57 results about "Cellular anabolism" patented technology

The invention provides a group of relevant enzymes for preparing mannitol by performing anabolism on Chinese caterpillar fungus serving as a multifunctional production fungus and hirsutella sinensis based on glucose, a gene for encoding these enzymes and application thereof. The relevant enzymes include (1) hexokinase: manA1-A6 proteins of which the sequences are SEQ ID No.1-6, (2) phosphoglucoisomerase: manB1-B3 proteins of which the sequences are SEQ ID No.7-9, and (3) mannitol-1-P dehydrogenase: manC protein of which the sequence is SEQ ID No.10. In the invention, detailed researches are performed on the metabolic pathway of mannitol synthesized by using Chinese caterpillar fungus serving as a multifunctional production fungus, hirsutella sinensis and glucose on the aspect of principle, cloned DNA (Deoxyribose Nucleic Acid) comprising a nucleotide sequence provided by the invention can be transferred into engineering bacteria with transduction, conversion and conjugal transfer methods, and host mannitol is endowed with high expression by regulating the expression of a biosynthetic gene of the mannitol.

Nutritional compositions are provided containing at least one PPAR agonist, at least one PGC-1alpha agonist, and at least one creatine derivative. Methods of promoting anabolism and improving or enhancing physical performance using the compositions are provided. The compositions and methods can be combined with physical training or exercise.

Vegetarian amino acid sources are combined to create a protein blend that closely matches human muscle tissue in terms of amino acid composition. “Closely matching” means that the amino acid composition of the protein blend is within five percent (5%) of the amino acid composition of human muscle for all of the amino acids of the human muscle. The protein blend can be used as food or food supplement to facilitate and bolster muscle anabolism for healthy humans desiring such result and also to treat and or ameliorate the effects of certain diseases and conditions such as sarcopenia, cancer cachexia, HIV wasting, malnutrition, primary muscle diseases (myopathy) and side effects of corticosteroid therapy.

The invention discloses a corynebacterium glutamicum fermentation culture medium for producing L-isoleucine. The fermentation culture medium comprises a basic culture medium and a growth factor, wherein the growth factor is prepared from choline, betaine and vitamin B6; the content of the choline in the fermentation culture medium is 0.2-1g / L, the content of the betaine in the fermentation culture medium is 0.25-0.5mg / L, and the content of vitamin B6 in the fermentation culture medium is 0.05-0.3mg / L. The fermentation culture medium provides the growth factor for corynebacterium glutamicum, maintains osmotic pressure inside and outside the cells of the corynebacterium glutamicum to be balanced, and promotes the anabolism of amino acid, thus improving the yield and sugar-acid conversion rate of the L-isoleucine.

The invention discloses a fermentation production method based on epothilone B metabolic pathways and belongs to the technical field of fermentation engineering. The method comprises the following steps of: 1) inoculating Sorangium cellulosum into an M26 culture medium, carrying out shake cultivation to obtain a seed solution; 2) inoculating the seed solution into fermentation liquor containing resin, carrying out shake culturing for 72-120 hours at 25-35 DEG C, adding a precursor and small molecular substances into the fermentation liquor containing resin; and 3) filtering the fermentation liquor after the culture is finished, collecting the resin, washing the resin and oscillating and extracting with methyl alcohol to obtain extract liquor containing the epothilone B. According to the invention, the precursor and small molecular substances related to the biosynthetic pathways obtained by screening are added into the fermentation liquor, and the constructive metabolism pathways of the epothilone B are disturbed, so that the excellent bacterial strain gives full play to the ability of anabolism and the fermentation level of the epothilone B is improved greatly, thus the fermentation cost is reduced and the commercial process of the anti-cancer drug is promoted.

The invention relates to a rhodotorula glutinis oil genetic engineering strain and a construction method and an application thereof. The construction method of the genetic engineering strain is mainly as follows: utilizing rDNA (recombinant deoxyribonucleic acid) of rhodotorula glutinis as a target sequence for homologous integration, using strong promoter genes PGK1 of saccharomyces cerevisiae and malate dehydrogenase genes ME of chaetomium cochloides to construct an expression vector to be introduced into rhodotorula glutinis, and enabling ME genes to obtain high-efficient expression in a rhodotorula glutinis body, wherein the content of lipid in a transformant is improved by 2.5 times in comparison with a wild strain. According to the construction method disclosed by the invention, key enzyme genes and a strong promoter for anabolism of the lipid are introduced on the basis that the anabolism of microbial oil is known, so that the lipid metabolism is regulated and controlled, and the yield of oil is improved. The genetic engineering strain can be applied to production of the microbial oil and development of functional oil related products, such as medicaments, health care products and the like.

