerbera chrysanthemum virus-free seedlings; 2) induction of adventitious buds; 3) cutting adventitious buds for subculture; 4) Seedling hardening; 5) Hydroponic rooting treatment; 6) Hydroponic strong seedlings; 7) Transplanting. The high-efficiency multiplication method of the present invention solves the problems of low efficiency and easy re-pollution during the multiplication process of the virus-free gerbera seedlings. The problem of variety degeneration caused by chrysanthemum branch propagation and seed propagation is simple, easy to promote in factories, and has high practical value in production.

Description

technical field

[0001] The invention relates to a method for efficiently multiplying virus-free gerbera seedlings, in particular to a method for multiplying a large number of good gerbera-free seedlings in a short period of time, and belongs to the technical field of flower planting. Background technique

[0002] Gerbera ( Gerbera jamesonii Bolus), also known as gerbera and sunflower, is a perennial herb of the Compositae family. It is native to the Devasland region of South Africa, and then introduced to the UK. Now it is widely cultivated all over the world. Gerbera likes warm winters and cool summers. The flowers are large, delicate and upright, elegant and elegant, colorful but not coquettish, as beautiful as clouds, charming and elegant, refreshing and pleasing to the eye, giving people a sense of chic, soft, tranquil and serene. Gerbera is often used to cut flowers and place in vases. If it is paired with green foliage plants such as Kidney Tibetan and Asparagus, it ...

Examples

Embodiment 1

[0049] The high-efficiency multiplication method of the virus-free gerbera seedlings of the present embodiment was carried out in 2014-2015. The pre-treatment, plant regeneration and seedling hardening of the gerbera gerbera virus-free seedlings were carried out in Lianyungang Zhenxing Flower Garden, and the tissue-cultured seedlings were transplanted Planted in the flower experiment base of Dongxin Farm in Lianyungang City, the detailed steps are as follows:

[0050] 1) Gerbera detoxification pre-emergence treatment:

[0051] The virus-free seedlings of the gerbera variety Sunshine Coast were used to wash them repeatedly with tap water, then washed with a brush dipped in detergent, then rinsed with ultra-pure water, soaked in 70% alcohol for 15 seconds, and then rinsed with sterile water for two times. Add a few drops of Tween 20 solution to 0.1% mercuric chloride and soak for 5 minutes, rinse with sterile water for 5 times, blot dry with filter paper, and transfer the petiol...

Embodiment 2

[0068] The high-efficiency propagation method of Gerbera chrysanthemum virus-free seedlings in this embodiment, implementation time, pre-treatment of gerbera chrysanthemum virus-free seedlings, plant regeneration, seedling hardening, and tissue culture seedling transplanting are all the same as in Example 1, and the culture medium (liquid) The formulation, comparison medium (solution) formulation and operation steps are the same as in Example 1. The biggest difference between this example and Example 1 is that the Gerbera chrysanthemum virus-free seedling variety is replaced by Linglong:

[0069] 1) Gerbera detoxification pre-emergence treatment:

[0070] The virus-free seedlings of the gerbera variety Linglong are used to wash them repeatedly with tap water, wash them once with a brush dipped in detergent, then rinse them with ultra-pure water, soak them in 70% alcohol for 15 seconds, and rinse them twice with sterile water , add a few drops of Tween 20 solution to 0.1% mercu...

technical field

[0001] The invention relates to a method for efficiently multiplying virus-free gerbera seedlings, in particular to a method for multiplying a large number of good gerbera-free seedlings in a short period of time, and belongs to the technical field of flower planting. Background technique

[0002] Gerbera ( Gerbera jamesonii Bolus), also known as gerbera and sunflower, is a perennial herb of the Compositae family. It is native to the Devasland region of South Africa, and then introduced to the UK. Now it is widely cultivated all over the world. Gerbera likes warm winters and cool summers. The flowers are large, delicate and upright, elegant and elegant, colorful but not coquettish, as beautiful as clouds, charming and elegant, refreshing and pleasing to the eye, giving people a sense of chic, soft, tranquil and serene. Gerbera is often used to cut flowers and place in vases. If it is paired with green foliage plants such as Kidney Tibetan and Asparagus, it ...

Examples

Embodiment 1

[0049] The high-efficiency multiplication method of the virus-free gerbera seedlings of the present embodiment was carried out in 2014-2015. The pre-treatment, plant regeneration and seedling hardening of the gerbera gerbera virus-free seedlings were carried out in Lianyungang Zhenxing Flower Garden, and the tissue-cultured seedlings were transplanted Planted in the flower experiment base of Dongxin Farm in Lianyungang City, the detailed steps are as follows:

[0050] 1) Gerbera detoxification pre-emergence treatment:

[0051] The virus-free seedlings of the gerbera variety Sunshine Coast were used to wash them repeatedly with tap water, then washed with a brush dipped in detergent, then rinsed with ultra-pure water, soaked in 70% alcohol for 15 seconds, and then rinsed with sterile water for two times. Add a few drops of Tween 20 solution to 0.1% mercuric chloride and soak for 5 minutes, rinse with sterile water for 5 times, blot dry with filter paper, and transfer the petiol...

Embodiment 2

[0068] The high-efficiency propagation method of Gerbera chrysanthemum virus-free seedlings in this embodiment, implementation time, pre-treatment of gerbera chrysanthemum virus-free seedlings, plant regeneration, seedling hardening, and tissue culture seedling transplanting are all the same as in Example 1, and the culture medium (liquid) The formulation, comparison medium (solution) formulation and operation steps are the same as in Example 1. The biggest difference between this example and Example 1 is that the Gerbera chrysanthemum virus-free seedling variety is replaced by Linglong:

[0069] 1) Gerbera detoxification pre-emergence treatment:

[0070] The virus-free seedlings of the gerbera variety Linglong are used to wash them repeatedly with tap water, wash them once with a brush dipped in detergent, then rinse them with ultra-pure water, soak them in 70% alcohol for 15 seconds, and rinse them twice with sterile water , add a few drops of Tween 20 solution to 0.1% mercu...

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