72 results about "Inosine monophosphate" patented technology

Flavivirus, rhabdovirus and paramyxovirus infections may be treated by administering an inhibitor of the enzyme dihydroorotate dehydrogenase such as 6-fluoro-2-(2′-fluoro-1,1′-biphenyl-4-yl)-3-methyl-4-quinolinearcarboxylic acid sodium salt (Brequinar). A synergistic effect can be obtained if an interferon such as interferon α2, interferon α8 or interferon β, or an inhibitor of a second enzyme selected from inosine monophosphate dehydrogenase, guanosine monophosphate synthetase, cytidine triphosphate synthetase and S-adenosylhomocysteine hydrolase, is also administered.

The present invention relates generally to pharmaceutical compositions useful to treat viral diseases. The present invention relates particularly RNA polymerase inhibitors and inosine monophosphate dehydrogenase (IMPDH) inhibitors such as mycophenolate compounds.

The invention relates to a method for measuring the concentration of asymmetric dimethylarginine and a diagnostic reagent kit. The method comprises the following steps of: degrading asymmetric dimethylarginine (ADMA) by adopting dimethylarginine dimethylamino hydrolase to generate dimethylamine and citrulline; reacting the citrulline with adenosine triphosphate (ATP) and aspartate under the action of arginine succinate synthetase to generate adenosine monophosphate (AMP); generating inosine monophosphate (IMP) and ammonia by the AMP under the action of AMP deaminase; generating oxidization nicotinamide adenine dinucleotide (NAD) by the ammonia and deamination NAD under the action of NAD synzyme; and calculating the concentration of the ADMA by detecting the concentration of the NAD. The method and the reagent kit are accurate in results, and high in speed, repeatability and interference resistance, and are convenient to popularize and use.

The invention relates to a seasoning hot pepper for steaming fish and a processing method thereof. The seasoning hot pepper is prepared by the steps: taking the desulfurated pickled capsicum frutescens and the pickled hot green pepper to be matched with other ingredients of ginger, mild water with the temperature of 65 DEG C, citric acid, monosodium glutamate, sodium cyclamate, Acesulfame-K, disodium 5'-Inosine monophosphate, disodium 5'-guanosine monophosphate and ethyl maltol; stirring, standing and sterilizing the substances to obtain the seasoning hot pepper. The seasoning hot pepper has stable quality and low content of sulfur dioxide, better retains the fragility, color and lustre of the hot pepper, as well as the nutrition of the hot pepper, and has longer time and shelf life.

The invention discloses an instant shark skin, which is produced by mixing, boiling and thickening the following raw materials with the following weight percents: 12 to 15 percent of fishy smell removed sharp skin, 3 to 5 percent of compounding material, and 80 to 85 percent of dripping seasoning. The compounding material is prepared by mushroom, black fungus, white fungus, veiled lady and dried scallop with a wet weight proportion of 3:3:6:6:2 after soaked. The dripping seasoning is prepared by the following dripping materials with the following weight percents and water: 10 to 15 percent of abalone juice, 0.3 to 0.5 percent of fresh chicken powder, 0.3 to 0.5 percent of white spirit, 0.1 to 0.3 percent of inosine monophosphate and guanosine monophosphate, 0.05 to 0.1 percent of salt, 0.01 to 0.05 percent of chicken extract, and 2 to 4 percent of starch. The invention has the advantages of convenient use, high nutritional value and good taste. The invention also discloses a preparation method of the instant sharp skin. The method has the advantage of good fishy smell removing effect; in addition, the compounding material and the dripping seasoning for the preparation of the sharp skin taste delicious and are rich in various nutrients.