It has now been discovered that GLP-1 treatment after acute stroke or hemorrhage, preferably intravenous administration, can be an ideal treatment because it provides a means for optimizing insulin secretion, increasing brain anabolism, enhancing insulin effectiveness by suppressing glucagon, and maintaining euglycemia or mild hypoglycemia with no risk of severe hypoglycemia.

The invention relates to a method for producing epsilon-poly-L-lysine. The method is characterized in that a medium containing 1-10g / L of glycine is added at an early or middle stage of culture. By adding glycine in a fermentation process in the invention, glycine which enters a folic acid metabolism approach allows sufficient one-carbon groups to be supplied to a biosynthesis process, the anabolism activity of bacterial strains to be improved, the synthesis of a precursor L-lysine and epsilon-poly-L-lysine to be increased, and the accumulation amount of epsilon-poly-L-lysine to be 20-50% higher than the accumulation amount obtained through original technologies.

Belonging to the field of gene engineering, the invention relates to an AP2 / EREBP transcription factor bNwrI1 gene and an application thereof in adjusting plant seed photosynthesis and oil and fat anabolism. The transcription factor is extracted from brassica napus and is one of the following polypeptides: a polypeptide provided with an amino acid sequence as SEQ ID NO:2 indicates, a polypeptide which is formed with the amino acid sequence replaced, deleted and added by one or more than one amino acid residue and provided with the amino acid sequence and a polypeptide with functions thereof derivating from the polypeptide provided with the amino acid sequence. The transcription factor and coding genes thereof enjoy extensive application value in plant culturing field and can enable the plant to coordinately regulate photosynthesis and fatty acid anabolism, improves seed photosynthesis and oil and fat accumulation capacity, thus improving plant yield and oil content as well as correlated characteristics.

The invention provides a synthetic medium for culturing a phellinus igniarius liquid. The synthetic medium takes glucose as a carbon source and 20 kinds of amino acid as a nitrogen source, and consists of inorganic salt and vitamin. The ratio of carbon to nitrogen is designed as 50 to 1; and the specific concentration g / l is as follows: the glucose: 30 to 80 grams; glutamic acid: 0.1 to 1.5 grams; glutamine: 0.1 to 1.5 grams; aspartic acid: 0.1 to 1.5 grams; asparagines: 0.1 to 1.5 grams; glutamic acid: 0.01 to 0.1 gram; tryptophan: 0.01 to 0.1 gram; valine: 0.01 to 0.1 gram; alanine: 0.01 to 0.1 gram; etc. During the whole culture process of the synthetic medium, phellinus igniarius mycelium is quick in growth; the yield of the phellinus igniarius mycelium can reach 20 grams per liter to the highest degree; simultaneously no agricultural and sideline product is introduced as the carbon source and the nitrogen source; substances such as proteins in the medium, medium compositions and so on are not carried; phellinus igniarius exopolysaccharide produced through fermentation has high purity and stable quality; and the yield of phellinus igniarius polysaccharide can reach 0.198 gram per liter to the highest degree. The synthetic medium has the advantages of clear compositions, stable quality and so on, and can be used for mass culture of the phellinus igniarius liquid and research of the regulation and control rule of polysaccharide anabolism.

