Preparation method and application of electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres

An immunosensor, molybdenum disulfide technology, applied in the field of nanomaterials and electrochemical analysis, can solve the problems of weak dispersion and conductivity, achieve short response time, good electron transfer ability and catalytic performance, and improve sensitivity

Inactive Publication Date: 2018-09-14
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the catalytic activity of molybdenum disulfide is much higher than that of redox graphene, its dispersion and conductivity are weaker than those of redox graphene.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 A preparation method of an electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres, the steps are as follows:

[0034] (1) Preparation of palladium-doped molybdenum disulfide nanospheres

[0035] 1) Add 0.5mmol of ammonium molybdate tetrahydrate, 10mmol of thiourea and 0.005mmol of polyvinylpyrrolidone to 35mL of ultrapure water in turn, and magnetically stir to form a homogeneous solution A;

[0036] 2) Transfer solution A to a high-temperature reactor, react at 220ºC for 18 hours, centrifuge, wash with ultrapure water and absolute ethanol to remove impurities, and freeze-dry the product in a freeze dryer to obtain Molybdenum disulfide nanospheres;

[0037]3) Dissolve 30 mmol of hexadecyltrimethylammonium bromide in 10 mL of ultrapure water, and sonicate for 10 min to obtain a uniform solution B; then add 1 mg of molybdenum disulfide nanospheres to solution B, and the mixed solution becomes Turn into gray black, continue ul...

Embodiment 2

[0045] Embodiment 2 A preparation method of an electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres, the steps are as follows:

[0046] (1) Preparation of palladium-doped molybdenum disulfide nanospheres

[0047] 1) Add 1.0 mmol of ammonium molybdate tetrahydrate, 15 mmol of thiourea and 0.010 mmol of polyvinylpyrrolidone to 35 mL of ultrapure water in sequence, and magnetically stir to form a homogeneous solution A;

[0048] 2) Transfer solution A to a high-temperature reactor, react at 220ºC for 18 hours, centrifuge, wash with ultrapure water and absolute ethanol to remove impurities, and freeze-dry the product in a freeze dryer to obtain Molybdenum disulfide nanospheres;

[0049] 3) Dissolve 50 mmol of cetyltrimethylammonium bromide in 10 mL of ultrapure water and sonicate for 15 min to obtain a uniform solution B; then add 3 mg of molybdenum disulfide nanospheres to solution B, and mix the solution Turn into gray-black, continue ultrason...

Embodiment 3

[0057] Embodiment 3 A preparation method of an electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres, the steps are as follows:

[0058] (1) Preparation of palladium-doped molybdenum disulfide nanospheres

[0059] 1) Add 1.5 mmol of ammonium molybdate tetrahydrate, 20 mmol of thiourea and 0.015 mmol of polyvinylpyrrolidone to 35 mL of ultrapure water in sequence, and stir magnetically to form a homogeneous solution A;

[0060] 2) Transfer solution A to a high-temperature reactor, react at 220ºC for 18 hours, centrifuge, wash with ultrapure water and absolute ethanol to remove impurities, and freeze-dry the product in a freeze dryer to obtain Molybdenum disulfide nanospheres;

[0061] 3) Dissolve 70 mmol of cetyltrimethylammonium bromide in 10 mL of ultrapure water and sonicate for 20 min to obtain a uniform solution B; then add 5 mg of molybdenum disulfide nanospheres to solution B, and mix the solution Turn into gray-black, continue ultrason...

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PUM

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Abstract

The invention relates to a preparation method and application of an electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres, which belongs to the technical field of nanomaterials and electrochemical analysis. By adopting the electrochemical immunosensor prepared by molybdenum disulfide nanospheres modified by palladium nano particles doped cetyltrimethylammonium bromide, the high-sensitivity detection of N-terminal B-type N-terminal pro-brain natriuretic peptide is realized.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres, and belongs to the field of nanometer materials and electrochemical analysis. Background technique [0002] N-terminal pro-B-type natriuretic peptide plays a very important role in the screening, efficacy evaluation and prognosis judgment of heart failure. As the most sensitive and specific indicator of cardiac dysfunction, it can be used as a new indicator for early diagnosis of diastolic heart failure, and has important clinical significance. How to efficiently and accurately realize the sensitive detection of N-terminal pro-B-type natriuretic peptide is an urgent problem to be solved. [0003] Electrochemical immunoassay immunosensing analysis is a kind of analytical method for the specific combination of antigen and antibody. It has the advantages of high analytical sensitivity, strong specific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/308G01N27/3278
Inventor 李月云高增强张春燕张栓王平刘青董云会王粤博
Owner SHANDONG UNIV OF TECH
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