The invention belongs to the technical field of a biosensor, and discloses an enzyme biosensor for detecting inosine monophosphate(IMP) and a preparation method and an application thereof. The lineardetection range of the enzyme biosensor is 0.313-210 microgram / L, and the detection limit is 0.238 microgram / L. The enzyme biosensor is formed by a reference electrode, a counter electrode and a modified electrode obtained by curing an IMP-sensitive substance recognition film on the surface of a working electrode. The substance recognition film is formed by a composite solution a, a double-enzymecomposite solution b formed by a 5'-nucleotidase solution and an xanthine oxidase solution by the volume ratio 1: 1, and a bovine serum albumin solution by the volume ratio of 1: 1: 1, wherein the composite solution a is composed of a MXene-Ti3C2Tx solution (T is selected from OH, O or F), a chitosan solution, a chloroauric acid solution and a chloroplatinic acid solution by the volume ratio of 12:1.2:1:1. The enzyme biosensor is simple, fast and accurate and high in sensitivity, and can be used for quantitative detection of inosine monophosphate(IMP) in food.

The invention discloses a group of substituted benzoheterocycle amine derivatives and a preparation method and related application thereof as an IMPDH (inosine monophosphate dehydrogenase) inhibitor. The IMPDH inhibitor has good application prospects in virus resistance, immunosuppression, tumor resistance, bacterium resistance, parasite resistance and the like. In the invention, a new-structure IMPDH inhibitor shown by the formula (I) is obtained through the design, synthesis and activity screening study on an active compound targeting IMPDH, and a foundation is laid for the development and application of the compounds as the medicines and medicinal compositions thereof with related effects such as virus resistance, tumor resistance, immunosuppression and the like.

The invention discloses a Raman spectrum-based method for quickly detecting a umami substance inosine monophosphate in fresh fish flesh, relating to the technical field of aquatic product quality and safety detection. The method comprises the steps of 1, measuring a Raman spectrum of a inosine monophosphate standard substance; 2, acquiring a Raman spectrum of a fresh fish flesh sample; 3, measuring inosine monophosphate content by a chemical method; 4, performing Raman spectrum correction; 5, optimizing an inosine monophosphate characteristic peak; 6, calculating the area of the characteristic Raman peak; 7, building a detection model of the inosine monophosphate content. The umami substance inosine monophosphate in the fresh fish flesh is quickly detected by a microscopic confocal Raman spectra technique, and the Raman spectrum-based method for quickly detecting the umami substance inosine monophosphate in the fresh fish flesh which is effective, simple in operation and less in cost and time consumption is found; the detection method is simple, safe and efficient, can meet the industrialization production need and the requirements of consumers on product quality, and satisfies the need of evaluating the flavour and quality of fresh fish flesh products in a practical production and processing process.

Disclosed are phenyl-oxazolyl derivatives having a general formula (I), a preparation method thereof, and an application of the phenyl-oxazolyl derivatives as an inosine monophosphate dehydrogenase (IMPDH) inhibitor.

The invention relates to an adenosine monophosphate (AMP) adenosine deaminase for synthesizing and metabolizing an inosine monophosphate from adenine nucleotide of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application thereof. The AMP adenosine deaminase comprises the proteins shown in SEQ ID No. 1 and SEQ ID No. 2, and the coding gene of the AMP adenosine deaminase corresponds to the nucleotide sequence shown in SEQ ID No. 3 and SEQ ID No.4. According to the invention, the metabolizing way for synthesizing the inosine monophosphate from the adenine nucleotide is researched in detail from the principle, and the cloned DNA containing the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation, and combined transfer; the expression of the gene is biologically synthesized by regulating the inosine monophosphate, the host inosine monophosphate is endowed with high expressivity, an effective way is provided for increasing the yield of the inosine monophosphate, and the invention has a great application prospect.

The present invention relates to a method for preparing a natural kokumi flavor, and more particularly to a method of preparing a natural kokumi flavor using an inosine-5′-monophosphate (IMP) fermented broth or a glutamic acid fermented broth prepared by a two-step fermentation process comprising a first fermentation step for fungal fermentation and a second fermentation step for bacterial fermentation, a natural kokumi flavor prepared by the method, and a food composition comprising the natural kokumi flavor. The natural kokumi flavors prepared according to the method of the present invention are prepared using natural raw materials, and thus are harmless and safe for use in the human body, and may be added to food to produce thick and dense tastes and improve the flavor of the food.