The invention relates to a method for applying establishment of gene recombination mobile fermentation monad to alcoholic fermentation. The microorganism is obtained by leading an operon capable of expressing in the mobile fermentation monad, wherein the operon consists of a sequence for coding a promoter and a sequence for coding a structure gene and comprises a sequence coding at least one promoter, and the sequence of the promoter can be identified by the mobile fermentation monad and regulates expression of at least more than one structural gene. The operon comprises a sequence for coding at least one heat shock protein HSP and is used for enhancing the survivability of the mobile fermentation monad in an adverse environment and increasing the temperature of alcoholic fermentation; the operon comprises a sequence for coding at least one lysine anabolism enzyme yfdz so that the mobile fermentation monad can automatically synthesize lysine; the operon comprises a sequence for coding at least one methionine anabolism enzyme metB so that the mobile fermentation monad can automatically synthesize methionine used for lowering the nutrition requirement of the mobile fermentation monad for cultures; and the operon also comprises a sequence for coding a structural gene, which ensures that the mobile fermentation monad can utilize at least one pentaose to generate. The operon enters the mobile fermentation monad in a plasmid transforming way or integrated with the genome of the mobile fermentation monad to obtain a transporton recombinant in a transporton way. The mobile fermentation monad obtained by gene engineering enhances the survivability in an adverse environment, can be fermented to generate the alcohol under higher temperature, obviously lowers the nutrition requirement for the cultures, can utilize the pentaoses to generate the alcohol and still stably retains the characteristics after transfer of culture for 200 times.

The present invention provides an improved method for using the measurement of total urinary 17 KS-S, referred to herein as the urinary Anabolic / Catabolic Index (ACI), as part of a biomarker for biological age.

This invention discloses a kind of genetic engineering bacteria with enhanced tryptophan anabolism, and the recombinant plasmid contained in the genetic engineering bacteria. The recombinant plasmid is prepared by: connecting tryptophan synthetase gene trpBA and phosphoglycerate dehydrogenase gene mutant M3serA gene in series to obtain M3BAT-I, a kind of genetic engineering bacteria with enhanced tryptophan anabolism, expressing induced by IPTG, and analyzing with SDS-PAGE to express tryptophan synthetase beta subunit, phosphoglycerate dehydrogenase M3 mutant, and tryptophan synthetaselpha subunit. After culture of the genetic engineering bacteria with enhanced tryptophan anabolism (tnaA gene removed), the tryptophan content in the supernatant is increased from 2 mg / L to 99 mg / L.

The invention discloses a production and fermentation process of coenzyme Q10. On the premise that the fermentation quality of the coenzyme Q10 is retained, according to the synthesis rate of the main byproduct 5-demethoxylation coenzyme Q10 in the bacteria metabolism process of the coenzyme Q10 as well as the content change thereof, the feedback adjustment is performed on the initial reaction of metabolism, and the speed restriction action is acted on the anabolism, therefore, the feedback adjustment is performed on the accumulation of the product coenzyme Q10 in the cell, the fermenting level of the coenzyme Q10 is greatly promoted through the optimization to the concentration of the main substrate in the fermenting formula and through the optimization control to the stirring rotational speed during the fermenting process and can be improved to above 3000 u / ml, the determination of the optimal time for pot laying improves the utilization ratio of time of the production of the coenzyme Q10 in the cell, the fermentation cost is reduced, and the consumption of the fermenting raw material is greatly reduced.

The present invention provides a set of cordycepin anabolism-related enzymes from bering producing strain cordyceps sinensis hirsutella sinensis-participated ADP ribose starting, genes encoding the enzymes, and application thereof. The related enzymes include proteins shown as SEQ ID No. 1-17, and the coding genes correspond to nucleotide sequences shown as SEQ ID No. 18-34. The invention conducts detailed researches on the principle of metabolic pathways of cordycepin synthesis from bering producing strain cordyceps sinensis hirsutella sinensis ADP ribose. The cloned DNA of the nucleotide sequences provided by the invention can be transferred into engineering bacteria by a transduction, transformation and conjugal transfer method. By adjusting the expression of cordycepin biosynthesis genes, high expression of cordycepin for the host is given.

The invention relates to long-chain-acyl-coenzyme A synthetase for synthesizing metabolic cetyl-coenzyme A with the start that Bailing producing strain cordyceps sinensis hirsutella sinensis is participated in palmatic acid, a gene for coding the enzyme and an application thereof. The amino acid sequence of the long-chain-acyl-coenzyme A synthetase and the sequence represented by SEQ ID No. 1 or SEQ ID No. 3 have more than 90% of homology. According to the invention, the metabolic pathway for synthesizing cetyl-coenzyme A through palmatic acid is researched in principle in a detailed manner as follows: clone DNA of a nucleotide sequence provided by the invention can be transferred into engineering bacteria through transduction, conversion and combined transfer methods; gene expression is biologically synthesized by adjusting the cetyl-coenzyme A; high expressivity of host long-chain-acyl-coenzyme A synthetase is given; an effective way for enlarging the yield of the cetyl-coenzyme A is provided; and the cetyl-coenzyme A has a significant application prospect.

