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Cultivation method of selenium-enriched Cordyceps militaris mother culture

A technology of Cordyceps sinensis and selenium enrichment is applied in the field of breeding method of selenium-enriched cordyceps sinensis, which can solve the problems of limiting the addition amount of inorganic selenium, failing to fully reflect the selenium-enriching cordyceps sinensis, enhancing immunity and the like.

Inactive Publication Date: 2012-05-02
骆新春
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Problems solved by technology

Prior art all is to add inorganic selenous acid or sodium selenite in culture medium, and trace selenous acid or sodium selenite can promote the growth of Cordyceps militaris mycelium, but when inorganic selenium reaches in culture medium Mycelia growth is inhibited at 20mg / L, and mycelia cannot grow more than 30mg / L, thus limiting the amount of inorganic selenium added to the medium, so that the selenium content of the cultivated Cordyceps militaris is generally within 10-20mg / L , failed to fully reflect the role of selenium-enriched Cordyceps militaris in cancer prevention, anti-cancer, immunity enhancement, anti-aging, prevention and treatment of Keshan disease, diabetes, etc.

Method used

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Embodiment Construction

[0018] Embodiment: the first step, the cultivation of selenium-enriched bacterial classification on the slant of test tube: (1), medium composition: 200 grams of potatoes, 10-30 grams of glucose, 10-20 grams of albumen, 15-30 grams of agar, dihydrogen phosphate Potassium 3 grams, 10-50 grams of selenium yeast with a selenium content of 2000 mg / kg. Preparation method: Weigh 200 grams of washed and peeled potato slices, add 1000 ml and boil for 20 minutes, filter, take potato juice and put it into a 1000 ml beaker, add 20 grams of glucose, 20 grams of agar, 10 grams of egg white, diphosphate Potassium hydrogen 05 grams, selenium yeast 15 grams, heated in a water bath until the agar is completely melted, add water to make up 1000 ml, pack in 20-200m.rn test tubes with umbrellas, each test tube is filled with 12 ml, plug it tightly with a cotton plug, and extinguish it under high pressure. Keep the sterilization pot at 120°C for 40 minutes. After the pot is hot, put the test tube ...

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Abstract

A cultivation method of selenium-enriched Cordyceps militaris mother fungi belongs to the technical field of the cultivation of Cordyceps militaris. The cultivation method comprises the following steps: 1) cultivating selenium-enriched strains on a test tube slant; 2) cultivating selenium-enriched Cordyceps militaris liquid strains; and 3) cultivating the selenium-enriched Cordyceps militaris. The cultivation method uses organic selenium yeast which has the lowest inhibitory effect on the growth of Cordyceps militaris, thus the selenium content of the culture medium is up to 50mg / L, the selenium content of the cultivated Cordyceps militaris is up to 35-50mg / kg which is triple of the selenium content of Cordyceps militaris with additional inorganic selenium, and the yield is increased by 20%.

Description

technical field [0001] The invention belongs to the technical field of Cordyceps sinensis cultivation, in particular to a method for cultivating selenium-rich Cordyceps militaris mother species. Background technique [0002] Selenium is one of the essential trace elements for the human body. It has various pharmacological effects such as anti-cancer, anti-cancer, anti-oxidation, protecting the body from damage by active oxygen free radicals, protecting the heart, resisting liver necrosis, improving immunity, and delaying aging. , Known as the "King of Anticancer". Modern medical research shows that various human diseases such as cancer, cardiovascular and cerebrovascular diseases, diabetes, and high blood pressure are all related to selenium deficiency. The distribution of selenium in the natural environment is extremely uneven. Two-thirds of my country is in a state of selenium deficiency. In order to improve human health, many scientific research institutions and bioengin...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 骆新春
Owner 骆新春
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