The invention relates to a helicobacter pylori dominant antigen assembly based on CD4+T cell immunity and a preparation method. The dominant antigen assembly comprises the following three components and homologous protein of the three components: inosine monophosphate dehydrogenase, type II citrate synthase and urease B subunit, wherein the amino acid sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The screened dominant antigen assembly based on CD4+T cell immunity has the obvious immune protection effect, has the protection effect superior to that of H.pylori holoprotein antigen, has strong capacity of scavenging helicobacter pylori, and causes extremely slight pathological injuries. The three immunity dominant antigens provided by the invention can induce the body to generate strong immune response reaction aiming at antigens, therefore, through the means of inducing the body to generate the response aiming at the immunoprotecive dominant antigens, or directly immunizing the body by adopting the protective antigens, the effective immune protection effect can be achieved on the helicobacter pylori infection, and the scheme can be used for the further study on the preventive and therapeutic polyvaccines of helicobacter pylori.

The invention discloses a method for increasing adenosine content in a paecilomyces hepiali fermentation mycelium. The method comprises the steps of strain activation, seed liquid preparation, fermentation with a fermentation tank and extraction, and a fermentation medium adopted in the fermentation step comprises 20-25 g / L of soybean cake powder, 10-15 g / L of glucose, 10-15 g / L of sucrose, 0.3-0.5 g / L of potassium dihydrogen phosphate, 0.2-0.4 g / L of MgSO4. 7H2O, 0.25 g / L of anhydrous calcium chloride, 0.4 g / L of soybean oil, 0.02-0.1 g / L of inosine monophosphate (IMP) and the balance of water. The IMP is added into the fermentation medium, so that the adenosine content in the paecilomyces hepiali mycelium powder can be increased, and the yield of the paecilomyces hepiali mycelium powdercan also be increased.

A pharmaceutical composition including an adjuvant effective amount of a protected inosine monophosphate (IMP) compound. The pharmaceutical composition includes the protected IMP compound alone, or in combination with vaccine agents with or without additional adjuvants. The pharmaceutical composition can be utilized as a vaccine composition or can be included with existing vaccine compositions in order to increase a specific T lymphocyte mediated immune response thereto. Various methods relating to the pharmaceutical composition and N the vaccine are described herein. The vaccines can be employed to prevent or treat infections. Additionally, the pharmaceutical compositions not only increase T-cell responses, but also confer, by pretreatment, non-specific protection against a variety of pathogens. This combination of actions is appropriate for enhancing defense against bioterrorism with organisms like smallpox or anthrax.

A method for improving the quality of mutton by using stems and leaves of liquorice comprises the following steps of (1) mixing the following ingredients in percentage by weight: 39%-40% of alfalfa, 19%-21% of withered and yellow liquorice stem and leaf grass meal, 29%-30% of corn, 4.0%-3.9% of cotton seed meal, 3.8%-3.9% of sunflower seed meal, 1.0%-1.1% of salt and 1.0%-1.1% of additives manually or by using a TMR (total mixed ration) machine or preparing the ingredients into total mixed ration particles on the basis of dry matters; and (2) feeding mutton sheep by using the daily ration mixed in the step (1) every morning and every evening at intervals of 10-11 hours. The feeding period is not shorter than 50 days, the content of the dry matters in the liquorice stem and leaf grass meal in the daily ration of the mutton sheep reaches 345-377 grams per day, and the mutton sheep freely eat the ration and freely drink water in the feeding period. By the method, the cholesterol content of the mutton can be reduced obviously, and the content of inosine monophosphate, amino acids and unsaturated fatty acids is increased, so that the quality of the mutton is improved.