The invention discloses a composite amino acid vitamin injection and the application thereof. Every 1000ml of the composite amino acid vitamin injection comprises 5-40g of L-leucine, 2-30g of L-isoleucine, 2-30g of L-valine, 5-90g of L-alanyl-L-glutamine, 1-5g of L-threonin 1-10g of L-lysine, 1-12g of L-methionine, 1-10g of L-phenylalanine, 0.5-8g of L-tryptophan, 2-30g of L-arginine, 1-10g of L-histidine, 1-100mg of vitamin B6, 0-50mg of vitamin B1 and 0-100mu g of vitamin B12. Aiming at variation of stress, immunity, metabolism and the like of a human body in a perioperative anesthesia period, the composite amino acid vitamin injection achieves the purposes of inhibiting catabolism, promoting anabolism, generating heat and keeping warm, improving body substance energy metabolism and immunity functions and accelerating heal through creative formulae on the basis of enhanced recovery after surgery.

The invention discloses protein with cinnamyl alcohol dehydrogenase activity and a coding gene as well as application thereof. The protein with cinnamyl alcohol dehydrogenase activity provided by the invention is the protein shown as (a) or (b): (a) protein constituted by an amino acid sequence shown as a sequence 2 in a sequence table; and (b) protein derived from (a) by substituting and / or deleting and / or adding one or more amino acid residues for the amino acid sequence shown as the sequence 2 in the sequence table and with the cinnamyl alcohol dehydrogenase activity. By adopting the protein with the cinnamyl alcohol dehydrogenase activity provided by the invention, conversion of coniferyl aldehyde, sinapaldehyde and geranialdehyde into the form of alcohols thereof can be efficiently catalyzed, and conversion of precursor artemisinin aldehyde for artemisinin biosynthesis into artemisinin alcohol can also be catalyzed in an in-vitro enzymatic reaction system, and the later can be applied to adjusting and controlling an artemisinin biosynthesis process from a molecular level; and thus, a foundation is laid for producing a high-yield artemisinin strain.

The present invention makes it possible to efficiently produce an N-methyl transferase with 7-methylxanthine N3 methyl transferase, theobromine N1 methyl transferase, and paraxanthine N3 methyl transferase activities that can be utilized as an industrial, food, or medical enzyme. In addition, the present invention makes it possible to modify the caffeine biosynthesis metabolism of caffeine producing plants, plant tissues, or plant cells, thereby efficiently producing caffeine metabolism based compounds or modifying the production of caffeine in the plants.

The invention provides a novel MYB transcription factor PtrMYB01 in Populus tomentosa Carr, a gene coding the transcription factor, and a cloning method of cDNA, wherein, the transcription factor is polypeptide which is obtained by the separation of Populus tomentosa Carr. By analyzing a highly homologous transcription factor ATMYB60 which belongs the same family with the transcription factor PtrMYB01, the transcription factor PtrMYB01 has possible functions of regulating and controlling plant stress resistance (drought resistance) and regulating anabolism of plant flavonoids. Accordingly, if the recombinant plasmid of the PtrMYB01 gene is transferred into the plant, because the gene is overexpressed in the plant, a drought-resistant cold-resistance plant can be produced, so that the plant yield is increased. The cloning method comprises the following steps: extracting total RNA from Populus tomentosa Carr, and carrying out inverse transcription on the total RNA to synthesize cDNA; designing primers according to the conserved sequence of the transcription factor, and amplifying into a DNA fragment through chain polymerization enzyme reaction; purifying the amplified products, cloning the purified products to a pCXSN vector, transferring a DH5 alfa cell, and culturing overnight in plate. The transferred escherichia coli plasmid is extracted and transferred into Agrobacterium EHA105 for subsequent research.

