Non-enzyme sensor based on nitrogen-doped graphene and preparation method and application thereof

A nitrogen-doped graphene and enzyme sensor technology is applied in the field of biosensors to achieve the effects of improving reaction speed, stable performance and good selectivity

Inactive Publication Date: 2017-10-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the application of nitrogen-doped graphene-based enzyme-free sensors for hydrogen peroxide detection.

Method used

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  • Non-enzyme sensor based on nitrogen-doped graphene and preparation method and application thereof
  • Non-enzyme sensor based on nitrogen-doped graphene and preparation method and application thereof
  • Non-enzyme sensor based on nitrogen-doped graphene and preparation method and application thereof

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Embodiment 1

[0035] A method for preparing an enzyme-free biosensor based on nitrogen-doped graphene in this embodiment, the specific preparation steps are as follows:

[0036] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished into a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the treated glassy carbon electrode was placed in 0.5mol / L H 2 SO 4 In the solution, the electrochemical redox is carried out at the potential window of -0.3-1.5V, the glassy carbon electrode is activated and the residue on the electrode surface is removed.

[0037] (2) Take 100mg of graphite oxide in a beaker, add 50mL of deionized water and ultrasonically strip it for 4h, then add 6.25mL of FeCl3 ·6H 2 O solution and 235 μL of pyrrole monomer were placed in the reactor at 40°C for 1 hour and stirred at a constant temp...

Embodiment 2

[0044] A method for preparing an enzyme-free biosensor based on nitrogen-doped graphene in this embodiment, the specific preparation steps are as follows:

[0045] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished into a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the treated glassy carbon electrode was placed in 0.5mol / L H 2 SO 4 In the solution, the electrochemical redox is carried out at the potential window of -0.3-1.5V, the glassy carbon electrode is activated and the residue on the electrode surface is removed.

[0046] (2) Take 100mg of graphite oxide in a beaker, add 50mL of deionized water and ultrasonically strip it for 4h, then add 6.25mL of FeCl 3 ·6H 2 O solution and 235 μL of pyrrole monomer were placed in the reactor at 40°C for 1 hour and stirred at a constant te...

Embodiment 3

[0053] A method for preparing an enzyme-free biosensor based on nitrogen-doped graphene in this embodiment, the specific preparation steps are as follows:

[0054] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished into a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the treated glassy carbon electrode was placed in 0.5mol / L H 2 SO 4 In the solution, the electrochemical redox is carried out at the potential window of -0.3-1.5V, the glassy carbon electrode is activated and the residue on the electrode surface is removed.

[0055] (2) Take 100mg of graphite oxide in a beaker, add 50mL of deionized water and ultrasonically strip it for 4h, then add 6.25mL of FeCl 3 ·6H 2 O solution and 235 μL of pyrrole monomer were placed in the reactor at 40°C for 1 hour and stirred at a constant te...

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Abstract

The invention belongs to the technical field of biological sensors, and discloses a non-enzyme sensor based on nitrogen-doped graphene and a preparation method and application thereof. The sensor consists of a reference electrode, a counter electrode and a modified working electrode, wherein the modified working electrode consists of a working electrode and a material recognizing membrane cured on the surface of the working electrode, and the material recognizing membrane consists of a nitrogen-doped graphene composite material and perfluorinated sulfonic acid resin. The non-enzyme sensor provided by the invention is applied to the detection of hydrogen peroxide, is wide in detection range, is low in detection limit, and has stable performance and a good application prospect; a reaction is implemented in a neutral environment at the room temperature.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to an enzyme-free sensor based on nitrogen-doped graphene and its preparation method and application. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) are ubiquitous in nature and have important applications in chemical, textile, environmental protection, medical and food production. In addition, it is also a by-product of many oxidases participating in the reaction in the organism, and it often exists in the form of a reaction intermediate, so that it can control the metabolism in the organism and regulate cell apoptosis in the organism. The occurrence of various diseases is related to the excessive hydrogen peroxide content in the body. closely related. Therefore, the analysis and determination of hydrogen peroxide is of great significance in biological, medical and clinical diagnostic research. The current research methods for hydrogen peroxide detect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/333G01N27/327
CPCG01N27/48G01N27/327G01N27/333
Inventor 朴金花陈东霞张力姜建国
Owner SOUTH CHINA UNIV OF TECH
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