Enzyme biosensor for detecting inosine monophosphate(IMP) and preparation method and application thereof

A biosensor and inosinic acid technology, applied in the field of biosensors, can solve the problems of long time-consuming, complicated operation, high detection cost, etc., and achieve the effects of improved reaction speed, simple preparation process, and strong anti-interference ability

Active Publication Date: 2019-11-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems of high detection cost, long time-consuming and complicated operation of the existing methods for detecting inosinic acid, the primary purpose of the present invention is to provide an enzyme biosensor for detecting inosinic acid with good selectivity, sensitivity and stability

Method used

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  • Enzyme biosensor for detecting inosine monophosphate(IMP) and preparation method and application thereof
  • Enzyme biosensor for detecting inosine monophosphate(IMP) and preparation method and application thereof
  • Enzyme biosensor for detecting inosine monophosphate(IMP) and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Enzyme biosensor for detecting inosinic acid, its overall structure is as follows figure 1 As shown, it is composed of a reference electrode 2, a counter electrode 3 and a modified electrode 1. The modified electrode 1 is composed of a working electrode 1-1 and a substance recognition film 1-2 solidified on the surface of the working electrode. Among them, the inosinic acid-sensitive Substance recognition membrane 1-2 made of MXene-Ti 3 C 2 Prepared from Tx solution, chitosan solution, chloroauric acid solution, chloroplatinic acid solution, 5'-nucleotidase solution, xanthine oxidase solution and bovine serum albumin solution, put the above enzyme biosensor into the In the test solution 4, the content of inosinic acid in the test solution 4 can be detected.

[0043] The preparation process of the modified electrode 1 is as follows: figure 2 Shown, concrete preparation method step is:

[0044] (1) Working electrode pretreatment. The working electrode was polished t...

Embodiment 2

[0050] The enzyme biosensor for detecting inosinic acid, its preparation steps are as follows:

[0051] (1) A glassy carbon electrode with a diameter of 3 mm was polished to a mirror surface on a polishing cloth with alumina powders with a diameter of 0.3 μm and 0.05 μm in sequence, then rinsed with ultrapure water, and then ultrasonically treated in ultrapure water for 1 min, and left to dry After drying, the glassy carbon electrode was placed in potassium ferricyanide solution (made by K 3 [Fe(CN) 6 ], K 4 [Fe(CN) 6 ] and KCl in a molar ratio of 1:1:100), at -0.2 to 0.6V, use cyclic voltammetry to scan 4 circles to activate the electrode, take it out and rinse it with ultrapure water, and dry it with nitrogen to get Pretreated glassy carbon electrodes.

[0052] (2) 125mgMXene-Ti 3 C 2 The Tx material was dispersed in 100mL ultrapure water to obtain MXene-Ti with a concentration of 1.25mg / mL 3 C 2 Tx solution; Adopt 1wt% acetic acid solution preparation concentration ...

Embodiment 3

[0060] The enzyme biosensor for detecting inosinic acid, its preparation steps are as follows:

[0061] (1) A glassy carbon electrode with a diameter of 3 mm was polished to a mirror surface on a polishing cloth with alumina powders with a diameter of 0.3 μm and 0.05 μm in sequence, then rinsed with ultrapure water, and then ultrasonically treated in ultrapure water for 1 min, and left to dry After drying, the glassy carbon electrode was placed in potassium ferricyanide solution (made by K 3 [Fe(CN) 6 ], K 4 [Fe(CN) 6 ] and KCl in a molar ratio of 1:1:100), at -0.2 to 0.6V, use cyclic voltammetry to scan 4 circles to activate the electrode, take it out and rinse it with ultrapure water, and dry it with nitrogen to get Pretreated glassy carbon electrodes.

[0062] (2) 125mgMXene-Ti 3 C 2 The Tx material was dispersed in 100mL ultrapure water to obtain MXene-Ti with a concentration of 1.25mg / mL 3 C 2 Tx solution; Adopt 1wt% acetic acid solution preparation concentration ...

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Abstract

The invention belongs to the technical field of a biosensor, and discloses an enzyme biosensor for detecting inosine monophosphate(IMP) and a preparation method and an application thereof. The lineardetection range of the enzyme biosensor is 0.313-210 microgram/L, and the detection limit is 0.238 microgram/L. The enzyme biosensor is formed by a reference electrode, a counter electrode and a modified electrode obtained by curing an IMP-sensitive substance recognition film on the surface of a working electrode. The substance recognition film is formed by a composite solution a, a double-enzymecomposite solution b formed by a 5'-nucleotidase solution and an xanthine oxidase solution by the volume ratio 1: 1, and a bovine serum albumin solution by the volume ratio of 1: 1: 1, wherein the composite solution a is composed of a MXene-Ti3C2Tx solution (T is selected from OH, O or F), a chitosan solution, a chloroauric acid solution and a chloroplatinic acid solution by the volume ratio of 12:1.2:1:1. The enzyme biosensor is simple, fast and accurate and high in sensitivity, and can be used for quantitative detection of inosine monophosphate(IMP) in food.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to an enzyme biosensor for detecting inosinic acid, its preparation method and application, which are used for the detection of inosinic acid (IMP) in food. Background technique [0002] Inosinic acid (IMP) is a single nucleotide and is a metabolic intermediate of ATP in organisms. Inosinic acid and its salts can produce umami, which is one of the important flavor substances in meat products. It can also act synergistically with sodium glutamate to double the umami, and is a commonly used flavoring agent. In addition, inosinic acid is also an important indicator internationally recognized to measure the freshness of meat. At present, methods for quantitative detection of inosinic acid include spectrophotometry, high performance liquid chromatography, etc., but these methods have problems such as high detection cost, long time consumption, and complicated operation. In comparison...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3277G01N27/3278G01N27/48
Inventor 刘源刘静思王广现姜水
Owner SHANGHAI JIAO TONG UNIV
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