A kind of tissue culture large-scale propagation method of cherry seedlings

A technology of tissue culture and cherry, which is applied in the field of plant tissue culture, can solve the problems of restricting large-scale propagation and cultivation of cherry, easily polluted culture, low transplant survival rate, etc., and achieves reduced seedling propagation cost, large reproduction coefficient, The effect of high survival rate of transplanting

CN103299909BInactive Publication Date: 2014-10-01上海闵行区苗圃有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2014-10-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for breeding cherry seedlings in large scales through tissue culturing. The method comprises the following steps of: (1) pretreatment of to-be inoculated materials; (2) disinfection of explants; (3) establishment of sterile systems; (4) multiplication culturing of buds; (5) strong seedling and rooting culturing; and (6) transplantation of test-tube seedlings. The method for rapidly breeding cherry seedlings through tissue culturing has the advantages of a low contamination rate of explants, a high breeding coefficient, good seedling quality and a high transplanting survival rate; according to the method, the defects of a low breeding coefficient, a long period and season limitation of the conventional technology are overcome.
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Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a large-scale propagation method for tissue culture of cherry seedlings. Background technique

[0002] There are more than 120 species of plants in the genus Cerasus, most of which are distributed in Europe and Asia. The most cultivated species in the world are Chinese cherry (Prunus pseudoerasus Lindl.), European sweet cherry (Prunus avium L.), European sour cherry (Prunus cerasus L.) and hair cherry (Prunus tomentosa Thub.). Cherry has excellent characteristics such as tree dwarfing, early fruiting, high yield, strong disease resistance, and wide adaptability. Its fruit is a high-grade fruit with rich nutrition, sweet and sour taste, and high economic value. The traditional propagation method of cherry is root tiller, layering or cutting propagation, which is slow and the growth of seedlings is inconsistent, which cannot meet the needs of large-scale c...

Examples

Embodiment 1

[0036] 1) Pretreatment of inoculated materials: In mid-to-late November, select cherry branches that grow robustly and are free from diseases and insect pests, remove the remaining leaves after natural deciduous leaves, cover the top of the branches with transparent sulfuric acid paper for about 25 cm, and fix the base with paper clips.

[0037]2) Preparation and disinfection of explants: In June of the following year, select freshly pumped semi-lignified shoots, cut off the leaves, wash with detergent, rinse with running water for 3 hours, and use Soak in 75% ethanol solution for 1 min, then soak in 10% sodium hypochlorite solution for 12 min, and rinse with sterile water 4 times.

[0038] 3) Establishment of the sterile line: blot the water on the surface of the explants with sterile absorbent paper, cut the twigs into stem segments of about 1.5 cm, each stem segment has 1 to 2 stem nodes, and cut off the incision For tissues necrotic due to disinfection, the explants were c...