African daisy tissue culture seedling growing method

A bottle seedling and seedling hardening technology, which is applied in the field of plant biotechnology and cultivation, can solve the problems of slow rooting, failure to maintain the stability and purity of the original variety, and no flowering, etc., and achieve the effect of high survival rate

Active Publication Date: 2015-03-11
盐城呈祥园艺育苗有限公司
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  • Summary
  • Abstract
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Problems solved by technology

[0003] Gerbera is a cross-pollinated plant, and the offspring produced by natural or artificial pollination produce trait separation and variability, which cannot maintain the stability and purity of the traits of the original variety; , the limited number of ramets and other reasons are increasingly unable to meet the needs of current flower planting and are basically abandoned
Tissue culture and rapid propagation of seedlings has become the main method for the production of gerbera seedlings, but there ar

Method used

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Embodiment 1

[0019] The method for cultivating seedlings of the gerbera group of the present embodiment is characterized in that the method for cultivating seedlings of the gerbera group comprises the following steps:

[0020] (1) Selection and treatment of explants: select the young unopened receptacles of gerbera plants as explants; wash the explants with running water for 20 minutes, add detergent to soak for 20 minutes, and then rinse with running water 30 min; move the cleaned explants to a beaker in an ultra-clean bench, add 75% ethanol to sterilize for 30 s, and then wash 3 times with sterilized water; add explants to 0.1% mercuric chloride solution for 15 min, and then Wash with sterilized water 7 times;

[0021] (2) Induction of adventitious buds: After the gerbera receptacles treated in step (1) were cut off a little bottom tissue, they were inoculated on the No. 1 medium according to the growth direction of the receptacle. The No. 1 medium was: MS basic culture medium, 6-benzyl...

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Abstract

The invention relates to an African daisy tissue culture seedling growing method which comprises the following steps: (1) explant selection and processing; (2) adventitious bud induction; (3) adventitious bud multiplication; (4) adventitious bud rooting; and (5) seedling hardening and acclimatization. The African daisy tissue culture seedling growing method has good sterilization effect, rooting time is short, growth time is fast, and seedling quality is high.

Description

technical field [0001] The invention belongs to the field of plant biotechnology and cultivation technology, and in particular relates to a method for cultivating seedlings of gerbera chrysanthemums. Background technique [0002] Gerbera, also known as Gerbera, is a perennial herbaceous flower of Compositae. Gerbera chrysanthemums not only have beautiful flower shapes and bright and rich colors, but also have relatively low requirements for production facilities due to cold resistance and high annual production output. In recent years, the gerbera market has seen a boom in supply and demand. The volume is increasing year by year. [0003] Gerbera is a cross-pollinated plant, and the offspring produced by natural or artificial pollination produce trait separation and variability, which cannot maintain the stability and purity of the traits of the original variety; Reasons such as the limited number of ramets are more and more unable to meet the needs of current flower plant...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 裴宝磊陈玉宏陈祥
Owner 盐城呈祥园艺育苗有限公司
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