Method used for artificially cultivating Chinese caterpillar fungus in low altitude region

A technology for artificial cultivation and Cordyceps sinensis, which is applied in the directions of botanical equipment and methods, cultivation, and application to achieve the effects of ensuring generational circulation, broad market application prospects, and improving production efficiency and yield.

Active Publication Date: 2015-11-25
四川藏宝虫草生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high environmental requirements in the cultivation process of Cordyceps sinensis, it is usually cultivated in high-altitude areas. How to cultivate Cordyceps sin...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Strain preparation:

[0027] Select complete and fresh wild Cordyceps, rinse with sterile water overnight, peel off the insect body part on a clean workbench, pick out the inside mycelia for cultivation, and grow fruiting bodies on the medium slope after 90-120 days of cultivation according to the growth condition. The fruiting body that meets the requirements is selected for purification and expansion, and the production and fermentation expansion is carried out after the molecular identification confirms that the strain is correct. The strain is the Mortierella sinensis strain whose strain number is KD2B201.

[0028] (2) Host project:

[0029] 1) The first-generation bat moth pupae collected in the wild were cultured with filter paper to keep moisture. After eclosion into adults, their species was identified through morphological identification. The adults were arranged in eclosion tents, waxed paper was spread on the bottom of the eclosion tents, and branches we...

Embodiment 2

[0039] (1) Strain preparation:

[0040] Same as Example 1.

[0041] (2) Host project:

[0042] 1) The first-generation bat moth pupae collected in the wild were cultured with filter paper to keep moisture. After eclosion into adults, their species was identified through morphological identification. The adults were arranged in eclosion tents, waxed paper was spread on the bottom of the eclosion tents, and branches were placed on the wax papers. For adults to cling to and lay eggs. After laying eggs, the eggs are collected and put into an incubator for cultivation, wherein the humidity of the eggs is kept by conventional filter paper moisturizing methods, the cultivation temperature is 12° C., and the cultivation time is 30 days. Rinse with sterile water after incubation.

[0043] 2) After the eggs hatch, the larvae are raised in a simulated alpine meadow environment. The substrate layer for insect rearing is mainly peat soil, peat soil, sandy soil, and vermiculite, with a w...

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Abstract

The present invention discloses a method used for artificially cultivating Chinese caterpillar fungus in a low altitude region, and relates to various technology characteristics such as host engineering, bacterial strain engineering, infect engineering and germination engineering and the like, the various technologies are effectively combined, steps are implemented accurately, and finally the Chinese caterpillar fungus, components and appearance of which are identical with wild Chinese caterpillar fungus, can be finally cultivated successfully. A sporocarp growth hastening confirmation step is newly added in a bacterial strain preparation step, qualified bacterial strain is ensured to be separated, and production efficiency and yield are improved. In addition, a specific worm cultivation mode is employed, and generation circulation of worm body cultivation is ensured. Comparing the Chinese caterpillar fungus artificially cultivated by the technology with the wild Chinese caterpillar fungus, similarity proportions of protein components exceed 95%, and the method has wide market and application prospects.

Description

technical field [0001] The invention belongs to the technical field of cultivation of medicinal and edible fungi, and relates to a cultivation method of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis [Ophiocordyceps sinensis (Berk.) G.H. Sungetal.] alias Cordyceps, Cordyceps, summer grass winter worm. It is a complex of sub-seats and larval corpses parasitized by Cordyceps sinensis Ophiocordycepssinensis (Berk.) G.H. Sungetal. on the larvae of Hepialus insects of the family Hepialidae. Wild Cordyceps sinensis is distributed in the alpine meadow areas of 3000-5000m in my country's Tibet, Qinghai, Yunnan, Sichuan and Gansu provinces, and has extremely special habitat requirements. Under natural conditions, its life history is extremely complex, and it must go through relatively independent transitions between the conidia stage and the ascospore stage. Its host bat moth takes 5 to 6 years to complete a generation, and more than 85% of the time it lives un...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 冯繻亿吴丽娟
Owner 四川藏宝虫草生物科技有限公司
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