The invention relates to an adenosine monophosphate (AMP) adenosine deaminase for synthesizing and metabolizing an inosine monophosphate from adenine nucleotide of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application thereof. The AMP adenosine deaminase comprises the proteins shown in SEQ ID No. 1 and SEQ ID No. 2, and the coding gene of the AMP adenosine deaminase corresponds to the nucleotide sequence shown in SEQ ID No. 3 and SEQ ID No.4. According to the invention, the metabolizing way for synthesizing the inosine monophosphate from the adenine nucleotide is researched in detail from the principle, and the cloned DNA containing the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation, and combined transfer; the expression of the gene is biologically synthesized by regulating the inosine monophosphate, the host inosine monophosphate is endowed with high expressivity, an effective way is provided for increasing the yield of the inosine monophosphate, and the invention has a great application prospect.

Isolated novel and purified and characterized phospholipase A2, referred to herein as calcium-independent phospholipase A2zeta (iPLA2zeta) having SEQ. ID. NO: 2 (See FIG. 1), and nucleic acid sequence (SEQ. ID. NO: 4), and calcium-independent phospholipase A2eta (iPLA2eta) having SEQ. ID. NO: 3 (See FIG. 1), and nucleic acid sequences (SEQ. ID. NO: 5). For the first time herein, these novel enzymes have been isolated and characterized and are involved in the catalysis, synthesis and hydrolysis of lipids in a living mammalian cell. Moreover, these enzymes iPLA2zeta and iPLA2eta through the process of transesterification can catalyze the net anabolic synthesis of triglycerides through a variety of metabolic precursors (e.g. monoacylglycerol, diacylglycerol and acyl CoA).

The invention discloses pyruvic acid producing Saccharomyces cerevisiae gene engineering bacteria and a construction method and use thereof and belongs to the field of genetic engineering. In the invention, by knocking out a key regulation gene THI2 in the thiamine synthesis process of Saccharomyces cerevisiae CEN.PK2-1C, the anabolism of the thiamine is interfered to lower the enzymatic activity of a key enzyme for the metabolism of pyruvic acid, so that a carbon metabolic flow to ethanol is reduced and the accumulation of pyruvic acid is promoted. Under a condition of limited addition of thiamine, the Saccharomyces cerevisiae gene engineering bacteria can accumulate pyruvic acid, the method is simple, the yield can reach 6.77+ / -0.14g / L, and thus, a foundation is laid for researching the production of C4 dicarboxylic acid by metabolic engineering modified Saccharomyces cerevisiae.

The invention is concerned with a method and a composition for treating or preventing catabolism or of stimulating anabolism in a mammal undergoing metabolic stress. The method comprises administering to the mammal a composition containing methyl donors selected from the group consisting of L-serine, methionine, choline, phosphatidyl choline, betaine, dimethylglycine, sarcosine, methylated folates, S-adenosyl methionine, thymidine triphosphate, adenosine triphosphate and optionally methyl acceptors selected from the group consisting of L-glycine, ethanolamine, phosphatidyl ethanolamine, folate, ribose, wherein the total molar amount of methyl donors delivered by the method exceeds the total molar amount of methyl acceptors delivered by the method by at least 0.18 mmol per kg of body weight per day.

The invention relates to an inosine monophosphate (IMP) dehydrogenase from 'Bailing' producing strain Cordyceps sinensis Hirsutella sinensis for participating in anabolism on IMP to obtain a xanthylic acid, a gene for encoding the dehydrogenase and application of the dehydrogenase. The IMP dehydrogenase comprises a protein shown by SEQ ID No.1 and a protein shown by SEQ ID No.2, and the coding gene of the protein shown by SEQ ID No.1 and the coding gene of the protein shown by SEQ ID No.2 respectively correspond to a nucleotide sequence shown by SEQ ID No.3 and a nucleotide sequence shown by SEQ ID No.4. According to the invention, the metabolic pathway of the IMP to synthetize the xanthylic acid is traversed from the principle; the cloning deoxyribonucleic acids (DNA) of the nucleotide sequences provided by the invention can be transferred into engineering bacteria through a transduction method, a transformation method and a conjugal transfer method; and through regulating the expression of the biosynthetic gene of the xanthylic acid, the high expressivity of a host xanthylic acid is endowed, and an effective way for increasing the yield of the xanthylic acid is provided. The IMP dehydrogenase has a great application prospect.