A kind of decellularized protein scaffold disinfection method
A protein scaffold and disinfection method technology, applied in the field of acellular protein scaffold disinfection, can solve the problems of protein dissolution and denaturation cannot be guaranteed, and achieve the effects of simple and easy operation, good application prospect and low cost of the disinfection method
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Embodiment 1
[0037] Example 1 Preparation of Decellularized Protein Scaffold Disinfectant
[0038] 1 Experimental raw materials
[0039] 1000mL deionized water, 58g sodium chloride crystal powder, volume 2.8mL, mass percent concentration 35% peracetic acid solution, 5.6mL 10% sodium hydroxide solution.
[0040] 2 preparation method
[0041]First dilute 2 .8mL of 35% peracetic acid solution with 1000mL of deionized water, then neutralize the diluted peracetic acid solution with 5.6mL of 10% sodium hydroxide solution until the pH is 7.0 -7.5, finally add sodium chloride crystal powder with a concentration of 1.5mol / L and a mass of 58g, and mix well to obtain a disinfection solution.
[0042] The operation of configuring the solution needs to be completed in a laminar flow ultra-clean bench.
[0043] When titrating the peracetic acid solution for the first time, titrate while stirring with the method of electromagnetic stirring, put the pH detection probe in the solution simultaneously, wr...
Embodiment 2
[0044] Example 2 Effect Evaluation of the Disinfection Method of Acellular Protein Scaffold
[0045] 1 Experimental raw materials
[0046] Pig's trachea, human A549 cells, 1000mL deionized water, 58g sodium chloride crystal powder, 2.8mL volume, 35% mass percent peracetic acid solution, 5.6mL 10% sodium hydroxide solution, 0 . 1% SDS solution, 0.1% TritonX solution, PBS solution.
[0047] 2 Experimental methods
[0048] 2.1 Decellularization of porcine trachea
[0049] First cut the pig’s trachea into 3*2cm segments, and perform decellularization in the conical tube. The decellularization protocol is as follows:
[0050] (1) Shake continuously for 30 hours with 0.1% SDS solution, and replace the SDS solution every 6 hours;
[0051] (2) Replace the deionized water and continue shaking for 12 hours, and replace the deionized water every 4 hours;
[0052] (3) Replace the 0.1% TritonX solution and continue to shake for 12 hours, and replace the TritonX solution every 4 hours;...
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