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A disinfection method of bamboo embryo culturing

A disinfection method and embryo culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effect of simple disinfection method and improved survival rate

Inactive Publication Date: 2012-11-28
ANHUI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, as far as the existing reports are concerned, many of the latest reports on bamboo tissue culture have focused on callus induction and plant regeneration. for plant regeneration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] Now take Cizhu as an example, the non-limiting embodiment is described as follows:

[0013] 1. After cleaning the surface of the seeds, select the seeds with bright color, full grains, and no pests and diseases, peel off the seed shell, soak the seeds in 70% alcohol on the ultra-clean workbench for 5 minutes (stir continuously), and rinse with sterile water 5 times , and then soaked in 0.1% mercuric chloride solution for 30 minutes (stirring constantly), rinsed with sterile water 5 times. Blot dry with sterile paper and inoculate on medium.

[0014] 2. After the surface of the seeds is cleaned, select seeds with bright color, full grains, and no pests and diseases, peel off the seed shells, and soak the seeds in 70% alcohol on the ultra-clean workbench for 5 minutes (stir constantly) and rinse them with sterile water 5 times. Soak in 0.2% sodium hypochlorite for 15 minutes (stirring constantly), rinse with sterile water 5 times, then soak in 70% alcohol for 5 minutes (...

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PUM

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Abstract

The present invention discloses a disinfection method of bamboo embryo culturing, which takes healthy seed of bamboo in the same year as an explant, soaks the grain after shelling in medical alcohol for 4-6 min, and then stirs it in 0.1 wt. % mercuric chloride solution for 25-35 min or in 0. 2-0.3 wt. % sodium hypochlorite solution for 10-20min, at last drops it in medical alcohol for 4-6 min. The disinfection method is simple and can effectively remove surface bacilli and endogenetic bacilli, which lay the foundation of establishing sterilized system, promoting successive transfer culture and rooting, and provides technical characteristics for superior bamboo seed development and utilization.

Description

1. Technical field [0001] The invention relates to a method for rapid propagation of biological tissue culture, in particular to a method for rapid propagation of seed embryo culture, specifically a method for disinfection of bamboo seed embryo culture. 2. Background technology [0002] Bamboo is one of the forest resources. There are more than 70 genera and more than 1,200 species of bamboo plants in the world, mainly distributed in tropical and subtropical regions, and a few bamboos are distributed in temperate and frigid regions. Bamboo is an evergreen (a few bamboo species deciduous in the dry season) shallow-rooted plant, which has high requirements on water and heat conditions and is very sensitive. The distribution of water and heat on the earth's surface dominates the geographical distribution of bamboo. Southeast Asia is located in the tropics and subtropics, and is affected by the monsoons of the Pacific Ocean and the Indian Ocean. It has abundant rainfall and sta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 项艳夏登云高健沈周高卞林玲
Owner ANHUI AGRICULTURAL UNIVERSITY
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