A kind of decellularized protein scaffold disinfection method

A protein scaffold and disinfection method technology, applied in the field of acellular protein scaffold disinfection, can solve the problems of protein dissolution and denaturation cannot be guaranteed, and achieve the effects of simple and easy operation, good application prospect and low cost of the disinfection method

Active Publication Date: 2020-07-28
SHANGHAI TONGJI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results show that the disinfection effect of PAA is the best, but this disinfection method can only guarantee a good disinfection effect, and cannot guarantee the tissue swelling, protein dissolution, denaturation and other problems

Method used

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  • A kind of decellularized protein scaffold disinfection method
  • A kind of decellularized protein scaffold disinfection method
  • A kind of decellularized protein scaffold disinfection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of Decellularized Protein Scaffold Disinfectant

[0038] 1 Experimental raw materials

[0039] 1000mL deionized water, 58g sodium chloride crystal powder, volume 2.8mL, mass percent concentration 35% peracetic acid solution, 5.6mL 10% sodium hydroxide solution.

[0040] 2 preparation method

[0041]First dilute 2 .8mL of 35% peracetic acid solution with 1000mL of deionized water, then neutralize the diluted peracetic acid solution with 5.6mL of 10% sodium hydroxide solution until the pH is 7.0 -7.5, finally add sodium chloride crystal powder with a concentration of 1.5mol / L and a mass of 58g, and mix well to obtain a disinfection solution.

[0042] The operation of configuring the solution needs to be completed in a laminar flow ultra-clean bench.

[0043] When titrating the peracetic acid solution for the first time, titrate while stirring with the method of electromagnetic stirring, put the pH detection probe in the solution simultaneously, wr...

Embodiment 2

[0044] Example 2 Effect Evaluation of the Disinfection Method of Acellular Protein Scaffold

[0045] 1 Experimental raw materials

[0046] Pig's trachea, human A549 cells, 1000mL deionized water, 58g sodium chloride crystal powder, 2.8mL volume, 35% mass percent peracetic acid solution, 5.6mL 10% sodium hydroxide solution, 0 . 1% SDS solution, 0.1% TritonX solution, PBS solution.

[0047] 2 Experimental methods

[0048] 2.1 Decellularization of porcine trachea

[0049] First cut the pig’s trachea into 3*2cm segments, and perform decellularization in the conical tube. The decellularization protocol is as follows:

[0050] (1) Shake continuously for 30 hours with 0.1% SDS solution, and replace the SDS solution every 6 hours;

[0051] (2) Replace the deionized water and continue shaking for 12 hours, and replace the deionized water every 4 hours;

[0052] (3) Replace the 0.1% TritonX solution and continue to shake for 12 hours, and replace the TritonX solution every 4 hours;...

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Abstract

The invention relates to a new method for disinfecting an accellular protein scaffold. The method comprises the steps of: disinfecting a protein scaffold with a low-concentration peracetic acid neutralized with 10% sodium hydroxide in combination with a hypertonic ion solution, thereby achieving the purpose of killing bacteria and spores in the collagen scaffold without denaturing the protein scaffold and also facilitating the cell growth during late recellularization. The method has the advantages that the new disinfection method is simple and easy to operate, has high disinfection efficiency, can reduce the influence on the physical properties of the accellular protein scaffold and reduce the edema caused by long-time soaking of the protein scaffold, and is beneficial to the late recellularization process.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for disinfecting an acellular protein scaffold. Background technique [0002] Tissue engineering is a research field that explores the relationship between the structure and function of mammalian tissues under normal or pathological conditions through engineering methods and life science principles. The ultimate goal is to develop alternative functional biomaterials to repair, maintain and even improve organizational function. Decellularized protein scaffold is currently a hot spot in the field of tissue engineering and organ regeneration. It has the prospect of reconstructing functional organs and transplanting and repairing the human body. Decellularization of whole organs is the main method to produce protein scaffolds, but the decellularization procedure can only remove the The cell components cannot completely remove the spores in the organ, which is unf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/18A61L101/32A61L101/02
CPCA61L2/0088A61L2202/21
Inventor 林锐施宝民
Owner SHANGHAI TONGJI HOSPITAL
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