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Method for producing epsilon-poly-L-lysine

A production method and technology for lysine, applied in the field of fermentation engineering, can solve the problems of not studying the influence of glycine fermentation performance, etc., and achieve the effects of increasing the metabolic activity of the production strain, improving the yield, and increasing the accumulation amount.

Active Publication Date: 2012-02-29
ZHEJIANG SILVER ELEPHANT BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned patent documents mainly focus on the production strain and fermentation process of ε-poly-L-lysine, but none of them studies the influence of glycine on its fermentation performance, which is essentially different from this patent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A production method of ε-poly-L-lysine, the steps are as follows:

[0022] Insert the spores of 1-2 ring amylase chromogenic Streptomyces or albicans into 100mL culture medium, shake culture at 30°C for 30h; then insert 100mL fermentation medium (initial pH 6.8), cultured at 30°C for 72h. During the fermentation process, the maximum yield of ε-poly-L-lysine was 0.4g / L.

Embodiment 2

[0024] A production method of ε-poly-L-lysine, the steps are as follows:

[0025] Insert the spores of Streptomyces chromogenes or Streptomyces albicans with 1-2 rings of amylase in 100mL culture medium, the medium contains glycine 3g / L, shake culture at 30°C for 30h; then inoculate with 6% (V / V) The amount was added to 100mL fermentation medium (initial pH 6.8), and cultured at 30°C for 72h. During the fermentation process, the maximum yield of ε-poly-L-lysine was 0.5g / L.

Embodiment 3

[0027] A production method of ε-poly-L-lysine, the steps are as follows:

[0028] Insert the spores of Streptomyces chromogenes or Streptomyces albicans with 1-2 rings of amylase in 100mL culture medium, shake and culture at 30°C for 48h; In 100mL of fermentation medium, the initial pH was 4.5, and cultured at 30°C for 72h. During the fermentation process, the maximum yield of ε-poly-L-lysine was 0.7g / L.

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Abstract

The invention relates to a method for producing epsilon-poly-L-lysine. The method is characterized in that a medium containing 1-10g / L of glycine is added at an early or middle stage of culture. By adding glycine in a fermentation process in the invention, glycine which enters a folic acid metabolism approach allows sufficient one-carbon groups to be supplied to a biosynthesis process, the anabolism activity of bacterial strains to be improved, the synthesis of a precursor L-lysine and epsilon-poly-L-lysine to be increased, and the accumulation amount of epsilon-poly-L-lysine to be 20-50% higher than the accumulation amount obtained through original technologies.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, in particular to a production method of ε-poly-L-lysine. Background technique [0002] ε-poly-L-lysine (ε-PL) is a microbial extracellular linear polymer, which consists of only 25-35 L-lysine residues linked by α-carboxyl and ε-amino groups. In 1977, Shima and Sakai first discovered this amino acid polymer in the fermentation serum of Streptomyces albulus NBRC 14147 strain. ε-poly-L-lysine is a polycationic polypeptide that is safe and non-toxic to the human body. It can inhibit Gram-negative bacteria, Gram-positive bacteria, fungi and even some viruses, and has a wide antibacterial spectrum. Since the safety of ε-PL was proved by mouse experiments, it has been widely used as a natural food preservative in Japan, South Korea, the United States and other countries. ε-PL and its derivatives have a wide range of applications. In addition to being used for food preservation, they can also b...

Claims

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Application Information

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IPC IPC(8): C12P13/02C12R1/47C12R1/525
Inventor 贾士儒王甜谭之磊韩培培戴玉杰钟成宋帅张雪
Owner ZHEJIANG SILVER ELEPHANT BIO ENG
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