Preparation method of selenium-rich cordyceps sinensis mycelia

A technology of selenium-enriched Cordyceps sinensis and selenium-enriched Cordyceps, applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems of unfavorable industrial production and high fermentation cost, and achieve easy industrialization, strong biological activity, and low production cost low effect

Inactive Publication Date: 2013-05-01
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to obtain selenium-enriched Cordyceps mycelium with high nutritional and medicinal value, and overcome the disadvantages of high fermentation cost, which is not conducive to industrial production, the present inve

Method used

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  • Preparation method of selenium-rich cordyceps sinensis mycelia
  • Preparation method of selenium-rich cordyceps sinensis mycelia
  • Preparation method of selenium-rich cordyceps sinensis mycelia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The starting strain: the strain Cordyceps sinensis No. 1 was purchased from Zhengda Fungi Technology Development Center, Liangshan County, Shandong Province.

[0023] Seed culture: take a piece of about 20 mm from the mother seed on the prepared slant medium with an inoculation spatula 2 Insert the lawn of different sizes into the seed medium (sucrose 50g / L, peptone 10g / L, yeast extract 10g / L, solvent is water), and put the sample volume in a 100mL / 250mL Erlenmeyer flask at 25°C and 160r / min After fermenting on a constant temperature shaker for 3 days, tiny bacteria balls were formed in the seed liquid.

[0024] Optimize the concentration of brewer's grains in the fermentation medium, choose 10g / L, 20g / L, 30g / L, 40g / L and 50g / L respectively, add 25mg / L Na 2 SeO 3 , the pH value was adjusted to 6, the inoculation amount of the seed liquid was 10%, and the temperature was 22°C, and the rotation speed of the shaker flask was 160r / min, and cultured for 6 days. The fermen...

Embodiment 2

[0027] Taking Cordyceps sinensis as the starting strain, the concentration of brewer's grains is 30 g / L, and the Na 2 SeO 3 , and other conditions were the same as in Example 1 to obtain selenium-enriched Cordyceps mycelium, the results of the amount of mycelium obtained and the amount of organic selenium in the mycelium are shown in the following table.

[0028]

Embodiment 3

[0030] With Cordyceps sinensis as the starting strain, 25 mg / L Na 2 SeO 3 , the pH value was adjusted to 4, 5, 6, 7 and 8, and other conditions were the same as in Example 2 to obtain selenium-enriched Cordyceps mycelium.

[0031]

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Abstract

The invention provides a preparation method of selenium-rich cordyceps sinensis mycelia. The method comprises the following steps of: inoculating cordyceps sinensis stains in a liquid fermentation culture medium with brewer's grains as a matrix; and culturing for 4-8 days under the conditions of 20-30 DEG C and 120-200 r/min to obtain the selenium-rich cordyceps sinensis mycelia in fermentation liquor, wherein the liquid fermentation culture medium is prepared from the following components: 10-50 g/L of brewer's grains, 10-30 mg/L of Na2SeO3 and water serving as a solvent with pH (Potential of Hydrogen) of 4-8. The obtained fermentation liquor can be further separated by the conventional method to obtain the selenium-rich cordyceps sinensis mycelia. Compared with the prior art, the method has the advantages of mild reaction conditions, low production cost, less equipment investment and easiness in realization of industrialization, and the obtained mycelia have stronger biological activity, so that a breakthrough is expected to be made in prevention and treatment of animal diseases.

Description

(1) Technical field [0001] The invention relates to a preparation method of selenium-enriched Cordyceps sinensis mycelium. (2) Background technology [0002] Cordyceps (Cordyceps) is a rare and valuable medicinal fungus endemic to my country. Cordyceps contains a variety of physiologically active substances. Traditional Chinese medicine literature believes that Cordyceps sinensis is sweet and warm in nature, and has the effects of secreting essence and nourishing qi, protecting lungs and kidneys, and stopping bleeding and reducing phlegm. Modern medical clinical research shows that Cordyceps sinensis can treat symptoms such as fatigue, cough, phlegm and blood, night sweats, impotence, spermatorrhea, jaundice, tuberculosis, senile debility, chronic cough and asthma, etc., and has the effect of strengthening and astringent. Cordycepin contained in Cordyceps sinensis can significantly enhance the ability of red blood cells to adhere to tumor cells, inhibit tumor growth and met...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00A23K1/00
Inventor 杨胜利
Owner ZHEJIANG UNIV OF TECH
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