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Preparation method for Ophiocordyceps sinensis strain material and method for infecting Thitarodes larvae with Ophiocordyceps sinensis strain material

A technology of Cordyceps sinensis and Cordyceps sinensis, which is applied to the preparation of Cordyceps sinensis strain materials and the field of infecting the larvae of the hooked bat moth, which can solve the problems of low survival rate, limited yield of strain materials, and long time consumption

Active Publication Date: 2015-03-04
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods have problems such as low infection activity, low survival rate after injury, or too long time-consuming, or limited yield of strain materials, which is one of the key problems restricting the industrialization of original ecological Cordyceps sinensis medicinal materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Collect fresh Cordyceps sinensis from the main producing area of ​​Cordyceps sinensis in Yushu area of ​​Qinghai, adopt conventional broth culture medium to isolate and purify strains, identify as Hirsutella sinensis (Hirsutella sinensis) by molecular biological method or morphological method, and further through common common The broth liquid medium is fermented at 13±5°C for 15 days, and then placed on the solid medium of wheat as the substrate to continue to ferment into Cordyceps sinensis mycelia and conidia or blastospores, and ferment The obtained bacterial mass is evenly crushed with a stirrer, and the suspension obtained by further diluting the mycelia and conidia or blastospores according to the weight of the fermented product to the water volume ratio of 1:1000 is mixed with a sterilized gauze with a pore size of 1.5 mm. After coarse filtration to remove the medium particles, it is used as the source of Cordyceps sinensis.

[0027] 2. In the area of ​​Qingh...

Embodiment 2

[0033] 1. Fresh Cordyceps sinensis was collected from the main producing area of ​​Cordyceps sinensis in Yushu area of ​​Qinghai Province, and the bacterial strain was isolated and purified by conventional Cha's culture medium. It was identified as Hirsutella sinensis by molecular biological methods or morphological methods. The culture medium is fermented at 18°C ​​to form Cordyceps sinensis mycelium and conidia or blastospores of Cordyceps sinensis, and the bacteria balls obtained by fermentation are evenly broken up with a stirrer, and finally the fermented liquid and water volume ratio is 1:100 for further fermentation. The suspension obtained by diluting mycelia and conidia or blastospores is used as the source of Cordyceps sinensis.

[0034] 2. In the alpine meadow area at an altitude of 3,800 meters on the Qinghai-Tibet Plateau, select an area with good terrain drainage, use agricultural sunlight board materials to build a roof and a greenhouse with controllable switches...

Embodiment 3

[0040] 1. Collect fresh Cordyceps sinensis in the Cordyceps sinensis production area of ​​Naqu, Tibet, and use conventional potato culture medium to isolate and purify the bacterial species. It is identified as Hirsutella sinensis by molecular biological methods and morphological methods. Fermentation at ℃ to produce Cordyceps sinensis mycelium and conidia or blastospores of Cordyceps sinensis, the bacteria mass obtained from fermentation is evenly crushed with a stirrer, and the mycelium is further mixed according to the weight of fermented product and water volume ratio of 1:600 and conidia or blastospores to dilute and stir the suspension, and filter the culture medium particles coarsely with gauze with a pore size of 2mm, and use it as the source of Cordyceps sinensis.

[0041] 2. In the alpine shrub meadow area of ​​the Cordyceps sinensis suitable habitat area at an altitude of 4300 meters on the Qinghai-Tibet Plateau, select an area with better terrain drainage and use th...

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PUM

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Abstract

The invention relates to a preparation method for an Ophiocordyceps sinensis strain material and a method for infecting Thitarodes larvae with the Ophiocordyceps sinensis strain material. According to the methods, the principle that the imperfect stage of Ophiocordyceps sinensis--Hirsutella sinensis and plant tissue form a symbiont is employed; a prepared Ophiocordyceps sinensis strain source is inoculated onto the plant tissue; proper conditions are created to promote symbiosis of the Hirsutella sinensis and the plant tissue so as to activate infection activity of the Hirsutella sinensis on the host Thitarodes larvae; the activated Ophiocordyceps sinensis strain Hirsutella sinensis forms the fungus-plant symbiont with the plant tissue; and the fungus-plant symbiont is used as the strain material with high infection activity on the host Thitarodes larvae. The infection rate is up to 85.6 to 98.9% by directly feeding the Thitarodes larvae with the prepared strain material for infection.

Description

technical field [0001] The present invention relates to the preparation of a kind of Cordyceps sinensis fungus material and its method for infecting the larvae of the hooked bat moth Background technique [0002] Cordyceps sinensis Ophiocordyceps sinensis (Berk.) Sung, Sung, Hywel-Jones & Spatafora is a kind of entomocytic ascus fungus, which parasitizes the larvae of Lepidoptera Lepidoptera hook bat moth family Hepialidae hook bat moth genus Thitarodes insects, and its anamorph is Trichosporum sinensis Hirsutella sinensis Liu, Guo, Yu & Zeng. A generation of insects of the genus Thitarodes, the host of Cordyceps sinensis, includes four developmental stages: eggs, larvae, pupae, and adults. The larvae live in underground tunnels and are omnivorous. The young and tender rhizomes of alpine plants such as carrots can also be used as substitute food for common fleshy underground stem plants such as carrots. [0003] Cordyceps sinensis is distributed in the high-altitude areas ...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01H17/00A01K67/033A23K1/18
CPCA01G18/00A01H17/00A01K67/033Y02A40/10
Inventor 钟欣刘昕何健敏
Owner SUN YAT SEN UNIV
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