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A selenium-enriched cordycepin and high cordycepin technology applied in the biological field to achieve low cost, high content of cordycepin and high output
Active Publication Date: 2015-03-11
CHONGQING ZONGYI PHARMA
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Problems solved by technology
At present, there is no artificial cultivation method of Cordyceps that ca
Method used
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Embodiment 1
[0021] The cultivation method of embodiment 1 high cordycepin selenium-enriched Cordyceps sinensis
[0022] The method for cultivating high cordycepin selenium-enriched Cordyceps sinensis, according to the following steps:
[0023] 1) Primary solid culture of Cordyceps fungus:
[0024] ①Preparation of slant solid medium: the proportion of medium components is: 120g of potatoes, 12g of glucose, 5g of beef extract, KH 2 PO 4 0.5g, MgSO 4 0.25g, methionine-selenium 0.01g, agar 10g; boil the potatoes first, mash them, add the rest of the above ingredients, heat and boil to melt the agar completely, add distilled water to 500ml, stir evenly, take 40ml test tubes, and fill each tube with culture medium 7ml, make a slope, sterilize with high pressure steam, cool to room temperature and prepare for inoculation;
[0025] ②Inoculation and cultivation: use the slant solid medium prepared in step 1 to inoculate the mycelium 3mm of Cordyceps sinensis under sterile conditions 2 , cul...
Embodiment 2
[0033] Embodiment 2 The cultivation method of high cordycepin-enriched selenium Cordyceps militaris
[0034] The method for cultivating high cordycepin-enriched selenium Cordyceps militaris, according to the following steps:
[0035] 1) Primary solid culture of Cordyceps fungus:
[0036] ①Preparation of slant solid medium: The proportion of medium components is: 110g of potatoes, 11g of glucose, 4.5g of beef extract, KH 2 PO 4 0.4g, MgSO 4 0.2g, methionine-selenium 0.009g, agar 9g; boil the potatoes first, mash them, add the rest of the above ingredients, heat and boil to melt the agar completely, add distilled water to 500ml, stir well, take 40ml test tubes, and fill each tube with culture medium 8ml, make a slope, sterilize with high pressure steam, cool to room temperature and prepare for inoculation;
[0037] ② Inoculation and cultivation: Inoculate 3 mm of mycelium of Cordyceps militaris under aseptic conditions with the slant solid medium prepared in step 1 2 , cu...
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Abstract
The invention discloses a high-cordycepin-content rich-selenium cordyceps sinensis cultivation method. The high-cordycepin-content rich-selenium cordyceps sinensis cultivation method comprises the following steps of 1 cordyceps taishanensis primary solid cultivation, 2 cordyceps taishanensis secondary plant-expanding liquid cultivation and 3 cordyceps sinensis solid productive cultivation. The high-cordycepin-content rich-selenium cordyceps sinensis cultivation method is suitable for artificial cultivation of cordyceps sinensis and cordyceps militaris, obtained cordyceps sinensis is high in cordycepin content and selenium content, the cordycepin content can be 100-200 times of the cordycepin content of wild cordyceps sinensis, the selenium content can reach the selenium content level of the wild cordyceps sinensis, and the effective constituents and effect of the artificially-cultivated cordyceps sinensis are greatly improved. In addition, the high-cordycepin-content rich-selenium cordyceps sinensis cultivation method is high in sundry fungus resisting capacity and yield and has good economic benefit, and the artificial cultivation time can be shortened.
Description
technical field [0001] The invention belongs to the field of biology, and relates to a method for cultivating high-cordycepin-enriched selenium cordyceps. Background technique [0002] There are two types of Cordyceps sinensis and Cordyceps militaris, both belong to the same family but different species. Cordyceps sinensis is a compound of the subpodium and the larvae of the ergot family Cordyceps parasitizing on the larvae of the bat moth. Cordyceps militaris is the abbreviation of Cordyceps militaris, also called Cordyceps militaris or Cordyceps militaris, chrysalis chrysalis, the common name is not old grass, is a medicinal fungus combined with insects and bacteria, modern rare Chinese herbal medicine, Cordyceps militaris belongs to the fungus phylum, Ascomycetes, meat seat Fungi, Ergotaceae, Cordyceps, mainly grow in the northern part of my country. There are two types of Cordyceps, wild and artificial. The active ingredients that play a role in the human body are cord...
Claims
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