Method for culturing selenium-enriched Cordyceps militaris

A technology of Cordyceps militaris and a cultivation method, which are applied in the directions of botanical equipment and methods, fertilizer mixtures, gardening, etc., can solve the problems of enhancing immunity, limiting the amount of inorganic selenium added, and failing to fully reflect the selenium-enriched Cordyceps militaris.

Active Publication Date: 2011-08-03
湖北圣峰药业有限公司
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Prior art all is to add inorganic selenous acid or sodium selenite in culture medium, and trace selenous acid or sodium selenite can promote the growth of Cordyceps militaris mycelium, but when inorganic selenium reaches in culture medium Mycelia growth is inhibited at 20mg/L, and mycelia cannot grow more than 30mg/L, thus limiting the amo

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment 1

[0027] In the present invention,

[0028] The first step, the cultivation of selenium-enriched strains on the slant of the test tube

[0029] 1. Weigh 200 grams of washed and peeled potatoes and cut them into thin slices, add 1000 ml to boil for 20 minutes, filter with gauze, take the filtrate and put it into a 1000 ml beaker, add 20 grams of agar, 10 grams of protein, 20 grams of glucose, 0.5 grams of potassium dihydrogen phosphate, 15 grams of selenium yeast with a selenium content of 2000 mg / kg, heated in a water bath until the agar is completely dissolved, add hot water to make up 1000 ml, and pack in In the test tube, each test tube is filled with 12 ml, tightly plugged with a cotton plug, and kept sterilized in a portable autoclave at 120°C for 40 minutes. After cooling, the slope becomes solid and ready for use;

[0030] 2. Separation of strains

[0031] Select wild Cordyceps militaris fruiting bodies with strong growth and orange color or artificially cultivated Co...

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PUM

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Abstract

The invention discloses a method for culturing selenium-enriched Cordyceps militaris, which is to increase the selenium content in a culture medium to 50mg/L by using organic selenium yeasts instead of inorganic seleninic acid and sodium selenite, which infect the growth of hyphae, so as to ensure the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg/L. In the invention, the organic selenium yeasts which exert the lest inhibition effect on the growth of the Cordyceps militaris are adopted to increase the selenium content in the culture medium to 50mg/l, the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg/kg, which is 2 times higher than that of the Cordyceps militaris cultured in a culture medium added with inorganic selenium, and the yield of Cordyceps militaris is high.

Description

technical field [0001] The invention belongs to a method for cultivating selenium-rich Cordyceps militaris. Background technique [0002] Selenium is one of the essential trace elements for the human body. It has various pharmacological effects such as anti-cancer, anti-cancer, anti-oxidation, protecting the body from damage by active oxygen free radicals, protecting the heart, resisting liver necrosis, improving immunity, and delaying aging. , Known as the "King of Anticancer". Modern medical research shows that various human diseases such as cancer, cardiovascular and cerebrovascular diseases, diabetes, and high blood pressure are all related to selenium deficiency. [0003] The distribution of selenium in the natural environment is extremely uneven. Two-thirds of my country is in a state of selenium deficiency. In order to improve human health, many scientific research institutions and bioengineering companies convert inorganic selenium into organic selenium through micr...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 徐景辉
Owner 湖北圣峰药业有限公司
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