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Adenosine deaminase diagnosis kit and method for determining adenosine deaminase activity concentration

A technology of adenosine deaminase and inosine monophosphate dehydrogenase, which is applied in the field of medical inspection and measurement, and can solve problems such as difficult promotion, application and measurement

Inactive Publication Date: 2010-01-06
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot be determined by the visible light analyzer in general hospitals, but requires the use of a special ultraviolet light analyzer, so it is difficult to practically popularize and apply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The adenosine deaminase diagnostic reagent of this embodiment is a single reagent, comprising:

[0040] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0041] Stabilizer 500mmol / L

[0042] Coenzyme 3mmol / L

[0043] Inosine kinase 6000U / L

[0044] Inosine monophosphate dehydrogenase 8000U / L

[0045] Adenosine 6mmol / L

[0046] Adenosine triphosphate 9mmol / L

[0047] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0048] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, the volume ratio of the tested adenosine deaminase sample to the reagent is 1 / 25, the reaction direction is positive reaction (rising reaction), the delay time is about 1 minute, the detection time is about 2 minutes, and the the...

Embodiment 2

[0051] The adenosine deaminase diagnostic reagent of this embodiment is a double reagent, comprising:

[0052] Reagent 1

[0053] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0054] Stabilizer 50mmol / L

[0055] Coenzyme 3mmol / L

[0056] Adenosine 6mmol / L

[0057] Adenosine triphosphate 9mmol / L

[0058] Reagent 2

[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0060] Stabilizer 500mmol / L

[0061] Inosine kinase 6000U / L

[0062] Inosine monophosphate dehydrogenase 8000U / L

[0063] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0064]Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, test adenosine deaminase sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the reaction direction is positive reactio...

Embodiment 3

[0067] The adenosine deaminase diagnostic reagent of this embodiment is three reagents, comprising:

[0068] Reagent 1

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 50mmol / L

[0071] Coenzyme 3mmol / L

[0072] Adenosine 6mmol / L

[0073] Adenosine triphosphate 9mmol / L

[0074] Reagent 2

[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0076] Stabilizer 500mmol / L

[0077] Inosine monophosphate dehydrogenase 8000U / L

[0078] Reagent 3

[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0080] Stabilizer 500mmol / L

[0081] Inosine kinase 6000U / L

[0082] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0083] When measuring the activity concentration of adenosine deaminase, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorban...

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Abstract

The invention relates to an adenosine deaminase diagnosis kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining adenosine deaminase activity concentration, and composition and components of a reagent, and belongs to the technical field of medical test and determination. The kit comprises the following main components: buffer solution, coenzyme, adenosine, adenosine triphosphate, inosine kinase, inosine monophosphate dehydrogenase and a stabilizer. The method for determining adenosine deaminase activity concentration comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the activity concentration of adenosine deaminase.

Description

technical field [0001] The invention relates to a diagnostic kit for adenosine deaminase, and at the same time, the invention also relates to a method for measuring the activity concentration of adenosine deaminase, which belongs to the technical field of medical examination and measurement. Background technique [0002] Medical research shows that adenosine deaminase is a nucleic acid metabolizing enzyme that has an important relationship with the body's cellular immune activity. Therefore, the determination of adenosine deaminase activity is used as an important diagnostic marker for the differential diagnosis of benign and malignant hydrothorax, cerebrospinal fluid, severe combined immunodeficiency disease (SCID), acute and chronic hepatitis, liver cirrhosis, liver cancer and other diseases. [0003] The methods for determining the activity of adenosine deaminase mainly include radionuclide method, physical method and biochemical method. As far as the applicant knows, th...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/25C12Q1/00C12Q1/68
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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