Production and fermentation process of coenzyme Q10

A fermentation process and coenzyme technology, which is applied in the direction of fermentation, microorganisms, and methods based on microorganisms, can solve problems such as high cost, inability to meet actual production, and low production level, so as to reduce fermentation cost, improve time utilization, and fermentation level Enhanced effect

Active Publication Date: 2009-09-02
YIDU HEC BIOCHEM PHARMA
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, domestic research on the production of coenzyme Q10 by microbial fermentation and extraction has made some progress, such as the joint use of Guangzhou Institute of Microbiology and Sun Yat-sen University to produce coenzyme Q10 by fermentation of Agrobacterium LQ10, and the enzyme engineering of Shanghai Fangyi Engineering Co., Ltd. Coenzyme Q10 is produced by the method, but the production level is relatively low, the cost is high, and it cannot meet the needs of actual production.

Method used

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  • Production and fermentation process of coenzyme Q10
  • Production and fermentation process of coenzyme Q10
  • Production and fermentation process of coenzyme Q10

Examples

Experimental program
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Effect test

Embodiment 1

[0045] On the slant of the broth culture medium test tube, inoculate Rhodopseudomonas capsulatum on the culture medium under sterile conditions, control the culture temperature at 30°C, and cultivate for 96 hours to grow pale yellow Single colony, the length of the colony is 1-1.5mm, and then the above-mentioned strains are inserted into the sterilized primary seed tank with 7.5L medium under aseptic conditions, and the stirring is controlled under the condition of no need of light The rotation speed is 400rpm, the cultivation temperature is 33°C, the pressure is 0.04Mpa, and the cultivation time is 24 hours, and then transferred to the sterilized secondary seed tank containing 37.5L medium. The temperature is 33°C, the pressure is 0.04Mpa, and the incubation time is 24 hours. The medium formula (by mass ratio) of the primary seed tank and the secondary seed tank is: ammonium sulfate 0.30%, magnesium sulfate 0.25%, potassium dihydrogen phosphate 0.08%, glucose 0.50%, corn stee...

Embodiment 2

[0079] On the slant of the broth culture medium test tube, inoculate Rhodopseudomonas capsulatum on the culture medium under sterile conditions, control the culture temperature at 30°C, and cultivate for 96 hours to grow pale yellow Single colony, the length of the colony is 1-1.5mm, and then the above-mentioned strains are inserted into the sterilized primary seed tank with 1.0 tons of medium under aseptic conditions, and the stirring is controlled under the condition of no need of light The rotation speed is 250rpm, the cultivation temperature is 33°C, the pressure is 0.04Mpa, and the cultivation time is 24 hours; then it is transferred into a sterilized secondary seed tank containing 5.0 tons of medium, and the stirring speed is controlled at 200rpm under the condition of no light, and the cultivation The temperature is 33°C, the pressure is 0.04Mpa, and the incubation time is 24 hours. The medium formula (by mass ratio) of the primary seed tank and the secondary seed tank ...

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Abstract

The invention discloses a production and fermentation process of coenzyme Q10. On the premise that the fermentation quality of the coenzyme Q10 is retained, according to the synthesis rate of the main byproduct 5-demethoxylation coenzyme Q10 in the bacteria metabolism process of the coenzyme Q10 as well as the content change thereof, the feedback adjustment is performed on the initial reaction of metabolism, and the speed restriction action is acted on the anabolism, therefore, the feedback adjustment is performed on the accumulation of the product coenzyme Q10 in the cell, the fermenting level of the coenzyme Q10 is greatly promoted through the optimization to the concentration of the main substrate in the fermenting formula and through the optimization control to the stirring rotational speed during the fermenting process and can be improved to above 3000 u/ml, the determination of the optimal time for pot laying improves the utilization ratio of time of the production of the coenzyme Q10 in the cell, the fermentation cost is reduced, and the consumption of the fermenting raw material is greatly reduced.

Description

technical field [0001] The present invention relates to the improvement of the coenzyme Q10 production process, in particular to a coenzyme Q10 production fermentation process which involves the coenzyme Q10 intermediate metabolism process and its fermentation formula improvement. Background technique [0002] Coenzyme Q10, the chemical name is 2,3-dimethoxy-5-methyl-6-(3-methylbutenyl-2)-benzoquinone, the English name is Ubidecarenone, also known as Coenzyme-10. Coenzyme Q10 is a metabolic activator, which can activate cellular respiration; accelerate the production of adenosine triphosphate (ATP), and play a first-aid role in detoxification; it can change the hypoxic state of cells and tissues, and has a relatively strong effect on the liver, brain, heart and nervous system. Good protection and improvement; it can also enhance non-specific immunity in the body. Coenzyme Q10 is a natural antioxidant produced by the cell itself, which can inhibit the peroxidation of mitocho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/66C12R1/01
Inventor 姚荣清朱英伟高杨赵路曾伟李万钧
Owner YIDU HEC BIOCHEM PHARMA
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