Process for abstracting mycelium polysaccharide by cordyceps fermentation

A technology of Cordyceps sinensis mycelium and mycelium polysaccharides, which is applied in the field of extraction of Cordyceps sinensis mycelium polysaccharides, can solve the problems of integration, unfavorable fierce competition, unfavorable international market, etc., and achieve the effect of simple steps and low cost

Inactive Publication Date: 2008-11-19
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, most of the relevant Cordyceps sinensis products on the market are crude mycelium products that can be directly used at one time, which

Method used

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  • Process for abstracting mycelium polysaccharide by cordyceps fermentation
  • Process for abstracting mycelium polysaccharide by cordyceps fermentation
  • Process for abstracting mycelium polysaccharide by cordyceps fermentation

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Embodiment 1

[0034] Example 1, Extraction and Purification of Cordyceps Sinensis Fermented Mycelium Polysaccharide

[0035] 1) Preparation of Cordyceps sinensis fermentation mycelium

[0036] The strains isolated and purified from natural Cordyceps sinensis and identified by molecular biology as true species of Cordyceps sinensis are inserted into the semi-synthetic medium of Cordyceps sinensis, fermented according to conventional methods, and mycelium is collected. The Cordyceps sinensis semi-synthetic medium was prepared as follows: take 50.0 grams of sucrose, 10.0 grams of peptone, 3.0 grams of yeast extract, 1.0 grams of potassium dihydrogen phosphate, and 0.5 grams of magnesium sulfate, and dilute to 1000 milliliters with distilled water.

[0037] 2) Extraction of crude polysaccharides from Cordyceps sinensis mycelium

[0038] Flowchart of the extraction and purification of polysaccharides from Cordyceps sinensis mycelium, such as figure 1 shown.

[0039] With the Cordyceps sinensi...

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Abstract

The invention discloses a method for extracting and purifying polysaccharide from a fermented mycelium of Cordyceps sinensis. The method comprises the following steps of: using the fermented mycelium of Cordyceps sinensis as a raw material to produce crude polysaccharide of the Cordyceps sinensis via degreasing, aqueous extraction, concentration and alcohol precipitation; then, redissolving the crude polysaccharide into distilled water, deproteinizing the crude polysaccharide by Sevag, decoloring the deproteinized crude polysaccharide by H2O2, dialyzing the decolored crude polysaccharide, and precipitating the dialyzed crude polysaccharide by alcohol to produce primarily purified polysaccharide; finally, purifying the primarily purified polysaccharide by ion-exchange chromatography, collecting different produced compositions stepwisely, dialyzing the compositions, precipitating the dialyzed compositions by alcohol, drying the precipitated compositions to produce the polysaccharide of the Cordyceps sinensis after the precipitated compositions are orderly washed by absolute ethyl alcohol, acetone, aether and so on. In the produced polysaccharide of the Cordyceps sinensis, MPS2 is identified to be homogeneous polysaccharide by ultraviolet absorption spectrum and gel chromatography, with high content of total sugar. The method has the advantages of simple and convenient extraction steps and low cost.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides from fermented mycelium of Cordyceps sinensis. Background technique [0002] Polysaccharide is a macromolecular active compound formed by the polymerization of multiple monosaccharides or their derivatives. It has been confirmed by pharmacological research and clinical application to have anti-tumor, anti-inflammatory, anti-coagulation, anti-virus, anti-radiation, hypoglycemic, hypolipidemic, etc. biological activity. Polysaccharides are gradually becoming one of the important directions in the development of new drugs. At present, lentinan, polyporus polysaccharide, and wolfberry polysaccharide have been used in clinical practice at home and abroad. [0003] Cordyceps sinensis [Cordyceps sinensis (Berk.) Sacc.] is a complex of insects and fungi formed by Cordyceps sinensis parasitizing on the larvae of the Lepidoptera (Lepidoptera) bat moth family (Hepialidae), especially the Cordyceps ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 姚一建董彩虹
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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