The invention relates to a method for determining the activity of 5'-nucleotidase, further to a 5'-nucleotidase detection kit, and belongs to the technical field of medical test determination. According to the method, 5-inosine monophosphate used as a substrate is converted and dehydrogenated by using purine nucleoside phosphorylase and xanthine dehydrogenase as tool enzymes, oxidized coenzyme isreduced to form a reduced coenzyme, and the enzyme reaction rate is calculated by detecting the generation amount of the reduced coenzyme at a wavelength of 340 nm. Compared to the method in the priorart, the method of the present invention has advantages of less reaction steps, less kinds of raw materials, strong alkaline-phosphatase resistance, stable reagent performance, wide linear range, high sensitivity and good accuracy.

The invention relates to an inosine monophosphate (IMP) dehydrogenase from 'Bailing' producing strain Cordyceps sinensis Hirsutella sinensis for participating in anabolism on IMP to obtain a xanthylic acid, a gene for encoding the dehydrogenase and application of the dehydrogenase. The IMP dehydrogenase comprises a protein shown by SEQ ID No.1 and a protein shown by SEQ ID No.2, and the coding gene of the protein shown by SEQ ID No.1 and the coding gene of the protein shown by SEQ ID No.2 respectively correspond to a nucleotide sequence shown by SEQ ID No.3 and a nucleotide sequence shown by SEQ ID No.4. According to the invention, the metabolic pathway of the IMP to synthetize the xanthylic acid is traversed from the principle; the cloning deoxyribonucleic acids (DNA) of the nucleotide sequences provided by the invention can be transferred into engineering bacteria through a transduction method, a transformation method and a conjugal transfer method; and through regulating the expression of the biosynthetic gene of the xanthylic acid, the high expressivity of a host xanthylic acid is endowed, and an effective way for increasing the yield of the xanthylic acid is provided. The IMP dehydrogenase has a great application prospect.

The invention relates to a 5'nucleotidase diagnosis kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining 5'nucleotidase activity concentration, and composition and components of a reagent, and belongs to the technical field of medical test and determination. The kit comprises the following main components: buffer solution, coenzyme, phosphate radical, inosine, inosine monophosphate dehydrogenase, and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the activity concentration of 5'nucleotidase.

A reagent kit for diagnosing 5í»-nucleotidase is composed of buffer liquid, inosine monophosphate, monovalence phosphate, nucleotide phosphatase, xanthine oxidase, peroxidase, stabilizer and reduced chromogen group. A process for measuring the activity of 5í»-nucleotidase includes such steps as proportionally mixing specimen with reagents, enzyme coupling reaction and biochemically analyzing the final resultant to determine the variation in light absorptivity of master wavelength and in turn the activity of 5í»-nucleotidase. Its advantages are high sensitivity and precision, and no pollution.

The invention relates to the technical field of biology, in particular to a PCR (Polymerase Chain Reaction) product qualitative and semiquantitative detecting kit (color PCR kit). The kit comprises a plurality of reagents, such as inosine monophosphate, xanthine oxidase, iodine chloride nitro-tetrazole, hypoxanthineguanine phosphoribesyltransferase, pyrophosphoric acid, and the like. The invention can be used for qualitatively or semiquantitatively detecting a PCR product.

The invention discloses mycophenolic acid derivatives Penicacids A, B, C and their application in preparing immunosuppression medicaments. Penicacids A and B have a specific structure shown in (I), wherein, Penicacids A: R1=CH3, R2=OH; Penicacids B: R1=H, R2=OGlu. And Penicacids C has a specific structure shown in formula (II). Immunosuppressive activity evaluations on Penicacids A, B, and C find that Penicacids A, B and C have an obvious inhibiting effect on inosine monophosphate dehydrogenase (IMPDH), especially the compound Penicacids B which has good inhibitory activity. Therefore, Penicacids A, B, C can be used for preparing immunosuppression medicaments applicable in kidney, liver and other organ transplantations.

Disclosed is a mushroom extract quelite and its preparing process, which comprises 18.45% of mushroom extract, 33.21% of table salt, 23.99% of malt extract, 17.53% of frosted sugar, 2.21% of yeast extract, 0.37% of white pepper powder, 3.69% of sodiam inosine monophosphate 0.55% of sodium succinate. And the making process comprises the steps of lixiviating mushroom stems with water, vacuum concentrating, spray drying centrifugally, charging findings, mixing, granulating, drying and screening.

