The invention provides a compound fermentation biological feed and a preparation method thereof. The method comprises the steps of fermentation of base material components, a fermentation process and the like. The compound fermented biological feed is prepared from the following raw materials in parts by weight: 65 to 72 percent of corn, 5 to 12 percent of soybean meal, 9 to 12 percent of rice bran meal, 7 to 10 percent of bran, 1.0 to 1.5 percent of mountain flour, 0 to 0.2 percent of calcium hydrophosphate, 0.5 to 0.9 percent of lysine, 0 to 0.1 percent of methionine, 0.1 to 0.2 percent of threonine, 0 to 0.1 percent of tryptophan, 0.2 to 0.5 percent of table salt, 0 to 0.05 percent of phytase, 0.01 to 0.03 percent of multi-vitamin and 0.2 to 0.3 percent of multi-mineral. The six fermentation strains, namely lactobacillus rhamnosus, lactobacillus plantarum, bacillus licheniformis, bacillus subtilis, saccharomyces cerevisiae and candida utilis, are adopted. According to the present invention, the high-quality biological fermentation feed is produced by using the conventional complete feed, the nutrition is balanced, the palatability is good, the concentration of ammonia gas and hydrogen sulfide in the manure odor of the fattening pigs can be significantly reduced by more than 60%, and the air environment of the pig house is improved; meanwhile, the feed conversion ratio can be reduced by 14.5%, the production performance is effectively improved, and the feed cost is remarkably reduced.