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High-accuracy 5'-nucleotidase assay kit

A nucleotidase and high-accuracy technology, which is applied in the field of high-accuracy 5'-nucleotidase assay kits, can solve the problems of unstable reagents and low assay accuracy, and achieve improved sensitivity and automation High, the effect of improving accuracy

Active Publication Date: 2020-05-12
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a 5'-nucleotidase assay kit in order to overcome the defects of unstable reagents and low assay accuracy in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 A kind of high accuracy 5'-nucleotidase assay kit

[0054] Including reagent R1 and reagent R2 which are independent of each other, and its composition and corresponding content are:

[0055] Reagent R1:

[0056]

[0057] Reagent R2:

[0058]

Embodiment 2

[0059] Embodiment 2 A kind of high accuracy 5'-nucleotidase assay kit

[0060] Including reagent R1 and reagent R2 which are independent of each other, and its composition and corresponding content are:

[0061] Reagent R1:

[0062]

[0063]

[0064] Reagent R2:

[0065]

Embodiment 3

[0066] Embodiment 3 A kind of high accuracy 5'-nucleotidase assay kit

[0067] Including reagent R1 and reagent R2 which are independent of each other, and its composition and corresponding content are:

[0068] Reagent R1:

[0069]

[0070] Reagent R2:

[0071]

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Abstract

The invention discloses a high-accuracy 5'-nucleotidase assay kit and relates to the technical field of bioassay. The kit comprises a reagent R1 and a reagent R2 which are independent from each other,wherein the reagent R1 is N-acetylpiperazine-N-2-ethanesulfonic acid, 4-aminoantipyrine, purine nucleoside phosphorylase, xanthine oxidase, peroxidase and sodium azide; and the reagent R2 is acetylpiperazine-N-2-ethanesulfonic acid, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, 5'-inosine monophosphate disodium salt and sodium azide. In the application process of the kit, by controlling the mass ratio of the components, the stability of the kit can be improved and assay accuracy and precision can be also improved, and in addition, the kit is applicable to fully automatic biochemical analyzers, is simple to operate, high in automation degree and applicable to wide clinical application.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a highly accurate 5'-nucleotidase assay kit. Background technique [0002] 5'-nucleotidase (5'-NT), the full name is 5'-ribonucleotide phosphohydrolase. 5'-Nucleotidase is a hydrolase with low substrate specificity and can act on a variety of nucleotides. Mainly involved in the catabolism of nucleic acids, can specifically catalyze nucleoside 5'-nucleotidase and inosine nucleotides, 5'-nucleotidase in the movement of the human body, cell growth and development, fibrin synthesis, improve Epidermal or endothelial shielding function, recirculation and adhesion of neurotransmitting lymphocytes, and immune response all play important roles. [0003] 5'-nucleotidases are widely present in the liver and various tissues, and elevated serum 5'-nucleotidases are mainly seen in liver-pancreatic system diseases and some malignant tumors, so they have relatively specific diagno...

Claims

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Application Information

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IPC IPC(8): C12Q1/48C12Q1/44C12Q1/28C12Q1/26
CPCC12Q1/48C12Q1/44C12Q1/28C12Q1/26G01N2333/9029G01N2333/91142C12Q2326/96Y02A50/30
Inventor 林耀文陈青松
Owner 浙江夸克生物科技有限公司
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