The invention relates to an adenosine deaminase diagnosis kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining adenosine deaminase activity concentration, and composition and components of a reagent, and belongs to the technical field of medical test and determination. The kit comprises the following main components: buffer solution, coenzyme, adenosine, adenosine triphosphate, inosine kinase, inosine monophosphate dehydrogenase and a stabilizer. The method for determining adenosine deaminase activity concentration comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the activity concentration of adenosine deaminase.

The invention relates to a method for determining the activity of adenosine deaminase, and also the reagent kit for adenosine deaminase diagnosis. The reagent kit comprises cushioning solution, adenosine, inosine monophosphate, oxidized type coenzyme, guanosine monophosphate reductase, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the activity of the adenosine deaminase can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

The invention discloses a high-accuracy 5'-nucleotidase assay kit and relates to the technical field of bioassay. The kit comprises a reagent R1 and a reagent R2 which are independent from each other,wherein the reagent R1 is N-acetylpiperazine-N-2-ethanesulfonic acid, 4-aminoantipyrine, purine nucleoside phosphorylase, xanthine oxidase, peroxidase and sodium azide; and the reagent R2 is acetylpiperazine-N-2-ethanesulfonic acid, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, 5'-inosine monophosphate disodium salt and sodium azide. In the application process of the kit, by controlling the mass ratio of the components, the stability of the kit can be improved and assay accuracy and precision can be also improved, and in addition, the kit is applicable to fully automatic biochemical analyzers, is simple to operate, high in automation degree and applicable to wide clinical application.

The invention relates to a modified IMPDH polypeptide wherein the IMPDH polypeptide has a histidine tag and where the subdomain of the IMPDH polypeptide is modified so that the rate stability of the histidine-tagged, modified IMPDH polypeptide is maintained relative to the wild-type IMPDH polypeptide. The invention also relates to a method, nucleic acid molecules, vectors, and host cells for producing such a histidine-tagged, modified IMPDH polypeptide, and to kits comprising the histidine-tagged, modified IMPDH polypeptide.

The invention relates to a method for detecting the freshness of royal jelly. The specific determination method is: Quantitative determination of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosinic acid (IMP), secondary Xanthine nucleoside (HxR), hypoxanthine (Hx), adenine (adenine) and adenosine (adenosine); calculate the cumulative amount of adenine, adenosine, HxR and Hx and the above 8 substances (ATP and nucleic acid The ratio (F value) of the quality sum of degradation products); Utilize the F value size of royal jelly to detect the freshness of royal jelly, judge the quality of royal jelly. Compared with the existing method for judging the quality of royal jelly, the method can judge the quality and freshness of royal jelly more accurately and sensitively, can improve the current quality standard of royal jelly, and effectively monitor the quality of royal jelly.

The invention relates to a helicobacter pylori dominant antigen assembly based on CD4+T cell immunity and a preparation method. The dominant antigen assembly comprises the following three components and homologous protein of the three components: inosine monophosphate dehydrogenase, type II citrate synthase and urease B subunit, wherein the amino acid sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The screened dominant antigen assembly based on CD4+T cell immunity has the obvious immune protection effect, has the protection effect superior to that of H.pylori holoprotein antigen, has strong capacity of scavenging helicobacter pylori, and causes extremely slight pathological injuries. The three immunity dominant antigens provided by the invention can induce the body to generate strong immune response reaction aiming at antigens, therefore, through the means of inducing the body to generate the response aiming at the immunoprotecive dominant antigens, or directly immunizing the body by adopting the protective antigens, the effective immune protection effect can be achieved on the helicobacter pylori infection, and the scheme can be used for the further study on the preventive and therapeutic polyvaccines of helicobacter pylori.

The present disclosure relates to a novel protein variant having an activity of exporting 5′-inosine monophosphate, a microorganism comprising the protein variant, and a method for preparing 5′-inosine monophosphate using the microorganism.